Two-dimensional thin-layer chromatography of rat liver phosphatides

Two-dimensional thin-layer chromatography of rat liver phosphatides

A system of two-dimensional thin-layer chromatography was developed that separated rat liver phosphatides into several phosphate-positive spots in about 2 hr developing time. Characteristic hydrolysis products derived from phosphatidyl serine, phosphatidyl ethanolamine, phosphatidyl inositol, phosph...

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Bibliographic Details
Main Authors: W.D. Skidmore, C. Entenman
Format: Article
Language:English
Published: Elsevier 1962-10-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520403943
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spelling doaj-4f0ac866a8ac4cc5b352dfaf287916882021-04-23T06:12:55ZengElsevierJournal of Lipid Research0022-22751962-10-0134471475Two-dimensional thin-layer chromatography of rat liver phosphatidesW.D. Skidmore0C. Entenman1U. S. Naval Radiological Defense Laboratory, San Francisco, CaliforniaU. S. Naval Radiological Defense Laboratory, San Francisco, CaliforniaA system of two-dimensional thin-layer chromatography was developed that separated rat liver phosphatides into several phosphate-positive spots in about 2 hr developing time. Characteristic hydrolysis products derived from phosphatidyl serine, phosphatidyl ethanolamine, phosphatidyl inositol, phosphatidyl choline, sphingomyelin, and lysophosphatidyl choline were identified. The hydrolytic products of “phosphatidic acid” were not definitely characterized. The application of thin-layer chromatography as described for rat liver phosphatides can be extended to phosphatide extracts of other tissues.http://www.sciencedirect.com/science/article/pii/S0022227520403943
collection DOAJ
language English
format Article
sources DOAJ
author W.D. Skidmore
C. Entenman
spellingShingle W.D. Skidmore
C. Entenman
Two-dimensional thin-layer chromatography of rat liver phosphatides
Journal of Lipid Research
author_facet W.D. Skidmore
C. Entenman
author_sort W.D. Skidmore
title Two-dimensional thin-layer chromatography of rat liver phosphatides
title_short Two-dimensional thin-layer chromatography of rat liver phosphatides
title_full Two-dimensional thin-layer chromatography of rat liver phosphatides
title_fullStr Two-dimensional thin-layer chromatography of rat liver phosphatides
title_full_unstemmed Two-dimensional thin-layer chromatography of rat liver phosphatides
title_sort two-dimensional thin-layer chromatography of rat liver phosphatides
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1962-10-01
description A system of two-dimensional thin-layer chromatography was developed that separated rat liver phosphatides into several phosphate-positive spots in about 2 hr developing time. Characteristic hydrolysis products derived from phosphatidyl serine, phosphatidyl ethanolamine, phosphatidyl inositol, phosphatidyl choline, sphingomyelin, and lysophosphatidyl choline were identified. The hydrolytic products of “phosphatidic acid” were not definitely characterized. The application of thin-layer chromatography as described for rat liver phosphatides can be extended to phosphatide extracts of other tissues.
url http://www.sciencedirect.com/science/article/pii/S0022227520403943
work_keys_str_mv AT wdskidmore twodimensionalthinlayerchromatographyofratliverphosphatides
AT centenman twodimensionalthinlayerchromatographyofratliverphosphatides
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