Red Light Controls Adventitious Root Regeneration by Modulating Hormone Homeostasis in Picea abies Seedlings
Vegetative propagation relies on the capacity of plants to regenerate de novo adventitious roots (ARs), a quantitative trait controlled by the interaction of endogenous factors, such as hormones and environmental cues among which light plays a central role. However, the physiological and molecular c...
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doaj-4f2e83bc9f884bda952fd1b4ed1029052020-11-25T03:37:42ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-09-011110.3389/fpls.2020.586140586140Red Light Controls Adventitious Root Regeneration by Modulating Hormone Homeostasis in Picea abies SeedlingsSanaria Alallaq0Sanaria Alallaq1Alok Ranjan2Federica Brunoni3Ondřej Novák4Ondřej Novák5Abdellah Lakehal6Catherine Bellini7Catherine Bellini8Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, SwedenDepartment of Biology, College of Science for Women, Baghdad University, Baghdad, IraqUmeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, SwedenUmeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, SwedenUmeå Plant Science Centre, Department of Forest Genetics and Physiology, Swedish Agriculture University, Umea, SwedenLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, The Czech Academy of Sciences, Olomouc, CzechiaUmeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, SwedenUmeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, SwedenInstitut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, Versailles, FranceVegetative propagation relies on the capacity of plants to regenerate de novo adventitious roots (ARs), a quantitative trait controlled by the interaction of endogenous factors, such as hormones and environmental cues among which light plays a central role. However, the physiological and molecular components mediating light cues during AR initiation (ARI) remain largely elusive. Here, we explored the role of red light (RL) on ARI in de-rooted Norway spruce seedlings. We combined investigation of hormone metabolism and gene expression analysis to identify potential signaling pathways. We also performed extensive anatomical characterization to investigate ARI at the cellular level. We showed that in contrast to white light, red light promoted ARI likely by reducing jasmonate (JA) and JA-isoleucine biosynthesis and repressing the accumulation of isopentyl-adenine-type cytokinins. We demonstrated that exogenously applied JA and/or CK inhibit ARI in a dose-dependent manner and found that they possibly act in the same pathway. The negative effect of JA on ARI was confirmed at the histological level. We showed that JA represses the early events of ARI. In conclusion, RL promotes ARI by repressing the accumulation of the wound-induced phytohormones JA and CK.https://www.frontiersin.org/article/10.3389/fpls.2020.586140/fulladventitious rootsconifersPicea abiesauxincytokininsjasmonate |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sanaria Alallaq Sanaria Alallaq Alok Ranjan Federica Brunoni Ondřej Novák Ondřej Novák Abdellah Lakehal Catherine Bellini Catherine Bellini |
spellingShingle |
Sanaria Alallaq Sanaria Alallaq Alok Ranjan Federica Brunoni Ondřej Novák Ondřej Novák Abdellah Lakehal Catherine Bellini Catherine Bellini Red Light Controls Adventitious Root Regeneration by Modulating Hormone Homeostasis in Picea abies Seedlings Frontiers in Plant Science adventitious roots conifers Picea abies auxin cytokinins jasmonate |
author_facet |
Sanaria Alallaq Sanaria Alallaq Alok Ranjan Federica Brunoni Ondřej Novák Ondřej Novák Abdellah Lakehal Catherine Bellini Catherine Bellini |
author_sort |
Sanaria Alallaq |
title |
Red Light Controls Adventitious Root Regeneration by Modulating Hormone Homeostasis in Picea abies Seedlings |
title_short |
Red Light Controls Adventitious Root Regeneration by Modulating Hormone Homeostasis in Picea abies Seedlings |
title_full |
Red Light Controls Adventitious Root Regeneration by Modulating Hormone Homeostasis in Picea abies Seedlings |
title_fullStr |
Red Light Controls Adventitious Root Regeneration by Modulating Hormone Homeostasis in Picea abies Seedlings |
title_full_unstemmed |
Red Light Controls Adventitious Root Regeneration by Modulating Hormone Homeostasis in Picea abies Seedlings |
title_sort |
red light controls adventitious root regeneration by modulating hormone homeostasis in picea abies seedlings |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2020-09-01 |
description |
Vegetative propagation relies on the capacity of plants to regenerate de novo adventitious roots (ARs), a quantitative trait controlled by the interaction of endogenous factors, such as hormones and environmental cues among which light plays a central role. However, the physiological and molecular components mediating light cues during AR initiation (ARI) remain largely elusive. Here, we explored the role of red light (RL) on ARI in de-rooted Norway spruce seedlings. We combined investigation of hormone metabolism and gene expression analysis to identify potential signaling pathways. We also performed extensive anatomical characterization to investigate ARI at the cellular level. We showed that in contrast to white light, red light promoted ARI likely by reducing jasmonate (JA) and JA-isoleucine biosynthesis and repressing the accumulation of isopentyl-adenine-type cytokinins. We demonstrated that exogenously applied JA and/or CK inhibit ARI in a dose-dependent manner and found that they possibly act in the same pathway. The negative effect of JA on ARI was confirmed at the histological level. We showed that JA represses the early events of ARI. In conclusion, RL promotes ARI by repressing the accumulation of the wound-induced phytohormones JA and CK. |
topic |
adventitious roots conifers Picea abies auxin cytokinins jasmonate |
url |
https://www.frontiersin.org/article/10.3389/fpls.2020.586140/full |
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