UV-induced mutagenesis in Volvariella volvacea to improve mushroom yield
Low biological efficiency is a defect in straw mushroom production. Mycelia of the straw mushroom were exposed to UV radiation to induce mutagenesis and improve the strains of straw mushroom for a high yield. A UV exposure time that yielded a survival rate of about 25% was applied for mutation ind...
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Prince of Songkla University
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doaj-4fb8cb5a24604082bbe99771dda5553c2020-11-25T03:36:43ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952020-08-0142491091610.14456/sjst-psu.2020.116UV-induced mutagenesis in Volvariella volvacea to improve mushroom yieldEakaphun Bangyeekhun0Korapan Sawetsuwannakul1Urarux Romruen2Department of Microbiology, Faculty of Science, Silpakorn University, Mueang, Nakhon Pathom, 73000 ThailandDepartment of Microbiology, Faculty of Science, Silpakorn University, Mueang, Nakhon Pathom, 73000 ThailandDepartment of Microbiology, Faculty of Science, Silpakorn University, Mueang, Nakhon Pathom, 73000 ThailandLow biological efficiency is a defect in straw mushroom production. Mycelia of the straw mushroom were exposed to UV radiation to induce mutagenesis and improve the strains of straw mushroom for a high yield. A UV exposure time that yielded a survival rate of about 25% was applied for mutation induction. After UV treatment, 65 putative mutant strains were obtained and seven fast-growing strains were selected for further analysis. Mushroom cultivation experiments revealed that the biological efficiency of four of the new strains was approximately 30% higher than the parental strain. The radial mycelial colony growth rate was assessed on different media and under various temperature and pH conditions for physical characterization. The results indicated that the overall growth rates of the mutant strains were higher than the parental strain. Genetic characterization by random amplification of polymorphic DNA analysis revealed that the genome had been altered in the mutant strains. https://rdo.psu.ac.th/sjstweb/journal/42-4/25.pdfmutagenesisstraw mushroomuvvolvariella volvaceayield |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eakaphun Bangyeekhun Korapan Sawetsuwannakul Urarux Romruen |
spellingShingle |
Eakaphun Bangyeekhun Korapan Sawetsuwannakul Urarux Romruen UV-induced mutagenesis in Volvariella volvacea to improve mushroom yield Songklanakarin Journal of Science and Technology (SJST) mutagenesis straw mushroom uv volvariella volvacea yield |
author_facet |
Eakaphun Bangyeekhun Korapan Sawetsuwannakul Urarux Romruen |
author_sort |
Eakaphun Bangyeekhun |
title |
UV-induced mutagenesis in Volvariella volvacea to improve mushroom yield |
title_short |
UV-induced mutagenesis in Volvariella volvacea to improve mushroom yield |
title_full |
UV-induced mutagenesis in Volvariella volvacea to improve mushroom yield |
title_fullStr |
UV-induced mutagenesis in Volvariella volvacea to improve mushroom yield |
title_full_unstemmed |
UV-induced mutagenesis in Volvariella volvacea to improve mushroom yield |
title_sort |
uv-induced mutagenesis in volvariella volvacea to improve mushroom yield |
publisher |
Prince of Songkla University |
series |
Songklanakarin Journal of Science and Technology (SJST) |
issn |
0125-3395 |
publishDate |
2020-08-01 |
description |
Low biological efficiency is a defect in straw mushroom production. Mycelia of the straw mushroom were exposed to
UV radiation to induce mutagenesis and improve the strains of straw mushroom for a high yield. A UV exposure time that
yielded a survival rate of about 25% was applied for mutation induction. After UV treatment, 65 putative mutant strains were
obtained and seven fast-growing strains were selected for further analysis. Mushroom cultivation experiments revealed that the
biological efficiency of four of the new strains was approximately 30% higher than the parental strain. The radial mycelial colony
growth rate was assessed on different media and under various temperature and pH conditions for physical characterization. The
results indicated that the overall growth rates of the mutant strains were higher than the parental strain. Genetic characterization
by random amplification of polymorphic DNA analysis revealed that the genome had been altered in the mutant strains. |
topic |
mutagenesis straw mushroom uv volvariella volvacea yield |
url |
https://rdo.psu.ac.th/sjstweb/journal/42-4/25.pdf |
work_keys_str_mv |
AT eakaphunbangyeekhun uvinducedmutagenesisinvolvariellavolvaceatoimprovemushroomyield AT korapansawetsuwannakul uvinducedmutagenesisinvolvariellavolvaceatoimprovemushroomyield AT uraruxromruen uvinducedmutagenesisinvolvariellavolvaceatoimprovemushroomyield |
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