Mass spectrometry‐based protein–protein interaction networks for the study of human diseases
Abstract A better understanding of the molecular mechanisms underlying disease is key for expediting the development of novel therapeutic interventions. Disease mechanisms are often mediated by interactions between proteins. Insights into the physical rewiring of protein–protein interactions in resp...
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Online Access: | https://doi.org/10.15252/msb.20188792 |
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doaj-4f98bc411b5b443090e3549020a0570c2021-08-02T23:39:51ZengWileyMolecular Systems Biology1744-42922021-01-01171n/an/a10.15252/msb.20188792Mass spectrometry‐based protein–protein interaction networks for the study of human diseasesAlicia L Richards0Manon Eckhardt1Nevan J Krogan2Quantitative Biosciences Institute (QBI) University of California San Francisco San Francisco CA USAQuantitative Biosciences Institute (QBI) University of California San Francisco San Francisco CA USAQuantitative Biosciences Institute (QBI) University of California San Francisco San Francisco CA USAAbstract A better understanding of the molecular mechanisms underlying disease is key for expediting the development of novel therapeutic interventions. Disease mechanisms are often mediated by interactions between proteins. Insights into the physical rewiring of protein–protein interactions in response to mutations, pathological conditions, or pathogen infection can advance our understanding of disease etiology, progression, and pathogenesis and can lead to the identification of potential druggable targets. Advances in quantitative mass spectrometry (MS)‐based approaches have allowed unbiased mapping of these disease‐mediated changes in protein–protein interactions on a global scale. Here, we review MS techniques that have been instrumental for the identification of protein–protein interactions at a system‐level, and we discuss the challenges associated with these methodologies as well as novel MS advancements that aim to address these challenges. An overview of examples from diverse disease contexts illustrates the potential of MS‐based protein–protein interaction mapping approaches for revealing disease mechanisms, pinpointing new therapeutic targets, and eventually moving toward personalized applications.https://doi.org/10.15252/msb.20188792affinity purificationmass spectrometrynetworksprotein–protein interactionsproximity labeling |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alicia L Richards Manon Eckhardt Nevan J Krogan |
spellingShingle |
Alicia L Richards Manon Eckhardt Nevan J Krogan Mass spectrometry‐based protein–protein interaction networks for the study of human diseases Molecular Systems Biology affinity purification mass spectrometry networks protein–protein interactions proximity labeling |
author_facet |
Alicia L Richards Manon Eckhardt Nevan J Krogan |
author_sort |
Alicia L Richards |
title |
Mass spectrometry‐based protein–protein interaction networks for the study of human diseases |
title_short |
Mass spectrometry‐based protein–protein interaction networks for the study of human diseases |
title_full |
Mass spectrometry‐based protein–protein interaction networks for the study of human diseases |
title_fullStr |
Mass spectrometry‐based protein–protein interaction networks for the study of human diseases |
title_full_unstemmed |
Mass spectrometry‐based protein–protein interaction networks for the study of human diseases |
title_sort |
mass spectrometry‐based protein–protein interaction networks for the study of human diseases |
publisher |
Wiley |
series |
Molecular Systems Biology |
issn |
1744-4292 |
publishDate |
2021-01-01 |
description |
Abstract A better understanding of the molecular mechanisms underlying disease is key for expediting the development of novel therapeutic interventions. Disease mechanisms are often mediated by interactions between proteins. Insights into the physical rewiring of protein–protein interactions in response to mutations, pathological conditions, or pathogen infection can advance our understanding of disease etiology, progression, and pathogenesis and can lead to the identification of potential druggable targets. Advances in quantitative mass spectrometry (MS)‐based approaches have allowed unbiased mapping of these disease‐mediated changes in protein–protein interactions on a global scale. Here, we review MS techniques that have been instrumental for the identification of protein–protein interactions at a system‐level, and we discuss the challenges associated with these methodologies as well as novel MS advancements that aim to address these challenges. An overview of examples from diverse disease contexts illustrates the potential of MS‐based protein–protein interaction mapping approaches for revealing disease mechanisms, pinpointing new therapeutic targets, and eventually moving toward personalized applications. |
topic |
affinity purification mass spectrometry networks protein–protein interactions proximity labeling |
url |
https://doi.org/10.15252/msb.20188792 |
work_keys_str_mv |
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