Expression of miR⁃21, miR⁃221, and miR⁃222 in exosomes of CAL27 tongue squamous cell carcinoma cells
Objective To assess the expression of miR ⁃21, miR ⁃221, and miR ⁃222 in exosomes of CAL27 tongue squamous cell carcinoma cells. Methods CAL27 tongue squamous cell carcinoma cells and normal human oral kerati⁃ nocytes (HOKs) were cultured, and then, the cultured supernatant was collected to separa...
Main Authors: | , , , |
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Format: | Article |
Language: | zho |
Published: |
Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
2018-04-01
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Series: | 口腔疾病防治 |
Subjects: | |
Online Access: | http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2018.04.004 |
Summary: | Objective To assess the expression of miR ⁃21, miR ⁃221, and miR ⁃222 in exosomes of CAL27 tongue
squamous cell carcinoma cells. Methods CAL27 tongue squamous cell carcinoma cells and normal human oral kerati⁃
nocytes (HOKs) were cultured, and then, the cultured supernatant was collected to separate the exosomes. Exosomes were
detected by electron microscopy, and the expression levels of miR⁃21, miR⁃221, and miR⁃222 in the exosomes of tongue
cancer cells were measured by qRT⁃PCR. Results Exosomes existed in the cultured supernatants of CAL27 cells and
HOKs. Additionally, the expression levels of miR⁃21, miR⁃221, and miR⁃222 in the exosomes of CAL27 cells were signifi⁃
cantly enhanced compared with those in the HOK exosomes (P < 0.05). Conclusion The expression levels of miR⁃21,
miR⁃221, and miR⁃222 were markedly enhanced in the exosomes of CAL27 tongue squamous cell carcinoma cells. |
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ISSN: | 2096-1456 2096-1456 |