Fungal Endophytes Exert Positive Effects on Colobanthus quitensis Under Water Stress but Neutral Under a Projected Climate Change Scenario in Antarctica
Functional symbiosis is considered one of the successful mechanisms by which plants that inhabit extreme environment improve their ability to tolerate different types of stress. One of the most conspicuous type of symbiosis is the endophyticism. This interaction has been noted to play a role in the...
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doaj-4fc0506ead4440849bdb44d2ae8d6b0f2020-11-25T01:40:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-02-011110.3389/fmicb.2020.00264483745Fungal Endophytes Exert Positive Effects on Colobanthus quitensis Under Water Stress but Neutral Under a Projected Climate Change Scenario in AntarcticaRasme Hereme0Samuel Morales-Navarro1Gabriel Ballesteros2Andrea Barrera3Patricio Ramos4Patricio Ramos5Pedro E. Gundel6Marco A. Molina-Montenegro7Marco A. Molina-Montenegro8Marco A. Molina-Montenegro9Instituto de Ciencias Biológicas, Universidad de Talca, Talca, ChileBachillerato en Ciencias, Facultad de Ciencias, Universidad Santo Tomás, Talca, ChileInstituto de Ciencias Biológicas, Universidad de Talca, Talca, ChileInstituto de Ciencias Biológicas, Universidad de Talca, Talca, ChileInstituto de Ciencias Biológicas, Universidad de Talca, Talca, ChileNúcleo Científico Multidisciplinario-DI, Universidad de Talca, Talca, ChileIFEVA, CONICET, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, ArgentinaInstituto de Ciencias Biológicas, Universidad de Talca, Talca, ChileCentro de Estudios Avanzados en Zonas Áridas, Universidad Católica del Norte, Antofagasta, ChileCentro de Investigación en Estudios Avanzados del Maule, Universidad Católica del Maule, Talca, ChileFunctional symbiosis is considered one of the successful mechanisms by which plants that inhabit extreme environment improve their ability to tolerate different types of stress. One of the most conspicuous type of symbiosis is the endophyticism. This interaction has been noted to play a role in the adaptation of the native vascular plant Colobanthus quitensis to the stressful environments of Antarctica, characterized by low temperatures and extreme aridity. Projections of climate change for this ecosystem indicate that abiotic conditions will be less limiting due to an increase in temperature and water availability in the soil. Due to this decrease in stress induced by the climate change, it has been suggested that the positive role of fungal endophytes on performance of C. quitensis plants would decrease. In this study, we evaluated the role of endophytic fungi on osmoprotective molecules (sugar production, proline, oxidative stress) and gene expression (CqNCED1, CqABCG25, and CqRD22) as well as physiological traits (stomatal opening, net photosynthesis, and stomatal conductance) in individuals of C. quitensis. Individual plants of C. quitensis with (E+) and without (E−) endophytic fungi were exposed to simulated conditions of increased water availability (W+), having the current limiting water condition (W−) in Antarctica as control. The results reveal an endophyte-mediated lower oxidative stress, higher production of sugars and proline in plants. In addition, E+ plants showed differential expressions in genes related with drought stress response, which was more evident in W− than in W+. These parameters corresponded with increased physiological mechanisms such as higher net photosynthesis, stomatal opening and conductance under presence of endophytes (E+) as well as the projected water condition (W+) for Antarctica. These results suggest that the presence of fungal endophytes plays a positive role in favoring tolerance to drought in C. quitensis. However, this positive role would be diminished if the stress factor is relaxed, suggesting that the role of endophytes could be less important under a future scenario of climate change in Antarctica with higher soil water availability.https://www.frontiersin.org/article/10.3389/fmicb.2020.00264/fullfunctional symbiosisAntarcticaclimate changeColobanthus quitensisosmoprotective moleculeswater stress |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rasme Hereme Samuel Morales-Navarro Gabriel Ballesteros Andrea Barrera Patricio Ramos Patricio Ramos Pedro E. Gundel Marco A. Molina-Montenegro Marco A. Molina-Montenegro Marco A. Molina-Montenegro |
spellingShingle |
Rasme Hereme Samuel Morales-Navarro Gabriel Ballesteros Andrea Barrera Patricio Ramos Patricio Ramos Pedro E. Gundel Marco A. Molina-Montenegro Marco A. Molina-Montenegro Marco A. Molina-Montenegro Fungal Endophytes Exert Positive Effects on Colobanthus quitensis Under Water Stress but Neutral Under a Projected Climate Change Scenario in Antarctica Frontiers in Microbiology functional symbiosis Antarctica climate change Colobanthus quitensis osmoprotective molecules water stress |
author_facet |
Rasme Hereme Samuel Morales-Navarro Gabriel Ballesteros Andrea Barrera Patricio Ramos Patricio Ramos Pedro E. Gundel Marco A. Molina-Montenegro Marco A. Molina-Montenegro Marco A. Molina-Montenegro |
author_sort |
Rasme Hereme |
title |
Fungal Endophytes Exert Positive Effects on Colobanthus quitensis Under Water Stress but Neutral Under a Projected Climate Change Scenario in Antarctica |
title_short |
Fungal Endophytes Exert Positive Effects on Colobanthus quitensis Under Water Stress but Neutral Under a Projected Climate Change Scenario in Antarctica |
title_full |
Fungal Endophytes Exert Positive Effects on Colobanthus quitensis Under Water Stress but Neutral Under a Projected Climate Change Scenario in Antarctica |
title_fullStr |
Fungal Endophytes Exert Positive Effects on Colobanthus quitensis Under Water Stress but Neutral Under a Projected Climate Change Scenario in Antarctica |
title_full_unstemmed |
Fungal Endophytes Exert Positive Effects on Colobanthus quitensis Under Water Stress but Neutral Under a Projected Climate Change Scenario in Antarctica |
title_sort |
fungal endophytes exert positive effects on colobanthus quitensis under water stress but neutral under a projected climate change scenario in antarctica |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-02-01 |
description |
Functional symbiosis is considered one of the successful mechanisms by which plants that inhabit extreme environment improve their ability to tolerate different types of stress. One of the most conspicuous type of symbiosis is the endophyticism. This interaction has been noted to play a role in the adaptation of the native vascular plant Colobanthus quitensis to the stressful environments of Antarctica, characterized by low temperatures and extreme aridity. Projections of climate change for this ecosystem indicate that abiotic conditions will be less limiting due to an increase in temperature and water availability in the soil. Due to this decrease in stress induced by the climate change, it has been suggested that the positive role of fungal endophytes on performance of C. quitensis plants would decrease. In this study, we evaluated the role of endophytic fungi on osmoprotective molecules (sugar production, proline, oxidative stress) and gene expression (CqNCED1, CqABCG25, and CqRD22) as well as physiological traits (stomatal opening, net photosynthesis, and stomatal conductance) in individuals of C. quitensis. Individual plants of C. quitensis with (E+) and without (E−) endophytic fungi were exposed to simulated conditions of increased water availability (W+), having the current limiting water condition (W−) in Antarctica as control. The results reveal an endophyte-mediated lower oxidative stress, higher production of sugars and proline in plants. In addition, E+ plants showed differential expressions in genes related with drought stress response, which was more evident in W− than in W+. These parameters corresponded with increased physiological mechanisms such as higher net photosynthesis, stomatal opening and conductance under presence of endophytes (E+) as well as the projected water condition (W+) for Antarctica. These results suggest that the presence of fungal endophytes plays a positive role in favoring tolerance to drought in C. quitensis. However, this positive role would be diminished if the stress factor is relaxed, suggesting that the role of endophytes could be less important under a future scenario of climate change in Antarctica with higher soil water availability. |
topic |
functional symbiosis Antarctica climate change Colobanthus quitensis osmoprotective molecules water stress |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2020.00264/full |
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