Endothelial Progenitor Cell Fraction Contained in Bone Marrow-Derived Mesenchymal Stem Cell Populations Impairs Osteogenic Differentiation
In bone tissue engineering (TE) endothelial cell-osteoblast cocultures are known to induce synergies of cell differentiation and activity. Bone marrow mononucleated cells (BMCs) are a rich source of mesenchymal stem cells (MSCs) able to develop an osteogenic phenotype. Endothelial progenitor cells (...
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Online Access: | http://dx.doi.org/10.1155/2015/659542 |
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doaj-4fac03afe75f42919b6cfacfad0ec2302020-11-24T23:15:55ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/659542659542Endothelial Progenitor Cell Fraction Contained in Bone Marrow-Derived Mesenchymal Stem Cell Populations Impairs Osteogenic DifferentiationFabian Duttenhoefer0Rafael Lara de Freitas1Markus Loibl2Gido Bittermann3R. Geoff Richards4Mauro Alini5Sophie Verrier6AO Research Institute Davos, Davos Platz, SwitzerlandAO Research Institute Davos, Davos Platz, SwitzerlandAO Research Institute Davos, Davos Platz, SwitzerlandDepartment of Oral and Maxillofacial Surgery, Albert Ludwigs University, Freiburg, GermanyAO Research Institute Davos, Davos Platz, SwitzerlandAO Research Institute Davos, Davos Platz, SwitzerlandAO Research Institute Davos, Davos Platz, SwitzerlandIn bone tissue engineering (TE) endothelial cell-osteoblast cocultures are known to induce synergies of cell differentiation and activity. Bone marrow mononucleated cells (BMCs) are a rich source of mesenchymal stem cells (MSCs) able to develop an osteogenic phenotype. Endothelial progenitor cells (EPCs) are also present within BMC. In this study we investigate the effect of EPCs present in the BMC population on MSCs osteogenic differentiation. Human BMCs were isolated and separated into two populations. The MSC population was selected through plastic adhesion capacity. EPCs (CD34+ and CD133+) were removed from the BMC population and the resulting population was named depleted MSCs. Both populations were cultured over 28 days in osteogenic medium (Dex+) or medium containing platelet lysate (PL). MSC population grew faster than depleted MSCs in both media, and PL containing medium accelerated the proliferation for both populations. Cell differentiation was much higher in Dex+ medium in both cases. Real-time RT-PCR revealed upregulation of osteogenic marker genes in depleted MSCs. Higher values of ALP activity and matrix mineralization analyses confirmed these results. Our study advocates that absence of EPCs in the MSC population enables higher osteogenic gene expression and matrix mineralization and therefore may lead to advanced bone neoformation necessary for TE constructs.http://dx.doi.org/10.1155/2015/659542 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fabian Duttenhoefer Rafael Lara de Freitas Markus Loibl Gido Bittermann R. Geoff Richards Mauro Alini Sophie Verrier |
spellingShingle |
Fabian Duttenhoefer Rafael Lara de Freitas Markus Loibl Gido Bittermann R. Geoff Richards Mauro Alini Sophie Verrier Endothelial Progenitor Cell Fraction Contained in Bone Marrow-Derived Mesenchymal Stem Cell Populations Impairs Osteogenic Differentiation BioMed Research International |
author_facet |
Fabian Duttenhoefer Rafael Lara de Freitas Markus Loibl Gido Bittermann R. Geoff Richards Mauro Alini Sophie Verrier |
author_sort |
Fabian Duttenhoefer |
title |
Endothelial Progenitor Cell Fraction Contained in Bone Marrow-Derived Mesenchymal Stem Cell Populations Impairs Osteogenic Differentiation |
title_short |
Endothelial Progenitor Cell Fraction Contained in Bone Marrow-Derived Mesenchymal Stem Cell Populations Impairs Osteogenic Differentiation |
title_full |
Endothelial Progenitor Cell Fraction Contained in Bone Marrow-Derived Mesenchymal Stem Cell Populations Impairs Osteogenic Differentiation |
title_fullStr |
Endothelial Progenitor Cell Fraction Contained in Bone Marrow-Derived Mesenchymal Stem Cell Populations Impairs Osteogenic Differentiation |
title_full_unstemmed |
Endothelial Progenitor Cell Fraction Contained in Bone Marrow-Derived Mesenchymal Stem Cell Populations Impairs Osteogenic Differentiation |
title_sort |
endothelial progenitor cell fraction contained in bone marrow-derived mesenchymal stem cell populations impairs osteogenic differentiation |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
In bone tissue engineering (TE) endothelial cell-osteoblast cocultures are known to induce synergies of cell differentiation and activity. Bone marrow mononucleated cells (BMCs) are a rich source of mesenchymal stem cells (MSCs) able to develop an osteogenic phenotype. Endothelial progenitor cells (EPCs) are also present within BMC. In this study we investigate the effect of EPCs present in the BMC population on MSCs osteogenic differentiation. Human BMCs were isolated and separated into two populations. The MSC population was selected through plastic adhesion capacity. EPCs (CD34+ and CD133+) were removed from the BMC population and the resulting population was named depleted MSCs. Both populations were cultured over 28 days in osteogenic medium (Dex+) or medium containing platelet lysate (PL). MSC population grew faster than depleted MSCs in both media, and PL containing medium accelerated the proliferation for both populations. Cell differentiation was much higher in Dex+ medium in both cases. Real-time RT-PCR revealed upregulation of osteogenic marker genes in depleted MSCs. Higher values of ALP activity and matrix mineralization analyses confirmed these results. Our study advocates that absence of EPCs in the MSC population enables higher osteogenic gene expression and matrix mineralization and therefore may lead to advanced bone neoformation necessary for TE constructs. |
url |
http://dx.doi.org/10.1155/2015/659542 |
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