Expression of aryl hydrocarbon receptor, inflammatory cytokines, and incidence of rheumatoid arthritis in Vietnamese dioxin-exposed people
Many Vietnamese citizens have been and continue to be inadvertently exposed to dioxins and dioxin-like compounds deposited in the country during the Vietnam War. Dioxins may be involved in the pathogenesis of inflammatory diseases in part via by affecting expression of aryl hydrocarbon receptor (Ahr...
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doaj-4f7fd7456eef4f8ab17778f8af4c90bc2020-11-25T02:53:07ZengTaylor & Francis GroupJournal of Immunotoxicology1547-691X1547-69012017-01-0114119620310.1080/1547691X.2017.13773231377323Expression of aryl hydrocarbon receptor, inflammatory cytokines, and incidence of rheumatoid arthritis in Vietnamese dioxin-exposed peopleChi Hung Nguyen0Taisuke Nakahama1Truong Tien Dang2Hoang Ha Chu3Luong Van Hoang4Tadamitsu Kishimoto5Nam Trung Nguyen6Institute of Biotechnology, Vietnam Academy of Science and TechnologyOsaka UniversityVietnam Military Medical UniversityInstitute of Biotechnology, Vietnam Academy of Science and TechnologyVietnam Military Medical UniversityOsaka UniversityInstitute of Biotechnology, Vietnam Academy of Science and TechnologyMany Vietnamese citizens have been and continue to be inadvertently exposed to dioxins and dioxin-like compounds deposited in the country during the Vietnam War. Dioxins may be involved in the pathogenesis of inflammatory diseases in part via by affecting expression of aryl hydrocarbon receptor (Ahr) and inflammatory cytokines in animal models. As the role of the Ahr in dioxin-exposed people is not well defined, a study was conducted to examine gene expression levels of Ahr, inflammatory cytokines, and the incidence of diseases in dioxin-exposed citizens who had/still resided near a heavily dioxin-contaminated area in Vietnam. Whole blood from citizens at/around Da Nang airbase and control individuals living in unsprayed areas was collected. Serum levels of dioxins were analyzed by using a dioxins-responsive chemical-activated luciferase gene expression bioassay. Gene expression of Ahr, interleukin (IL)-1β, TNFα, IL-6, and IL-22 in whole blood was examined by quantitative real-time PCR. The results showed levels of dioxins and expression of Ahr, IL-1β, TNFα, and IL-6 were up-regulated while IL-22 expression was down-regulated in dioxin-exposed people. Various disease incidences in the study subjects was also examined. Interestingly, the incidence of rheumatoid arthritis (RA) in these individuals was increased compared to the estimated prevalence of this disease in the general Vietnamese population. Analyses also showed that expression levels of Ahr correlated to those of IL-6 and IL-22 in the dioxin-exposed people. Taken together, dioxins might be involved in an up-regulated expression of Ahr that might possibly relate to changes in level of inflammatory cytokines and, ultimately, in the incidence of select diseases in residents of Vietnam who had/continue to live near a dioxins-contaminated site.http://dx.doi.org/10.1080/1547691X.2017.1377323Da Nang airbasedioxinsdioxin receptorinflammatory cytokinesrheumatoid arthritis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chi Hung Nguyen Taisuke Nakahama Truong Tien Dang Hoang Ha Chu Luong Van Hoang Tadamitsu Kishimoto Nam Trung Nguyen |
spellingShingle |
Chi Hung Nguyen Taisuke Nakahama Truong Tien Dang Hoang Ha Chu Luong Van Hoang Tadamitsu Kishimoto Nam Trung Nguyen Expression of aryl hydrocarbon receptor, inflammatory cytokines, and incidence of rheumatoid arthritis in Vietnamese dioxin-exposed people Journal of Immunotoxicology Da Nang airbase dioxins dioxin receptor inflammatory cytokines rheumatoid arthritis |
author_facet |
Chi Hung Nguyen Taisuke Nakahama Truong Tien Dang Hoang Ha Chu Luong Van Hoang Tadamitsu Kishimoto Nam Trung Nguyen |
author_sort |
Chi Hung Nguyen |
title |
Expression of aryl hydrocarbon receptor, inflammatory cytokines, and incidence of rheumatoid arthritis in Vietnamese dioxin-exposed people |
title_short |
Expression of aryl hydrocarbon receptor, inflammatory cytokines, and incidence of rheumatoid arthritis in Vietnamese dioxin-exposed people |
title_full |
Expression of aryl hydrocarbon receptor, inflammatory cytokines, and incidence of rheumatoid arthritis in Vietnamese dioxin-exposed people |
title_fullStr |
Expression of aryl hydrocarbon receptor, inflammatory cytokines, and incidence of rheumatoid arthritis in Vietnamese dioxin-exposed people |
title_full_unstemmed |
Expression of aryl hydrocarbon receptor, inflammatory cytokines, and incidence of rheumatoid arthritis in Vietnamese dioxin-exposed people |
title_sort |
expression of aryl hydrocarbon receptor, inflammatory cytokines, and incidence of rheumatoid arthritis in vietnamese dioxin-exposed people |
publisher |
Taylor & Francis Group |
series |
Journal of Immunotoxicology |
issn |
1547-691X 1547-6901 |
publishDate |
2017-01-01 |
description |
Many Vietnamese citizens have been and continue to be inadvertently exposed to dioxins and dioxin-like compounds deposited in the country during the Vietnam War. Dioxins may be involved in the pathogenesis of inflammatory diseases in part via by affecting expression of aryl hydrocarbon receptor (Ahr) and inflammatory cytokines in animal models. As the role of the Ahr in dioxin-exposed people is not well defined, a study was conducted to examine gene expression levels of Ahr, inflammatory cytokines, and the incidence of diseases in dioxin-exposed citizens who had/still resided near a heavily dioxin-contaminated area in Vietnam. Whole blood from citizens at/around Da Nang airbase and control individuals living in unsprayed areas was collected. Serum levels of dioxins were analyzed by using a dioxins-responsive chemical-activated luciferase gene expression bioassay. Gene expression of Ahr, interleukin (IL)-1β, TNFα, IL-6, and IL-22 in whole blood was examined by quantitative real-time PCR. The results showed levels of dioxins and expression of Ahr, IL-1β, TNFα, and IL-6 were up-regulated while IL-22 expression was down-regulated in dioxin-exposed people. Various disease incidences in the study subjects was also examined. Interestingly, the incidence of rheumatoid arthritis (RA) in these individuals was increased compared to the estimated prevalence of this disease in the general Vietnamese population. Analyses also showed that expression levels of Ahr correlated to those of IL-6 and IL-22 in the dioxin-exposed people. Taken together, dioxins might be involved in an up-regulated expression of Ahr that might possibly relate to changes in level of inflammatory cytokines and, ultimately, in the incidence of select diseases in residents of Vietnam who had/continue to live near a dioxins-contaminated site. |
topic |
Da Nang airbase dioxins dioxin receptor inflammatory cytokines rheumatoid arthritis |
url |
http://dx.doi.org/10.1080/1547691X.2017.1377323 |
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