CRISPR activation screen in mice identifies novel membrane proteins enhancing pulmonary metastatic colonisation

Louise van der Weyden et al. report a CRISPRa screen in mice to identify membrane protein-encoding genes that, when overexpressed, enhance the metastatic potential of melanoma cells. They find that high expression of LRRN4CL in melanoma cells increases lung metastases in mice and correlates with poo...

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Main Authors:	Louise van der Weyden, Victoria Harle, Gemma Turner, Victoria Offord, Vivek Iyer, Alastair Droop, Agnieszka Swiatkowska, Roy Rabbie, Andrew D. Campbell, Owen J. Sansom, Mercedes Pardo, Jyoti S. Choudhary, Ingrid Ferreira, Mark Tullett, Mark J. Arends, Anneliese O. Speak, David J. Adams
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-03-01
Series:	Communications Biology
Online Access:	https://doi.org/10.1038/s42003-021-01912-w

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spelling	doaj-4f4c4eec5cbe46db9a452ba009fd123d2021-03-28T11:24:15ZengNature Publishing GroupCommunications Biology2399-36422021-03-014111210.1038/s42003-021-01912-wCRISPR activation screen in mice identifies novel membrane proteins enhancing pulmonary metastatic colonisationLouise van der Weyden0Victoria Harle1Gemma Turner2Victoria Offord3Vivek Iyer4Alastair Droop5Agnieszka Swiatkowska6Roy Rabbie7Andrew D. Campbell8Owen J. Sansom9Mercedes Pardo10Jyoti S. Choudhary11Ingrid Ferreira12Mark Tullett13Mark J. Arends14Anneliese O. Speak15David J. Adams16Wellcome Sanger Institute, Wellcome Genome Campus, HinxtonWellcome Sanger Institute, Wellcome Genome Campus, HinxtonWellcome Sanger Institute, Wellcome Genome Campus, HinxtonWellcome Sanger Institute, Wellcome Genome Campus, HinxtonWellcome Sanger Institute, Wellcome Genome Campus, HinxtonWellcome Sanger Institute, Wellcome Genome Campus, HinxtonWellcome Sanger Institute, Wellcome Genome Campus, HinxtonWellcome Sanger Institute, Wellcome Genome Campus, HinxtonCancer Research UK Beatson InstituteCancer Research UK Beatson InstituteInstitute of Cancer ResearchInstitute of Cancer ResearchWellcome Sanger Institute, Wellcome Genome Campus, HinxtonWestern Sussex NHS Foundation TrustUniversity of Edinburgh Division of Pathology, Edinburgh Cancer Research UK Cancer Centre, Institute of Genetics & Molecular MedicineWellcome Sanger Institute, Wellcome Genome Campus, HinxtonWellcome Sanger Institute, Wellcome Genome Campus, HinxtonLouise van der Weyden et al. report a CRISPRa screen in mice to identify membrane protein-encoding genes that, when overexpressed, enhance the metastatic potential of melanoma cells. They find that high expression of LRRN4CL in melanoma cells increases lung metastases in mice and correlates with poorer survival in melanoma patients, suggesting it could be a promising drug target.https://doi.org/10.1038/s42003-021-01912-w
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Louise van der Weyden Victoria Harle Gemma Turner Victoria Offord Vivek Iyer Alastair Droop Agnieszka Swiatkowska Roy Rabbie Andrew D. Campbell Owen J. Sansom Mercedes Pardo Jyoti S. Choudhary Ingrid Ferreira Mark Tullett Mark J. Arends Anneliese O. Speak David J. Adams
spellingShingle	Louise van der Weyden Victoria Harle Gemma Turner Victoria Offord Vivek Iyer Alastair Droop Agnieszka Swiatkowska Roy Rabbie Andrew D. Campbell Owen J. Sansom Mercedes Pardo Jyoti S. Choudhary Ingrid Ferreira Mark Tullett Mark J. Arends Anneliese O. Speak David J. Adams CRISPR activation screen in mice identifies novel membrane proteins enhancing pulmonary metastatic colonisation Communications Biology
author_facet	Louise van der Weyden Victoria Harle Gemma Turner Victoria Offord Vivek Iyer Alastair Droop Agnieszka Swiatkowska Roy Rabbie Andrew D. Campbell Owen J. Sansom Mercedes Pardo Jyoti S. Choudhary Ingrid Ferreira Mark Tullett Mark J. Arends Anneliese O. Speak David J. Adams
author_sort	Louise van der Weyden
title	CRISPR activation screen in mice identifies novel membrane proteins enhancing pulmonary metastatic colonisation
title_short	CRISPR activation screen in mice identifies novel membrane proteins enhancing pulmonary metastatic colonisation
title_full	CRISPR activation screen in mice identifies novel membrane proteins enhancing pulmonary metastatic colonisation
title_fullStr	CRISPR activation screen in mice identifies novel membrane proteins enhancing pulmonary metastatic colonisation
title_full_unstemmed	CRISPR activation screen in mice identifies novel membrane proteins enhancing pulmonary metastatic colonisation
title_sort	crispr activation screen in mice identifies novel membrane proteins enhancing pulmonary metastatic colonisation
publisher	Nature Publishing Group
series	Communications Biology
issn	2399-3642
publishDate	2021-03-01
description	Louise van der Weyden et al. report a CRISPRa screen in mice to identify membrane protein-encoding genes that, when overexpressed, enhance the metastatic potential of melanoma cells. They find that high expression of LRRN4CL in melanoma cells increases lung metastases in mice and correlates with poorer survival in melanoma patients, suggesting it could be a promising drug target.
url	https://doi.org/10.1038/s42003-021-01912-w
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CRISPR activation screen in mice identifies novel membrane proteins enhancing pulmonary metastatic colonisation

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