Intercropping Alters the Soil Microbial Diversity and Community to Facilitate Nitrogen Assimilation: A Potential Mechanism for Increasing Proso Millet Grain Yield
Intercropping of cereals and legumes has been used in modern agricultural systems, and the soil microorganisms associated with legumes play a vital role in organic matter decomposition and nitrogen (N) fixation. This study investigated the effect of intercropping on the rhizosphere soil microbial co...
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doaj-4e99dd8a64734707b31cd59f1bf9f5172020-11-25T04:12:03ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-11-011110.3389/fmicb.2020.601054601054Intercropping Alters the Soil Microbial Diversity and Community to Facilitate Nitrogen Assimilation: A Potential Mechanism for Increasing Proso Millet Grain YieldKe Dang0Xiangwei Gong1Guan Zhao2Honglu Wang3Aliaksandr Ivanistau4Baili Feng5College of Agronomy, State Key Laboratory of Crop Stress Biology in Arid Areas/Northwest A & F University, Yangling, ChinaCollege of Agronomy, State Key Laboratory of Crop Stress Biology in Arid Areas/Northwest A & F University, Yangling, ChinaCollege of Agronomy, State Key Laboratory of Crop Stress Biology in Arid Areas/Northwest A & F University, Yangling, ChinaCollege of Agronomy, State Key Laboratory of Crop Stress Biology in Arid Areas/Northwest A & F University, Yangling, ChinaBelarusian State Agricultural Academy, Gorki, BelarusCollege of Agronomy, State Key Laboratory of Crop Stress Biology in Arid Areas/Northwest A & F University, Yangling, ChinaIntercropping of cereals and legumes has been used in modern agricultural systems, and the soil microorganisms associated with legumes play a vital role in organic matter decomposition and nitrogen (N) fixation. This study investigated the effect of intercropping on the rhizosphere soil microbial composition and structure and how this interaction affects N absorption and utilization by plants to improve crop productivity. Experiments were conducted to analyze the rhizosphere soil microbial diversity and the relationship between microbial composition and N assimilation by proso millet (Panicum miliaceum L.) and mung bean (Vigna radiata L.) from 2017 to 2019. Four different intercropping row arrangements were evaluated, and individual plantings of proso millet and mung bean were used as controls. Microbial diversity and community composition were determined through Illumina sequencing of 16S rRNA and internal transcribed spacer (ITS) genes. The results indicated that intercropping increased N levels in the soil–plant system and this alteration was strongly dependent on changes in the microbial (bacterial and fungal) diversities and communities. The increase in bacterial alpha diversity and changes in unique operational taxonomic unit (OTU) numbers increased the soil N availability and plant N accumulation. Certain bacterial taxa (such as Proteobacteria) and fungal taxa (such as Ascomycota) were significantly altered under intercropping and showed positive responses to increased N assimilation. The average grain yield of intercropped proso millet increased by 13.9–50.1% compared to that of monoculture proso millet. Our data clearly showed that intercropping proso millet with mung bean altered the rhizosphere soil microbial diversity and community composition; thus, this intercropping system represents a potential mechanism for promoting N assimilation and increasing grain yield.https://www.frontiersin.org/articles/10.3389/fmicb.2020.601054/fullcereal–legume intercroppingsoil microbial communitysoil–plant systemN availabilitygrain yield |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ke Dang Xiangwei Gong Guan Zhao Honglu Wang Aliaksandr Ivanistau Baili Feng |
spellingShingle |
Ke Dang Xiangwei Gong Guan Zhao Honglu Wang Aliaksandr Ivanistau Baili Feng Intercropping Alters the Soil Microbial Diversity and Community to Facilitate Nitrogen Assimilation: A Potential Mechanism for Increasing Proso Millet Grain Yield Frontiers in Microbiology cereal–legume intercropping soil microbial community soil–plant system N availability grain yield |
author_facet |
Ke Dang Xiangwei Gong Guan Zhao Honglu Wang Aliaksandr Ivanistau Baili Feng |
author_sort |
Ke Dang |
title |
Intercropping Alters the Soil Microbial Diversity and Community to Facilitate Nitrogen Assimilation: A Potential Mechanism for Increasing Proso Millet Grain Yield |
title_short |
Intercropping Alters the Soil Microbial Diversity and Community to Facilitate Nitrogen Assimilation: A Potential Mechanism for Increasing Proso Millet Grain Yield |
title_full |
Intercropping Alters the Soil Microbial Diversity and Community to Facilitate Nitrogen Assimilation: A Potential Mechanism for Increasing Proso Millet Grain Yield |
title_fullStr |
Intercropping Alters the Soil Microbial Diversity and Community to Facilitate Nitrogen Assimilation: A Potential Mechanism for Increasing Proso Millet Grain Yield |
title_full_unstemmed |
Intercropping Alters the Soil Microbial Diversity and Community to Facilitate Nitrogen Assimilation: A Potential Mechanism for Increasing Proso Millet Grain Yield |
title_sort |
intercropping alters the soil microbial diversity and community to facilitate nitrogen assimilation: a potential mechanism for increasing proso millet grain yield |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-11-01 |
description |
Intercropping of cereals and legumes has been used in modern agricultural systems, and the soil microorganisms associated with legumes play a vital role in organic matter decomposition and nitrogen (N) fixation. This study investigated the effect of intercropping on the rhizosphere soil microbial composition and structure and how this interaction affects N absorption and utilization by plants to improve crop productivity. Experiments were conducted to analyze the rhizosphere soil microbial diversity and the relationship between microbial composition and N assimilation by proso millet (Panicum miliaceum L.) and mung bean (Vigna radiata L.) from 2017 to 2019. Four different intercropping row arrangements were evaluated, and individual plantings of proso millet and mung bean were used as controls. Microbial diversity and community composition were determined through Illumina sequencing of 16S rRNA and internal transcribed spacer (ITS) genes. The results indicated that intercropping increased N levels in the soil–plant system and this alteration was strongly dependent on changes in the microbial (bacterial and fungal) diversities and communities. The increase in bacterial alpha diversity and changes in unique operational taxonomic unit (OTU) numbers increased the soil N availability and plant N accumulation. Certain bacterial taxa (such as Proteobacteria) and fungal taxa (such as Ascomycota) were significantly altered under intercropping and showed positive responses to increased N assimilation. The average grain yield of intercropped proso millet increased by 13.9–50.1% compared to that of monoculture proso millet. Our data clearly showed that intercropping proso millet with mung bean altered the rhizosphere soil microbial diversity and community composition; thus, this intercropping system represents a potential mechanism for promoting N assimilation and increasing grain yield. |
topic |
cereal–legume intercropping soil microbial community soil–plant system N availability grain yield |
url |
https://www.frontiersin.org/articles/10.3389/fmicb.2020.601054/full |
work_keys_str_mv |
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