Kinetics Extraction Modelling and Antiproliferative Activity of Clinacanthus nutans Water Extract
Clinacanthus nutans is widely grown in tropical Asia and locally known “belalai gajah” or Sabah snake grass. It has been used as a natural product to treat skin rashes, snake bites, lesion caused by herpes, diabetes, fever, and cancer. Therefore, the objectives of this research are to determine the...
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doaj-4f19c0ca1c014b56b9bea851fe3f80932020-11-25T00:15:26ZengHindawi LimitedThe Scientific World Journal2356-61401537-744X2016-01-01201610.1155/2016/73705367370536Kinetics Extraction Modelling and Antiproliferative Activity of Clinacanthus nutans Water ExtractFarah Nadiah Mohd Fazil0Nur Syarafina Mohd Azzimi1Badrul Hisham Yahaya2Nurulain Atikah Kamalaldin3Saiful Irwan Zubairi4School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, MalaysiaSchool of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, MalaysiaAdvanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia (USM), Bertam, 13200 Kepala Batas, Pulau Pinang, MalaysiaAdvanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia (USM), Bertam, 13200 Kepala Batas, Pulau Pinang, MalaysiaSchool of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, MalaysiaClinacanthus nutans is widely grown in tropical Asia and locally known “belalai gajah” or Sabah snake grass. It has been used as a natural product to treat skin rashes, snake bites, lesion caused by herpes, diabetes, fever, and cancer. Therefore, the objectives of this research are to determine the maximum yield and time of exhaustive flavonoids extraction using Peleg’s model and to evaluate potential of antiproliferative activity on human lung cancer cell (A549). The extraction process was carried out on fresh and dried leaves at 28 to 30°C with liquid-to-solid ratio of 10 mL/g for 72 hrs. The extracts were collected intermittently analysed using mathematical Peleg’s model and RP-HPLC. The highest amount of flavonoids was used to evaluate the inhibitory concentration (IC50) via 2D cell culture of A549. Based on the results obtained, the predicted maximum extract density was observed at 29.20 ± 14.54 hrs of extraction (texhaustive). However, the exhaustive time of extraction to acquire maximum flavonoids content exhibited approximately 10 hrs earlier. Therefore, 18 hrs of extraction time was chosen to acquire high content of flavonoids. The best antiproliferative effect (IC50) on A549 cell line was observed at 138.82 ± 0.60 µg/mL. In conclusion, the flavonoids content in Clinacanthus nutans water extract possesses potential antiproliferative properties against A549, suggesting an alternative approach for cancer treatment.http://dx.doi.org/10.1155/2016/7370536 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Farah Nadiah Mohd Fazil Nur Syarafina Mohd Azzimi Badrul Hisham Yahaya Nurulain Atikah Kamalaldin Saiful Irwan Zubairi |
spellingShingle |
Farah Nadiah Mohd Fazil Nur Syarafina Mohd Azzimi Badrul Hisham Yahaya Nurulain Atikah Kamalaldin Saiful Irwan Zubairi Kinetics Extraction Modelling and Antiproliferative Activity of Clinacanthus nutans Water Extract The Scientific World Journal |
author_facet |
Farah Nadiah Mohd Fazil Nur Syarafina Mohd Azzimi Badrul Hisham Yahaya Nurulain Atikah Kamalaldin Saiful Irwan Zubairi |
author_sort |
Farah Nadiah Mohd Fazil |
title |
Kinetics Extraction Modelling and Antiproliferative Activity of Clinacanthus nutans Water Extract |
title_short |
Kinetics Extraction Modelling and Antiproliferative Activity of Clinacanthus nutans Water Extract |
title_full |
Kinetics Extraction Modelling and Antiproliferative Activity of Clinacanthus nutans Water Extract |
title_fullStr |
Kinetics Extraction Modelling and Antiproliferative Activity of Clinacanthus nutans Water Extract |
title_full_unstemmed |
Kinetics Extraction Modelling and Antiproliferative Activity of Clinacanthus nutans Water Extract |
title_sort |
kinetics extraction modelling and antiproliferative activity of clinacanthus nutans water extract |
publisher |
Hindawi Limited |
series |
The Scientific World Journal |
issn |
2356-6140 1537-744X |
publishDate |
2016-01-01 |
description |
Clinacanthus nutans is widely grown in tropical Asia and locally known “belalai gajah” or Sabah snake grass. It has been used as a natural product to treat skin rashes, snake bites, lesion caused by herpes, diabetes, fever, and cancer. Therefore, the objectives of this research are to determine the maximum yield and time of exhaustive flavonoids extraction using Peleg’s model and to evaluate potential of antiproliferative activity on human lung cancer cell (A549). The extraction process was carried out on fresh and dried leaves at 28 to 30°C with liquid-to-solid ratio of 10 mL/g for 72 hrs. The extracts were collected intermittently analysed using mathematical Peleg’s model and RP-HPLC. The highest amount of flavonoids was used to evaluate the inhibitory concentration (IC50) via 2D cell culture of A549. Based on the results obtained, the predicted maximum extract density was observed at 29.20 ± 14.54 hrs of extraction (texhaustive). However, the exhaustive time of extraction to acquire maximum flavonoids content exhibited approximately 10 hrs earlier. Therefore, 18 hrs of extraction time was chosen to acquire high content of flavonoids. The best antiproliferative effect (IC50) on A549 cell line was observed at 138.82 ± 0.60 µg/mL. In conclusion, the flavonoids content in Clinacanthus nutans water extract possesses potential antiproliferative properties against A549, suggesting an alternative approach for cancer treatment. |
url |
http://dx.doi.org/10.1155/2016/7370536 |
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