Phenotypic and molecular characterisations of carbapenem-resistant Acinetobacter baumannii strains isolated in Madagascar
Abstract Background The present study aimed to perform a deep phenotypic and genotypic analysis of 15 clinical carbapenem-resistant Acinetobacter baumannii (CRAb) strains isolated in Madagascar between 2008 and 2016 from diverse sources. Methods CRAb isolates collected from the Clinical Biology Cent...
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doaj-4eb56b5bcbac4bbeabe2e4564898733c2020-11-25T02:09:58ZengBMCAntimicrobial Resistance and Infection Control2047-29942019-02-01811910.1186/s13756-019-0491-9Phenotypic and molecular characterisations of carbapenem-resistant Acinetobacter baumannii strains isolated in MadagascarPierrette Landrie Simo Tchuinte0Mamitiana Alain Noah Rabenandrasana1Carole Kowalewicz2Volasoa Herilalaina Andrianoelina3Andriniaina Rakotondrasoa4Zafitsara Zo Andrianirina5Vincent Enouf6Elisoa Hariniaina Ratsima7Frédérique Randrianirina8Jean-Marc Collard9Institut Pasteur de MadagascarInstitut Pasteur de MadagascarInstitut Pasteur de MadagascarInstitut Pasteur de MadagascarInstitut Pasteur de MadagascarService de Pédiatrie et Néonatologie, Centre Hospitalier de SoavinandrianaInstitut Pasteur, Pasteur International Bioresources network (PIBnet), Plateforme de Microbiologie Mutualisée (P2M)Institut Pasteur de MadagascarInstitut Pasteur de MadagascarInstitut Pasteur de MadagascarAbstract Background The present study aimed to perform a deep phenotypic and genotypic analysis of 15 clinical carbapenem-resistant Acinetobacter baumannii (CRAb) strains isolated in Madagascar between 2008 and 2016 from diverse sources. Methods CRAb isolates collected from the Clinical Biology Centre of the Institut Pasteur of Madagascar, from the neonatal unit of Antananarivo military hospital, and from intensive care units of Mahajanga Androva and Antananarivo Joseph Ravoahangy Andrianavalona (HJRA) hospitals were subjected to susceptibility testing. Whole-genome sequencing allowed us to assess the presence of antibiotic-resistance determinants, insertion sequences, integrons, genomic islands and potential virulence factors in all strains. The structure of the carO porin gene and deduced protein (CarO) were also assessed in CRAb isolates. Results All isolates were found to be multidrug-resistant strains. Antibiotic-resistance genes against six classes of antimicrobial agents were described. The four carbapenem-resistance genes: bla OXA-51 like , bla OXA-23 , bla OXA-24 , and bla OXA-58 genes were detected in 100, 53.3, 13.3, and 6.6% of the isolates, respectively. Additionally, an ISAba1 located upstream of bla OXA-23 and bla ADC-like genes was observed in 53.3 and 66.7% of isolates, respectively. Further, Tn2006 and Tn2008 were found associated to the ISAba1-bla OXA-23 structure. An 8051-bp mobilizable plasmid harbouring the bla OXA-24 gene was isolated in two strains. In addition, 46.7% of isolates were positive for class 1 integrons. Overall, five sequences types (STs), with predominantly ST2, were detected. Several virulence genes were found in the CRAb isolates, among which two genes, epsA and ptk, responsible for the capsule-positive phenotype, were involved in A. baumannii pathogenesis. Conclusions This study revealed the presence of high-level carbapenem resistance in A. baumannii with the first description of OXA-24 and OXA-58 carbapenemases in Madagascar. This highlights the importance of better monitoring and controlling CRAb in Madagascan hospitals to avoid their spread.http://link.springer.com/article/10.1186/s13756-019-0491-9Acinetobacter baumanniiCarbapenem-resistanceAntimicrobial resistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pierrette Landrie Simo Tchuinte Mamitiana Alain Noah Rabenandrasana Carole Kowalewicz Volasoa Herilalaina Andrianoelina Andriniaina Rakotondrasoa Zafitsara Zo Andrianirina Vincent Enouf Elisoa Hariniaina Ratsima Frédérique Randrianirina Jean-Marc Collard |
spellingShingle |
Pierrette Landrie Simo Tchuinte Mamitiana Alain Noah Rabenandrasana Carole Kowalewicz Volasoa Herilalaina Andrianoelina Andriniaina Rakotondrasoa Zafitsara Zo Andrianirina Vincent Enouf Elisoa Hariniaina Ratsima Frédérique Randrianirina Jean-Marc Collard Phenotypic and molecular characterisations of carbapenem-resistant Acinetobacter baumannii strains isolated in Madagascar Antimicrobial Resistance and Infection Control Acinetobacter baumannii Carbapenem-resistance Antimicrobial resistance |
author_facet |
Pierrette Landrie Simo Tchuinte Mamitiana Alain Noah Rabenandrasana Carole Kowalewicz Volasoa Herilalaina Andrianoelina Andriniaina Rakotondrasoa Zafitsara Zo Andrianirina Vincent Enouf Elisoa Hariniaina Ratsima Frédérique Randrianirina Jean-Marc Collard |
author_sort |
Pierrette Landrie Simo Tchuinte |
title |
Phenotypic and molecular characterisations of carbapenem-resistant Acinetobacter baumannii strains isolated in Madagascar |
title_short |
Phenotypic and molecular characterisations of carbapenem-resistant Acinetobacter baumannii strains isolated in Madagascar |
title_full |
Phenotypic and molecular characterisations of carbapenem-resistant Acinetobacter baumannii strains isolated in Madagascar |
title_fullStr |
Phenotypic and molecular characterisations of carbapenem-resistant Acinetobacter baumannii strains isolated in Madagascar |
title_full_unstemmed |
Phenotypic and molecular characterisations of carbapenem-resistant Acinetobacter baumannii strains isolated in Madagascar |
title_sort |
phenotypic and molecular characterisations of carbapenem-resistant acinetobacter baumannii strains isolated in madagascar |
publisher |
BMC |
series |
Antimicrobial Resistance and Infection Control |
issn |
2047-2994 |
publishDate |
2019-02-01 |
description |
Abstract Background The present study aimed to perform a deep phenotypic and genotypic analysis of 15 clinical carbapenem-resistant Acinetobacter baumannii (CRAb) strains isolated in Madagascar between 2008 and 2016 from diverse sources. Methods CRAb isolates collected from the Clinical Biology Centre of the Institut Pasteur of Madagascar, from the neonatal unit of Antananarivo military hospital, and from intensive care units of Mahajanga Androva and Antananarivo Joseph Ravoahangy Andrianavalona (HJRA) hospitals were subjected to susceptibility testing. Whole-genome sequencing allowed us to assess the presence of antibiotic-resistance determinants, insertion sequences, integrons, genomic islands and potential virulence factors in all strains. The structure of the carO porin gene and deduced protein (CarO) were also assessed in CRAb isolates. Results All isolates were found to be multidrug-resistant strains. Antibiotic-resistance genes against six classes of antimicrobial agents were described. The four carbapenem-resistance genes: bla OXA-51 like , bla OXA-23 , bla OXA-24 , and bla OXA-58 genes were detected in 100, 53.3, 13.3, and 6.6% of the isolates, respectively. Additionally, an ISAba1 located upstream of bla OXA-23 and bla ADC-like genes was observed in 53.3 and 66.7% of isolates, respectively. Further, Tn2006 and Tn2008 were found associated to the ISAba1-bla OXA-23 structure. An 8051-bp mobilizable plasmid harbouring the bla OXA-24 gene was isolated in two strains. In addition, 46.7% of isolates were positive for class 1 integrons. Overall, five sequences types (STs), with predominantly ST2, were detected. Several virulence genes were found in the CRAb isolates, among which two genes, epsA and ptk, responsible for the capsule-positive phenotype, were involved in A. baumannii pathogenesis. Conclusions This study revealed the presence of high-level carbapenem resistance in A. baumannii with the first description of OXA-24 and OXA-58 carbapenemases in Madagascar. This highlights the importance of better monitoring and controlling CRAb in Madagascan hospitals to avoid their spread. |
topic |
Acinetobacter baumannii Carbapenem-resistance Antimicrobial resistance |
url |
http://link.springer.com/article/10.1186/s13756-019-0491-9 |
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