Deconvolution of diffuse gastric cancer and the suppression of CD34 on the BALB/c nude mice model
Abstract Background Gastric cancer is a considerable burden for worldwide patients. And diffuse gastric cancer is the most insidious subgroup with poor survival. The phenotypic characterization of the diffuse gastric cancer cell line can be useful for gastric cancer researchers. In this article, we...
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doaj-4eb26c2c19dc4de5b257a8e706a7e1fe2020-11-25T03:07:27ZengBMCBMC Cancer1471-24072020-04-0120111010.1186/s12885-020-06814-4Deconvolution of diffuse gastric cancer and the suppression of CD34 on the BALB/c nude mice modelSeon-Jin Yoon0Jungmin Park1Youngmin Shin2Yuna Choi3Sahng Wook Park4Seok-Gu Kang5Hye Young Son6Yong-Min Huh7Department of Biochemistry and Molecular Biology, Yonsei University College of MedicineDepartment of Radiology, Severance Hospital, Yonsei University College of MedicineDepartment of Radiology, Severance Hospital, Yonsei University College of MedicineDepartment of Radiology, Severance Hospital, Yonsei University College of MedicineDepartment of Biochemistry and Molecular Biology, Yonsei University College of MedicineDepartments of Neurosurgery, Severance Hospital, Yonsei University College of MedicineSeverance Biomedical Science Institute, College of Medicine, Yonsei UniversityDepartment of Biochemistry and Molecular Biology, Yonsei University College of MedicineAbstract Background Gastric cancer is a considerable burden for worldwide patients. And diffuse gastric cancer is the most insidious subgroup with poor survival. The phenotypic characterization of the diffuse gastric cancer cell line can be useful for gastric cancer researchers. In this article, we aimed to characterize the diffuse gastric cancer cells with MRI and transcriptomic data. We hypothesized that gene expression pattern is associated with the phenotype of the cells and that the heterogeneous enhancement pattern and the high tumorigenicity of SNU484 can be modulated by the perturbation of the highly expressed gene. Methods We evaluated the 9.4 T magnetic resonance imaging and transcriptomic data of the orthotopic mice models from diffuse gastric cancer cells such as SNU484, Hs746T, SNU668, and KATO III. We included MKN74 as an intestinal cancer control cell. After comprehensive analysis integrating MRI and transcriptomic data, we selected CD34 and validated the effect by shRNA in the BALB/c nude mice models. Results SNU484, SNU668, Hs746T, and MKN74 formed orthotopic tumors by the 5 weeks after cell injection. The diffuse phenotype was found in the SNU484 and Hs746T. SNU484 was the only tumor showing the heterogeneous enhancement pattern on T2 images with a high level of CD34 expression. Knockdown of CD34 decreased the round-void shape in the H&E staining (P = 0.028), the heterogeneous T2 enhancement, and orthotopic tumorigenicity (100% vs 66.7%). The RNAseq showed that the suppressed CD34 is associated with the downregulated gene-sets of the extracellular matrix remodeling. Conclusion Suppression of CD34 in the human-originated gastric cancer cell suggests that it is important for the round-void histologic shape, heterogeneous enhancement pattern on MRI, and the growth of gastric cancer cell line.http://link.springer.com/article/10.1186/s12885-020-06814-4Diffuse gastric cancerCD34KnockdownMagnetic resonance imagingHistologyPhenotype |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Seon-Jin Yoon Jungmin Park Youngmin Shin Yuna Choi Sahng Wook Park Seok-Gu Kang Hye Young Son Yong-Min Huh |
spellingShingle |
Seon-Jin Yoon Jungmin Park Youngmin Shin Yuna Choi Sahng Wook Park Seok-Gu Kang Hye Young Son Yong-Min Huh Deconvolution of diffuse gastric cancer and the suppression of CD34 on the BALB/c nude mice model BMC Cancer Diffuse gastric cancer CD34 Knockdown Magnetic resonance imaging Histology Phenotype |
author_facet |
Seon-Jin Yoon Jungmin Park Youngmin Shin Yuna Choi Sahng Wook Park Seok-Gu Kang Hye Young Son Yong-Min Huh |
author_sort |
Seon-Jin Yoon |
title |
Deconvolution of diffuse gastric cancer and the suppression of CD34 on the BALB/c nude mice model |
title_short |
Deconvolution of diffuse gastric cancer and the suppression of CD34 on the BALB/c nude mice model |
title_full |
Deconvolution of diffuse gastric cancer and the suppression of CD34 on the BALB/c nude mice model |
title_fullStr |
Deconvolution of diffuse gastric cancer and the suppression of CD34 on the BALB/c nude mice model |
title_full_unstemmed |
Deconvolution of diffuse gastric cancer and the suppression of CD34 on the BALB/c nude mice model |
title_sort |
deconvolution of diffuse gastric cancer and the suppression of cd34 on the balb/c nude mice model |
publisher |
BMC |
series |
BMC Cancer |
issn |
1471-2407 |
publishDate |
2020-04-01 |
description |
Abstract Background Gastric cancer is a considerable burden for worldwide patients. And diffuse gastric cancer is the most insidious subgroup with poor survival. The phenotypic characterization of the diffuse gastric cancer cell line can be useful for gastric cancer researchers. In this article, we aimed to characterize the diffuse gastric cancer cells with MRI and transcriptomic data. We hypothesized that gene expression pattern is associated with the phenotype of the cells and that the heterogeneous enhancement pattern and the high tumorigenicity of SNU484 can be modulated by the perturbation of the highly expressed gene. Methods We evaluated the 9.4 T magnetic resonance imaging and transcriptomic data of the orthotopic mice models from diffuse gastric cancer cells such as SNU484, Hs746T, SNU668, and KATO III. We included MKN74 as an intestinal cancer control cell. After comprehensive analysis integrating MRI and transcriptomic data, we selected CD34 and validated the effect by shRNA in the BALB/c nude mice models. Results SNU484, SNU668, Hs746T, and MKN74 formed orthotopic tumors by the 5 weeks after cell injection. The diffuse phenotype was found in the SNU484 and Hs746T. SNU484 was the only tumor showing the heterogeneous enhancement pattern on T2 images with a high level of CD34 expression. Knockdown of CD34 decreased the round-void shape in the H&E staining (P = 0.028), the heterogeneous T2 enhancement, and orthotopic tumorigenicity (100% vs 66.7%). The RNAseq showed that the suppressed CD34 is associated with the downregulated gene-sets of the extracellular matrix remodeling. Conclusion Suppression of CD34 in the human-originated gastric cancer cell suggests that it is important for the round-void histologic shape, heterogeneous enhancement pattern on MRI, and the growth of gastric cancer cell line. |
topic |
Diffuse gastric cancer CD34 Knockdown Magnetic resonance imaging Histology Phenotype |
url |
http://link.springer.com/article/10.1186/s12885-020-06814-4 |
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