The morphology of Ganoderma lucidum mycelium in a repeated-batch fermentation for exopolysaccharide production
The morphology of Ganoderma lucidum BCCM 31549 mycelium in a repeated-batch fermentation (RBF) was studied for exopolysaccharide (EPS) production. RBF was optimised for time to replace and volume to replace. G. lucidum mycelium showed the ability to self-immobilise and exhibited high stability for r...
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doaj-4e9c77ca6cb243a7b0f8bbbfd26957d52020-11-24T22:26:23ZengElsevierBiotechnology Reports2215-017X2016-09-0111C21110.1016/j.btre.2016.05.005The morphology of Ganoderma lucidum mycelium in a repeated-batch fermentation for exopolysaccharide productionWan Abd Al Qadr Imad Wan-Mohtar0Safuan Ab Kadir1Nazamid Saari2Fermentation Centre, SIPBS, HW429, John Arburthnott Building (Hamnett Wing), 161 Cathedral Street, University of Strathclyde, Glasgow, G4 0RE, Scotland, UKDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, MalaysiaThe morphology of Ganoderma lucidum BCCM 31549 mycelium in a repeated-batch fermentation (RBF) was studied for exopolysaccharide (EPS) production. RBF was optimised for time to replace and volume to replace. G. lucidum mycelium showed the ability to self-immobilise and exhibited high stability for repeated use in RBF with engulfed pellets. Furthermore, the ovoid and starburst-like pellet morphology was disposed to EPS production in the shake flask and bioreactor, respectively. Seven RBF could be carried out in 500 mL flasks, and five repeated batches were performed in a 2 L bioreactor. Under RBF conditions, autolysis of pellet core in the shake flask and shaving off of the outer hairy region in the bioreactor were observed at the later stages of RBF (R4 for the shake flask and R6 for the bioreactor). The proposed strategy showed that the morphology of G. lucidum mycelium can withstand extended fermentation cycles.http://www.sciencedirect.com/science/article/pii/S2215017X16300248Ganoderma lucidumMorphologyOvoid pelletStarburst-like pelletRepeated-batch fermentation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wan Abd Al Qadr Imad Wan-Mohtar Safuan Ab Kadir Nazamid Saari |
spellingShingle |
Wan Abd Al Qadr Imad Wan-Mohtar Safuan Ab Kadir Nazamid Saari The morphology of Ganoderma lucidum mycelium in a repeated-batch fermentation for exopolysaccharide production Biotechnology Reports Ganoderma lucidum Morphology Ovoid pellet Starburst-like pellet Repeated-batch fermentation |
author_facet |
Wan Abd Al Qadr Imad Wan-Mohtar Safuan Ab Kadir Nazamid Saari |
author_sort |
Wan Abd Al Qadr Imad Wan-Mohtar |
title |
The morphology of Ganoderma lucidum mycelium in a repeated-batch fermentation for exopolysaccharide production |
title_short |
The morphology of Ganoderma lucidum mycelium in a repeated-batch fermentation for exopolysaccharide production |
title_full |
The morphology of Ganoderma lucidum mycelium in a repeated-batch fermentation for exopolysaccharide production |
title_fullStr |
The morphology of Ganoderma lucidum mycelium in a repeated-batch fermentation for exopolysaccharide production |
title_full_unstemmed |
The morphology of Ganoderma lucidum mycelium in a repeated-batch fermentation for exopolysaccharide production |
title_sort |
morphology of ganoderma lucidum mycelium in a repeated-batch fermentation for exopolysaccharide production |
publisher |
Elsevier |
series |
Biotechnology Reports |
issn |
2215-017X |
publishDate |
2016-09-01 |
description |
The morphology of Ganoderma lucidum BCCM 31549 mycelium in a repeated-batch fermentation (RBF) was studied for exopolysaccharide (EPS) production. RBF was optimised for time to replace and volume to replace. G. lucidum mycelium showed the ability to self-immobilise and exhibited high stability for repeated use in RBF with engulfed pellets. Furthermore, the ovoid and starburst-like pellet morphology was disposed to EPS production in the shake flask and bioreactor, respectively. Seven RBF could be carried out in 500 mL flasks, and five repeated batches were performed in a 2 L bioreactor. Under RBF conditions, autolysis of pellet core in the shake flask and shaving off of the outer hairy region in the bioreactor were observed at the later stages of RBF (R4 for the shake flask and R6 for the bioreactor). The proposed strategy showed that the morphology of G. lucidum mycelium can withstand extended fermentation cycles. |
topic |
Ganoderma lucidum Morphology Ovoid pellet Starburst-like pellet Repeated-batch fermentation |
url |
http://www.sciencedirect.com/science/article/pii/S2215017X16300248 |
work_keys_str_mv |
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