Different Spectrophotometric Methods for Simultaneous Determination of Trelagliptin and Its Acid Degradation Product
New spectrophotometric and chemometric methods were carried out for the simultaneous assay of trelagliptin (TRG) and its acid degradation product (TAD) and applied successfully as a stability indicating assay to recently approved Zafatek® tablets. TAD was monitored using TLC to ensure complete degra...
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2018-01-01
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Series: | Journal of Analytical Methods in Chemistry |
Online Access: | http://dx.doi.org/10.1155/2018/7370651 |
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doaj-4eb6b2a2cdd943f1ae44e692ea36ca862020-11-25T01:22:57ZengHindawi LimitedJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/73706517370651Different Spectrophotometric Methods for Simultaneous Determination of Trelagliptin and Its Acid Degradation ProductShereen Mowaka0Bassam M. Ayoub1Mostafa A. Hassan2Wafaa A. Zaghary3Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Ein Helwan, Cairo 11795, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt, El-Shorouk City, Cairo 11837, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt, El-Shorouk City, Cairo 11837, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Helwan University, Ein Helwan, Cairo 11795, EgyptNew spectrophotometric and chemometric methods were carried out for the simultaneous assay of trelagliptin (TRG) and its acid degradation product (TAD) and applied successfully as a stability indicating assay to recently approved Zafatek® tablets. TAD was monitored using TLC to ensure complete degradation. Furthermore, HPLC was used to confirm dealing with one major acid degradation product. The proposed methods were developed by manipulating zero-order, first-derivative, and ratio spectra of TRG and TAD using simultaneous equation, first-derivative, and mean-centering methods, respectively. Using Spectra Manager II and Minitab v.14 software, the absorbance at 274 nm–260.4 nm, amplitudes at 260.4 nm–274.0 nm, and mean-centered values at 287.6 nm–257.2 nm were measured against methanol as a blank for TRG and TAD, respectively. Linearity and the other validation parameters were acceptable at concentration ranges of 5–50 μg/mL and 2.5–25 μg/mL for TRG and TAD, respectively. Using one-way analysis of variance (ANOVA), the optimized methods were compared and proved to be accurate for the simultaneous assay of TRG and TAD.http://dx.doi.org/10.1155/2018/7370651 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shereen Mowaka Bassam M. Ayoub Mostafa A. Hassan Wafaa A. Zaghary |
spellingShingle |
Shereen Mowaka Bassam M. Ayoub Mostafa A. Hassan Wafaa A. Zaghary Different Spectrophotometric Methods for Simultaneous Determination of Trelagliptin and Its Acid Degradation Product Journal of Analytical Methods in Chemistry |
author_facet |
Shereen Mowaka Bassam M. Ayoub Mostafa A. Hassan Wafaa A. Zaghary |
author_sort |
Shereen Mowaka |
title |
Different Spectrophotometric Methods for Simultaneous Determination of Trelagliptin and Its Acid Degradation Product |
title_short |
Different Spectrophotometric Methods for Simultaneous Determination of Trelagliptin and Its Acid Degradation Product |
title_full |
Different Spectrophotometric Methods for Simultaneous Determination of Trelagliptin and Its Acid Degradation Product |
title_fullStr |
Different Spectrophotometric Methods for Simultaneous Determination of Trelagliptin and Its Acid Degradation Product |
title_full_unstemmed |
Different Spectrophotometric Methods for Simultaneous Determination of Trelagliptin and Its Acid Degradation Product |
title_sort |
different spectrophotometric methods for simultaneous determination of trelagliptin and its acid degradation product |
publisher |
Hindawi Limited |
series |
Journal of Analytical Methods in Chemistry |
issn |
2090-8865 2090-8873 |
publishDate |
2018-01-01 |
description |
New spectrophotometric and chemometric methods were carried out for the simultaneous assay of trelagliptin (TRG) and its acid degradation product (TAD) and applied successfully as a stability indicating assay to recently approved Zafatek® tablets. TAD was monitored using TLC to ensure complete degradation. Furthermore, HPLC was used to confirm dealing with one major acid degradation product. The proposed methods were developed by manipulating zero-order, first-derivative, and ratio spectra of TRG and TAD using simultaneous equation, first-derivative, and mean-centering methods, respectively. Using Spectra Manager II and Minitab v.14 software, the absorbance at 274 nm–260.4 nm, amplitudes at 260.4 nm–274.0 nm, and mean-centered values at 287.6 nm–257.2 nm were measured against methanol as a blank for TRG and TAD, respectively. Linearity and the other validation parameters were acceptable at concentration ranges of 5–50 μg/mL and 2.5–25 μg/mL for TRG and TAD, respectively. Using one-way analysis of variance (ANOVA), the optimized methods were compared and proved to be accurate for the simultaneous assay of TRG and TAD. |
url |
http://dx.doi.org/10.1155/2018/7370651 |
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