Inhibition of poly-LacNAc biosynthesis with release of CMP-Neu5Ac feedback inhibition increases the sialylation of recombinant EPO produced in CHO cells
Abstract Sialylation of recombinant therapeutic glycoproteins modulates their pharmacokinetic properties by affecting their in vivo half-life. N-glycan branching on glycoproteins increases the number of potential attachment sites for sialic acid. Here, we introduce a new approach for increasing the...
Main Authors: | Chung-Geun Lee, Myung Jin Oh, Seung-Yeol Park, Hyun Joo An, Jung Hoe Kim |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2018-05-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-018-25580-9 |
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