Advances on plant redox proteomics technologies for cysteine reversible oxidation
Reactive oxygen species (ROS)-mediated reversible oxidation of protein cysteine (Cys) plays an important role in the regulation of plant development and stress response. In recent years, high-throughput redox proteomics technologies have been developed, including several labeling strategies based on...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Academic Journals Center of Shanghai Normal University
2018-10-01
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Series: | Journal of Shanghai Normal University (Natural Sciences) |
Subjects: | |
Online Access: | http://qktg.shnu.edu.cn/zrb/shsfqkszrb/ch/reader/view_abstract.aspx?file_no=20180508 |
Summary: | Reactive oxygen species (ROS)-mediated reversible oxidation of protein cysteine (Cys) plays an important role in the regulation of plant development and stress response. In recent years, high-throughput redox proteomics technologies have been developed, including several labeling strategies based on fluorescent reagents, biotin-conjugated alkylating reagents, isotope-coded affinity tags (ICAT), thiol-specific tandem mass tags (CysTMT/iodoTMT), as well as iodoTMT combined with isobaric tag for relative and absolute quantification (iTRAQ) reagents, respectively. The development of these technologies provides effective tools for systematically understanding the mechanisms of protein redox homeostasis in plants. In this review, the redox proteomics technologies applied in the research of plant Cys reversible oxidation have been summarized. |
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ISSN: | 1000-5137 1000-5137 |