Ethyl acetate leaf fraction of Cnidoscolus aconitifolius (Mill.) I. M. Johnst: antioxidant potential, inhibitory activities of key enzymes on carbohydrate metabolism, cholinergic, monoaminergic, purinergic, and chemical fingerprinting
In this study, the antioxidant, carbohydrate metabolism, cholinergic, monoaminergic, and purinergic enzyme activities of ethyl acetate leaf fraction of Cnidoscolus aconitifolius were evaluated with the high-performance liquid chromatography (HPLC) analysis. The ethyl acetate fraction of the plant le...
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doaj-4f7d2d21ae9e4f4c9a91d1970275b7f72020-11-25T02:59:20ZengTaylor & Francis GroupInternational Journal of Food Properties1094-29121532-23862018-01-012111697171510.1080/10942912.2018.15047871504787Ethyl acetate leaf fraction of Cnidoscolus aconitifolius (Mill.) I. M. Johnst: antioxidant potential, inhibitory activities of key enzymes on carbohydrate metabolism, cholinergic, monoaminergic, purinergic, and chemical fingerprintingBasiru Olaitan Ajiboye0Oluwafemi Adeleke Ojo1Mary Abiola Okesola2Babatunji Emmanuel Oyinloye3Abidemi Paul Kappo4Afe Babalola UniversityAfe Babalola UniversityAfe Babalola UniversityAfe Babalola UniversityUniversity of ZululandIn this study, the antioxidant, carbohydrate metabolism, cholinergic, monoaminergic, and purinergic enzyme activities of ethyl acetate leaf fraction of Cnidoscolus aconitifolius were evaluated with the high-performance liquid chromatography (HPLC) analysis. The ethyl acetate fraction of the plant leaf was tested for antioxidant properties, key carbohydrate metabolism, cholinergic, monoaminergic, and purinergic enzyme activities using standard procedures, while the chemical composition of the fraction was evaluated using HPLC. The results revealed that the fraction has higher phenolic compounds than flavonoid and exhibited the ability to scavenge iron chelation and ABTS. The fraction also inhibited the activities of α-amylase and α-glucosidase, acetylcholinesterase, butyrylcholinesterase, monoamine oxidase, tyrosinase, arginase, Ecto-5’-nucleotidase, phosphodieterase-5, angiotensin-I-converting enzyme and encouraged the activity of Na+/K+-ATPase. The HPLC analysis revealed that the ethyl acetate fraction contained coumaric acid, amentoflavone, hesperidin, protocatechuic acid, kaempferol, dihydromyricetin, quercetin,and rutin. The obtained results in this study suggest that the ethyl acetate fraction obtained from aqueous extracts of Cnidoscolus aconitifolius leaf possessed outstanding antioxidative potentials. Thus, excellent enzyme inhibitory activities probably due to bioactive compounds are also observed in the leaf.http://dx.doi.org/10.1080/10942912.2018.1504787Cnidoscolus aconitifoliuscholinergicmonoaminergicpurinergicenzymeshigh performance liquid chromatography |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Basiru Olaitan Ajiboye Oluwafemi Adeleke Ojo Mary Abiola Okesola Babatunji Emmanuel Oyinloye Abidemi Paul Kappo |
spellingShingle |
Basiru Olaitan Ajiboye Oluwafemi Adeleke Ojo Mary Abiola Okesola Babatunji Emmanuel Oyinloye Abidemi Paul Kappo Ethyl acetate leaf fraction of Cnidoscolus aconitifolius (Mill.) I. M. Johnst: antioxidant potential, inhibitory activities of key enzymes on carbohydrate metabolism, cholinergic, monoaminergic, purinergic, and chemical fingerprinting International Journal of Food Properties Cnidoscolus aconitifolius cholinergic monoaminergic purinergic enzymes high performance liquid chromatography |
author_facet |
Basiru Olaitan Ajiboye Oluwafemi Adeleke Ojo Mary Abiola Okesola Babatunji Emmanuel Oyinloye Abidemi Paul Kappo |
author_sort |
Basiru Olaitan Ajiboye |
title |
Ethyl acetate leaf fraction of Cnidoscolus aconitifolius (Mill.) I. M. Johnst: antioxidant potential, inhibitory activities of key enzymes on carbohydrate metabolism, cholinergic, monoaminergic, purinergic, and chemical fingerprinting |
title_short |
Ethyl acetate leaf fraction of Cnidoscolus aconitifolius (Mill.) I. M. Johnst: antioxidant potential, inhibitory activities of key enzymes on carbohydrate metabolism, cholinergic, monoaminergic, purinergic, and chemical fingerprinting |
title_full |
Ethyl acetate leaf fraction of Cnidoscolus aconitifolius (Mill.) I. M. Johnst: antioxidant potential, inhibitory activities of key enzymes on carbohydrate metabolism, cholinergic, monoaminergic, purinergic, and chemical fingerprinting |
title_fullStr |
Ethyl acetate leaf fraction of Cnidoscolus aconitifolius (Mill.) I. M. Johnst: antioxidant potential, inhibitory activities of key enzymes on carbohydrate metabolism, cholinergic, monoaminergic, purinergic, and chemical fingerprinting |
title_full_unstemmed |
Ethyl acetate leaf fraction of Cnidoscolus aconitifolius (Mill.) I. M. Johnst: antioxidant potential, inhibitory activities of key enzymes on carbohydrate metabolism, cholinergic, monoaminergic, purinergic, and chemical fingerprinting |
title_sort |
ethyl acetate leaf fraction of cnidoscolus aconitifolius (mill.) i. m. johnst: antioxidant potential, inhibitory activities of key enzymes on carbohydrate metabolism, cholinergic, monoaminergic, purinergic, and chemical fingerprinting |
publisher |
Taylor & Francis Group |
series |
International Journal of Food Properties |
issn |
1094-2912 1532-2386 |
publishDate |
2018-01-01 |
description |
In this study, the antioxidant, carbohydrate metabolism, cholinergic, monoaminergic, and purinergic enzyme activities of ethyl acetate leaf fraction of Cnidoscolus aconitifolius were evaluated with the high-performance liquid chromatography (HPLC) analysis. The ethyl acetate fraction of the plant leaf was tested for antioxidant properties, key carbohydrate metabolism, cholinergic, monoaminergic, and purinergic enzyme activities using standard procedures, while the chemical composition of the fraction was evaluated using HPLC. The results revealed that the fraction has higher phenolic compounds than flavonoid and exhibited the ability to scavenge iron chelation and ABTS. The fraction also inhibited the activities of α-amylase and α-glucosidase, acetylcholinesterase, butyrylcholinesterase, monoamine oxidase, tyrosinase, arginase, Ecto-5’-nucleotidase, phosphodieterase-5, angiotensin-I-converting enzyme and encouraged the activity of Na+/K+-ATPase. The HPLC analysis revealed that the ethyl acetate fraction contained coumaric acid, amentoflavone, hesperidin, protocatechuic acid, kaempferol, dihydromyricetin, quercetin,and rutin. The obtained results in this study suggest that the ethyl acetate fraction obtained from aqueous extracts of Cnidoscolus aconitifolius leaf possessed outstanding antioxidative potentials. Thus, excellent enzyme inhibitory activities probably due to bioactive compounds are also observed in the leaf. |
topic |
Cnidoscolus aconitifolius cholinergic monoaminergic purinergic enzymes high performance liquid chromatography |
url |
http://dx.doi.org/10.1080/10942912.2018.1504787 |
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