Transcription Factor SmWRKY1 Positively Promotes the Biosynthesis of Tanshinones in Salvia miltiorrhiza
Tanshinones, one group of bioactive diterpenes, were widely used in the treatment of cardiovascular diseases. WRKYs play important roles in plant metabolism, but their regulation mechanism in Salvia miltiorrhiza remains elusive. In this study, one WRKY transcription factor SmWRKY1 was isolated and f...
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doaj-4ec8e1eb1c2f472caa6dab7ead11dc5d2020-11-24T23:59:33ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-04-01910.3389/fpls.2018.00554341721Transcription Factor SmWRKY1 Positively Promotes the Biosynthesis of Tanshinones in Salvia miltiorrhizaWenzhi Cao0Yao Wang1Min Shi2Xiaolong Hao3Weiwei Zhao4Yu Wang5Jie Ren6Guoyin Kai7Guoyin Kai8Institute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, ChinaInstitute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, ChinaInstitute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, ChinaInstitute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, ChinaInstitute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, ChinaInstitute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, ChinaInstitute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, ChinaInstitute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, ChinaCollege of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, ChinaTanshinones, one group of bioactive diterpenes, were widely used in the treatment of cardiovascular diseases. WRKYs play important roles in plant metabolism, but their regulation mechanism in Salvia miltiorrhiza remains elusive. In this study, one WRKY transcription factor SmWRKY1 was isolated and functionally characterized from S. miltiorrhiza. Multiple sequence alignment and phylogenetic tree analysis showed SmWRKY1 shared high homology with other plant WRKYs such as CrWRKY1. SmWRKY1 was found predominantly expressed in leaves and stems, and was responsive to salicylic acid (SA), methyl jasmonate (MeJA), and nitric oxide (NO) treatment. Subcellular localization analysis found that SmWRKY1 was localized in the nucleus. Over-expression of SmWRKY1 significantly elevated the transcripts of genes coding for enzymes in the MEP pathway especially 1-deoxy-D-xylulose-5-phosphate synthase (SmDXS) and 1-deoxy-D-xylulose-5-phosphate reductoisomerase (SmDXR), resulted in over fivefold increase in tanshinones production in transgenic lines (up to 13.7 mg/g DW) compared with the control lines. A dual-luciferase (Dual-LUC) assay showed that SmWRKY1 can positively regulate SmDXR expression by binding to its promoter. Our work revealed that SmWRKY1 participated in the regulation of tanshinones biosynthesis and acted as a positive regulator through activating SmDXR in the MEP pathway, thus provided a new insight to further explore the regulation mechanism of tanshinones biosynthesis.http://journal.frontiersin.org/article/10.3389/fpls.2018.00554/fullSalvia miltiorrhizahairy rootsSmWRKY1MEP pathwaytanshinonesmetabolic engineering |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wenzhi Cao Yao Wang Min Shi Xiaolong Hao Weiwei Zhao Yu Wang Jie Ren Guoyin Kai Guoyin Kai |
spellingShingle |
Wenzhi Cao Yao Wang Min Shi Xiaolong Hao Weiwei Zhao Yu Wang Jie Ren Guoyin Kai Guoyin Kai Transcription Factor SmWRKY1 Positively Promotes the Biosynthesis of Tanshinones in Salvia miltiorrhiza Frontiers in Plant Science Salvia miltiorrhiza hairy roots SmWRKY1 MEP pathway tanshinones metabolic engineering |
author_facet |
Wenzhi Cao Yao Wang Min Shi Xiaolong Hao Weiwei Zhao Yu Wang Jie Ren Guoyin Kai Guoyin Kai |
author_sort |
Wenzhi Cao |
title |
Transcription Factor SmWRKY1 Positively Promotes the Biosynthesis of Tanshinones in Salvia miltiorrhiza |
title_short |
Transcription Factor SmWRKY1 Positively Promotes the Biosynthesis of Tanshinones in Salvia miltiorrhiza |
title_full |
Transcription Factor SmWRKY1 Positively Promotes the Biosynthesis of Tanshinones in Salvia miltiorrhiza |
title_fullStr |
Transcription Factor SmWRKY1 Positively Promotes the Biosynthesis of Tanshinones in Salvia miltiorrhiza |
title_full_unstemmed |
Transcription Factor SmWRKY1 Positively Promotes the Biosynthesis of Tanshinones in Salvia miltiorrhiza |
title_sort |
transcription factor smwrky1 positively promotes the biosynthesis of tanshinones in salvia miltiorrhiza |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2018-04-01 |
description |
Tanshinones, one group of bioactive diterpenes, were widely used in the treatment of cardiovascular diseases. WRKYs play important roles in plant metabolism, but their regulation mechanism in Salvia miltiorrhiza remains elusive. In this study, one WRKY transcription factor SmWRKY1 was isolated and functionally characterized from S. miltiorrhiza. Multiple sequence alignment and phylogenetic tree analysis showed SmWRKY1 shared high homology with other plant WRKYs such as CrWRKY1. SmWRKY1 was found predominantly expressed in leaves and stems, and was responsive to salicylic acid (SA), methyl jasmonate (MeJA), and nitric oxide (NO) treatment. Subcellular localization analysis found that SmWRKY1 was localized in the nucleus. Over-expression of SmWRKY1 significantly elevated the transcripts of genes coding for enzymes in the MEP pathway especially 1-deoxy-D-xylulose-5-phosphate synthase (SmDXS) and 1-deoxy-D-xylulose-5-phosphate reductoisomerase (SmDXR), resulted in over fivefold increase in tanshinones production in transgenic lines (up to 13.7 mg/g DW) compared with the control lines. A dual-luciferase (Dual-LUC) assay showed that SmWRKY1 can positively regulate SmDXR expression by binding to its promoter. Our work revealed that SmWRKY1 participated in the regulation of tanshinones biosynthesis and acted as a positive regulator through activating SmDXR in the MEP pathway, thus provided a new insight to further explore the regulation mechanism of tanshinones biosynthesis. |
topic |
Salvia miltiorrhiza hairy roots SmWRKY1 MEP pathway tanshinones metabolic engineering |
url |
http://journal.frontiersin.org/article/10.3389/fpls.2018.00554/full |
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