Ethanol extract separated from Sargassum horneri (Turner) abate LPS-induced inflammation in RAW 264.7 macrophages

• Main Authors: K. K. Asanka Sanjeewa, Thilina U. Jayawardena, Hyun-Soo Kim, Seo-Young Kim, Ginnae Ahn, Hak-Ju Kim, Xiaoting Fu, Youngheun Jee, You-Jin Jeon
• Format: Article
• Language: English
• Published: The Korean Society of Fisheries and Aquatic Science 2019-02-01
• Series: Fisheries and Aquatic Sciences
• Subjects: Sargassum horneri; Ethanol extract; RAW 264.7 macrophages; Anti-inflammation; NF-κB; MAPKs
• Online Access: http://link.springer.com/article/10.1186/s41240-019-0121-8

Abstract Background This study is aimed at identifying the anti-inflammatory properties of 70% ethanol extract produced from an edible brown seaweed Sargassum horneri (SJB-SHE) with industrial-scale production by Seojin Biotech Co. Ltd. S. horneri is a rich source of nutrient and abundantly growing along the shores of Jeju, South Korea. Methods Here, we investigated the effect of SJB-SHE on LPS-activated RAW 264.7 macrophages. The cytotoxicity and NO production of SJB-SHE were evaluated using MTT and Griess assays, respectively. Additionally, protein expression and gene expression levels were quantified using ELISA, Western blots, and RT-qPCR. Results Our results indicated that pre-treatment of RAW 264.7 macrophages with SJB-SHE significantly inhibited LPS-induced NO and PGE2 production. SJB-SHE downregulated the proteins and genes expression of LPS-induced iNOS and COX2. Additionally, SJB-SHE downregulated LPS-induced production of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin (IL)-6, and IL-1β). Furthermore, SJB-SHE inhibited nuclear factor kappa-B (NF-κB) activation and translocation to the nucleus. SJB-SHE also suppressed the phosphorylation of mitogen-activated protein kinases (ERK1/2 and JNK). Conclusions Collectively, our results demonstrated that SJB-SHE has a potential anti-inflammatory property to use as a functional food ingredient in the future.