Expression and Activity of Lysozyme in Apis Mellifera Carnica Brood Infested with Varroa Destructor
Varroa destructor is a parasitic mite that attacks the honey bee, and previous studies have suggested that parasitosis caused by this mite is accompanied by immunosuppresion in the host. In this study, the effect of mite infestation on the expression of the lysozyme-1 (lys-1) gene and lysozyme activ...
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doaj-4f3b8d9e37a949e4813673c59af9e1892021-09-06T19:40:25ZengSciendoJournal of Apicultural Science2299-48312017-12-0161225325610.1515/jas-2017-0014jas-2017-0014Expression and Activity of Lysozyme in Apis Mellifera Carnica Brood Infested with Varroa DestructorZaobidna Ewa A.0Żółtowska Krystyna1Łopieńska-Biernat Elżbieta2Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury, Olsztyn, PolandDepartment of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury, Olsztyn, PolandDepartment of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury, Olsztyn, PolandVarroa destructor is a parasitic mite that attacks the honey bee, and previous studies have suggested that parasitosis caused by this mite is accompanied by immunosuppresion in the host. In this study, the effect of mite infestation on the expression of the lysozyme-1 (lys-1) gene and lysozyme activity in Apis mellifera carnica was determined. The experiment was carried out on the five developmental stages of honey bee workers and drones. Developmental and gender-related differences in gene expression and lysozyme activity were observed in a Varroa destructor-infested brood. The relative expression of the lys-1 gene increased in a infested worker brood and decreased in a drone brood except for P3 pupae. In the final stage of development, the lys-1 gene expression was significantly lower in infested newly emerged workers and drones. Changes in the relative expression of the lys-1 gene in infested individuals was poorly manifested at the level of enzyme activity, whereas at the two final stages of development (P5 and I) there was a positive correlation between relative lys-1 expression and lysozyme activity in infested bees of both genders (r=0.988, r=0.999, respectively). The results of this study indicate that V. destructor influences the lysozyme-linked immune response in bees.https://doi.org/10.1515/jas-2017-0014developmentdronesgender-related differencesimmune systemworkers |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zaobidna Ewa A. Żółtowska Krystyna Łopieńska-Biernat Elżbieta |
spellingShingle |
Zaobidna Ewa A. Żółtowska Krystyna Łopieńska-Biernat Elżbieta Expression and Activity of Lysozyme in Apis Mellifera Carnica Brood Infested with Varroa Destructor Journal of Apicultural Science development drones gender-related differences immune system workers |
author_facet |
Zaobidna Ewa A. Żółtowska Krystyna Łopieńska-Biernat Elżbieta |
author_sort |
Zaobidna Ewa A. |
title |
Expression and Activity of Lysozyme in Apis Mellifera Carnica Brood Infested with Varroa Destructor |
title_short |
Expression and Activity of Lysozyme in Apis Mellifera Carnica Brood Infested with Varroa Destructor |
title_full |
Expression and Activity of Lysozyme in Apis Mellifera Carnica Brood Infested with Varroa Destructor |
title_fullStr |
Expression and Activity of Lysozyme in Apis Mellifera Carnica Brood Infested with Varroa Destructor |
title_full_unstemmed |
Expression and Activity of Lysozyme in Apis Mellifera Carnica Brood Infested with Varroa Destructor |
title_sort |
expression and activity of lysozyme in apis mellifera carnica brood infested with varroa destructor |
publisher |
Sciendo |
series |
Journal of Apicultural Science |
issn |
2299-4831 |
publishDate |
2017-12-01 |
description |
Varroa destructor is a parasitic mite that attacks the honey bee, and previous studies have suggested that parasitosis caused by this mite is accompanied by immunosuppresion in the host. In this study, the effect of mite infestation on the expression of the lysozyme-1 (lys-1) gene and lysozyme activity in Apis mellifera carnica was determined. The experiment was carried out on the five developmental stages of honey bee workers and drones. Developmental and gender-related differences in gene expression and lysozyme activity were observed in a Varroa destructor-infested brood. The relative expression of the lys-1 gene increased in a infested worker brood and decreased in a drone brood except for P3 pupae. In the final stage of development, the lys-1 gene expression was significantly lower in infested newly emerged workers and drones. Changes in the relative expression of the lys-1 gene in infested individuals was poorly manifested at the level of enzyme activity, whereas at the two final stages of development (P5 and I) there was a positive correlation between relative lys-1 expression and lysozyme activity in infested bees of both genders (r=0.988, r=0.999, respectively). The results of this study indicate that V. destructor influences the lysozyme-linked immune response in bees. |
topic |
development drones gender-related differences immune system workers |
url |
https://doi.org/10.1515/jas-2017-0014 |
work_keys_str_mv |
AT zaobidnaewaa expressionandactivityoflysozymeinapismelliferacarnicabroodinfestedwithvarroadestructor AT zołtowskakrystyna expressionandactivityoflysozymeinapismelliferacarnicabroodinfestedwithvarroadestructor AT łopienskabiernatelzbieta expressionandactivityoflysozymeinapismelliferacarnicabroodinfestedwithvarroadestructor |
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