Carnosine Protects Mouse Podocytes from High Glucose Induced Apoptosis through PI3K/AKT and Nrf2 Pathways
Diabetic nephropathy is the complication of diabetes mellitus that can lead to chronic renal failure. Reactive oxygen species (ROS) production plays an important role in its pathological process. Previous studies showed that carnosine may reduce diabetic nephropathy by antioxidant effect. However, t...
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Online Access: | http://dx.doi.org/10.1155/2019/4348973 |
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doaj-4f3b037da58b447382dab06c100c608e2020-11-25T01:08:09ZengHindawi LimitedBioMed Research International2314-61332314-61412019-01-01201910.1155/2019/43489734348973Carnosine Protects Mouse Podocytes from High Glucose Induced Apoptosis through PI3K/AKT and Nrf2 PathwaysKunxiao Zhao0Ying Li1Ziqiang Wang2Ning Han3Ying Wang4Department of Nephrology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei Province 050051, ChinaDepartment of Nephrology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei Province 050051, ChinaDepartment of Nephrology, Cangzhou People's Hospital, Cangzhou, Hebei Province 061000, ChinaDepartment of Nephrology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei Province 050051, ChinaDepartment of Nephrology, BayanNur Hospital, Bayan Nur, Inner Mongolia Autonomous Region 015000, ChinaDiabetic nephropathy is the complication of diabetes mellitus that can lead to chronic renal failure. Reactive oxygen species (ROS) production plays an important role in its pathological process. Previous studies showed that carnosine may reduce diabetic nephropathy by antioxidant effect. However, the molecular mechanism of its antioxidant was not fully understood. In the current study, we developed high glucose containing different concentrations of carnosine to reduce ROS levels and podocytes apoptosis, and Cell Counting Kit-8 test was used to observe the cell viability. Carnosine (5-20mM) was found to protect mouse podocytes (MPC5) cells from HG-induced injury. Quantitative real-time PCR, Western blotting, and immunofluorescence staining revealed that high glucose induced ROS levels and podocytes apoptosis were downregulated by PI3K/AKT and Nrf2 signaling pathways. The current findings suggest that carnosine may reduce ROS levels and MPC5 cells apoptosis by PI3K/AKT and Nrf2 signaling pathways activation.http://dx.doi.org/10.1155/2019/4348973 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kunxiao Zhao Ying Li Ziqiang Wang Ning Han Ying Wang |
spellingShingle |
Kunxiao Zhao Ying Li Ziqiang Wang Ning Han Ying Wang Carnosine Protects Mouse Podocytes from High Glucose Induced Apoptosis through PI3K/AKT and Nrf2 Pathways BioMed Research International |
author_facet |
Kunxiao Zhao Ying Li Ziqiang Wang Ning Han Ying Wang |
author_sort |
Kunxiao Zhao |
title |
Carnosine Protects Mouse Podocytes from High Glucose Induced Apoptosis through PI3K/AKT and Nrf2 Pathways |
title_short |
Carnosine Protects Mouse Podocytes from High Glucose Induced Apoptosis through PI3K/AKT and Nrf2 Pathways |
title_full |
Carnosine Protects Mouse Podocytes from High Glucose Induced Apoptosis through PI3K/AKT and Nrf2 Pathways |
title_fullStr |
Carnosine Protects Mouse Podocytes from High Glucose Induced Apoptosis through PI3K/AKT and Nrf2 Pathways |
title_full_unstemmed |
Carnosine Protects Mouse Podocytes from High Glucose Induced Apoptosis through PI3K/AKT and Nrf2 Pathways |
title_sort |
carnosine protects mouse podocytes from high glucose induced apoptosis through pi3k/akt and nrf2 pathways |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2019-01-01 |
description |
Diabetic nephropathy is the complication of diabetes mellitus that can lead to chronic renal failure. Reactive oxygen species (ROS) production plays an important role in its pathological process. Previous studies showed that carnosine may reduce diabetic nephropathy by antioxidant effect. However, the molecular mechanism of its antioxidant was not fully understood. In the current study, we developed high glucose containing different concentrations of carnosine to reduce ROS levels and podocytes apoptosis, and Cell Counting Kit-8 test was used to observe the cell viability. Carnosine (5-20mM) was found to protect mouse podocytes (MPC5) cells from HG-induced injury. Quantitative real-time PCR, Western blotting, and immunofluorescence staining revealed that high glucose induced ROS levels and podocytes apoptosis were downregulated by PI3K/AKT and Nrf2 signaling pathways. The current findings suggest that carnosine may reduce ROS levels and MPC5 cells apoptosis by PI3K/AKT and Nrf2 signaling pathways activation. |
url |
http://dx.doi.org/10.1155/2019/4348973 |
work_keys_str_mv |
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1725184021746417664 |