Neurotransmitter-Regulated Regeneration in the Zebrafish Retina
Summary: Current efforts to repair damaged or diseased mammalian retinas are inefficient and largely incapable of fully restoring vision. Conversely, the zebrafish retina is capable of spontaneous regeneration upon damage using Müller glia (MG)-derived progenitors. Understanding how zebrafish MG ini...
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2017-04-01
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Series: | Stem Cell Reports |
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doaj-4f55f6dd72f945528162463bfaabc6b72020-11-24T22:10:47ZengElsevierStem Cell Reports2213-67112017-04-0184831842Neurotransmitter-Regulated Regeneration in the Zebrafish RetinaMahesh B. Rao0Dominic Didiano1James G. Patton2Department of Biological Sciences, Vanderbilt University, 2325 Stevenson Center, Box 1820 Station B, Nashville, TN 37235, USADepartment of Biological Sciences, Vanderbilt University, 2325 Stevenson Center, Box 1820 Station B, Nashville, TN 37235, USADepartment of Biological Sciences, Vanderbilt University, 2325 Stevenson Center, Box 1820 Station B, Nashville, TN 37235, USA; Corresponding authorSummary: Current efforts to repair damaged or diseased mammalian retinas are inefficient and largely incapable of fully restoring vision. Conversely, the zebrafish retina is capable of spontaneous regeneration upon damage using Müller glia (MG)-derived progenitors. Understanding how zebrafish MG initiate regeneration may help develop new treatments that prompt mammalian retinas to regenerate. We show that inhibition of γ-aminobutyric acid (GABA) signaling facilitates initiation of MG proliferation. GABA levels decrease following damage, and MG are positioned to detect decreased ambient levels and undergo dedifferentiation. Using pharmacological and genetic approaches, we demonstrate that GABAA receptor inhibition stimulates regeneration in undamaged retinas while activation inhibits regeneration in damaged retinas. : Unlike mammals, zebrafish regenerate following retina damage from a resident adult stem cell (Müller glia). Dissecting the mechanisms that zebrafish use could lead to new therapeutic targets to treat retinal diseases. Patton and colleagues have discovered a mechanism by which decreased GABA levels are sensed by Müller glia to initiate a regenerative response. Keywords: zebrafish, retina, regeneration, Müller glia, GABAhttp://www.sciencedirect.com/science/article/pii/S2213671117300747 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mahesh B. Rao Dominic Didiano James G. Patton |
spellingShingle |
Mahesh B. Rao Dominic Didiano James G. Patton Neurotransmitter-Regulated Regeneration in the Zebrafish Retina Stem Cell Reports |
author_facet |
Mahesh B. Rao Dominic Didiano James G. Patton |
author_sort |
Mahesh B. Rao |
title |
Neurotransmitter-Regulated Regeneration in the Zebrafish Retina |
title_short |
Neurotransmitter-Regulated Regeneration in the Zebrafish Retina |
title_full |
Neurotransmitter-Regulated Regeneration in the Zebrafish Retina |
title_fullStr |
Neurotransmitter-Regulated Regeneration in the Zebrafish Retina |
title_full_unstemmed |
Neurotransmitter-Regulated Regeneration in the Zebrafish Retina |
title_sort |
neurotransmitter-regulated regeneration in the zebrafish retina |
publisher |
Elsevier |
series |
Stem Cell Reports |
issn |
2213-6711 |
publishDate |
2017-04-01 |
description |
Summary: Current efforts to repair damaged or diseased mammalian retinas are inefficient and largely incapable of fully restoring vision. Conversely, the zebrafish retina is capable of spontaneous regeneration upon damage using Müller glia (MG)-derived progenitors. Understanding how zebrafish MG initiate regeneration may help develop new treatments that prompt mammalian retinas to regenerate. We show that inhibition of γ-aminobutyric acid (GABA) signaling facilitates initiation of MG proliferation. GABA levels decrease following damage, and MG are positioned to detect decreased ambient levels and undergo dedifferentiation. Using pharmacological and genetic approaches, we demonstrate that GABAA receptor inhibition stimulates regeneration in undamaged retinas while activation inhibits regeneration in damaged retinas. : Unlike mammals, zebrafish regenerate following retina damage from a resident adult stem cell (Müller glia). Dissecting the mechanisms that zebrafish use could lead to new therapeutic targets to treat retinal diseases. Patton and colleagues have discovered a mechanism by which decreased GABA levels are sensed by Müller glia to initiate a regenerative response. Keywords: zebrafish, retina, regeneration, Müller glia, GABA |
url |
http://www.sciencedirect.com/science/article/pii/S2213671117300747 |
work_keys_str_mv |
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