Diagnostic accuracy of microscopic Observation Drug Susceptibility (MODS) assay for pediatric tuberculosis in Hanoi, Vietnam.
Microscopic [corrected] Observation Drug Susceptibility (MODS) has been shown to be an effective and rapid technique for early diagnosis of tuberculosis (TB). Thus far only a limited number of studies evaluating MODS have been performed in children and in extra-pulmonary tuberculosis. This study aim...
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doaj-4fca79b682fa46a8820d62cbae5cc3ce2020-11-24T20:50:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7210010.1371/journal.pone.0072100Diagnostic accuracy of microscopic Observation Drug Susceptibility (MODS) assay for pediatric tuberculosis in Hanoi, Vietnam.Sinh Thi TranJohn Patrick RenschlerHai Thanh LeHang Thi Thu DangTuan Minh DaoAn Nhat PhamLiem Thanh NguyenHung Van NguyenThuy Thi Thu NguyenSy Ngoc LeAnnette FoxMaxine CawsNhu Thi Quynh NguyenPeter HorbyHeiman WertheimMicroscopic [corrected] Observation Drug Susceptibility (MODS) has been shown to be an effective and rapid technique for early diagnosis of tuberculosis (TB). Thus far only a limited number of studies evaluating MODS have been performed in children and in extra-pulmonary tuberculosis. This study aims to assess relative accuracy and time to positive culture of MODS for TB diagnosis in children admitted to a general pediatric hospital in Vietnam.Specimens from children with suspected TB were tested by smear, MODS and Lowenstein-Jensen agar (LJ). 1129 samples from 705 children were analyzed, including sputum (n=59), gastric aspirate (n=775), CSF (n=148), pleural fluid (n=33), BAL (n=41), tracheal fluid (n=45), other (n=28). 113 TB cases were defined based on the "clinical diagnosis" (confirmed and probable groups) as the reference standard, in which 26% (n=30) were diagnosed as extra-pulmonary TB. Analysis by patient shows that the overall sensitivity and specificity of smear, LJ and MODS against "clinical diagnosis" was 8.8% and 100%, 38.9% and 100%, 46% and 99.5% respectively with MODS significantly more sensitive than LJ culture (P=0.02). When analyzed by sample type, the sensitivity of MODS was significantly higher than LJ for gastric aspirates (P=0.004). The time to detection was also significantly shorter for MODS than LJ (7 days versus 32 days, P<0.001).MODS [corrected] is a sensitive and rapid culture technique for detecting TB in children. As MODS culture can be performed at a BSL2 facility and is inexpensive, it can therefore be recommended as a routine test for children with symptoms suggestive of TB in resource-limited settings.http://europepmc.org/articles/PMC3762843?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sinh Thi Tran John Patrick Renschler Hai Thanh Le Hang Thi Thu Dang Tuan Minh Dao An Nhat Pham Liem Thanh Nguyen Hung Van Nguyen Thuy Thi Thu Nguyen Sy Ngoc Le Annette Fox Maxine Caws Nhu Thi Quynh Nguyen Peter Horby Heiman Wertheim |
spellingShingle |
Sinh Thi Tran John Patrick Renschler Hai Thanh Le Hang Thi Thu Dang Tuan Minh Dao An Nhat Pham Liem Thanh Nguyen Hung Van Nguyen Thuy Thi Thu Nguyen Sy Ngoc Le Annette Fox Maxine Caws Nhu Thi Quynh Nguyen Peter Horby Heiman Wertheim Diagnostic accuracy of microscopic Observation Drug Susceptibility (MODS) assay for pediatric tuberculosis in Hanoi, Vietnam. PLoS ONE |
author_facet |
Sinh Thi Tran John Patrick Renschler Hai Thanh Le Hang Thi Thu Dang Tuan Minh Dao An Nhat Pham Liem Thanh Nguyen Hung Van Nguyen Thuy Thi Thu Nguyen Sy Ngoc Le Annette Fox Maxine Caws Nhu Thi Quynh Nguyen Peter Horby Heiman Wertheim |
author_sort |
Sinh Thi Tran |
title |
Diagnostic accuracy of microscopic Observation Drug Susceptibility (MODS) assay for pediatric tuberculosis in Hanoi, Vietnam. |
title_short |
Diagnostic accuracy of microscopic Observation Drug Susceptibility (MODS) assay for pediatric tuberculosis in Hanoi, Vietnam. |
title_full |
Diagnostic accuracy of microscopic Observation Drug Susceptibility (MODS) assay for pediatric tuberculosis in Hanoi, Vietnam. |
title_fullStr |
Diagnostic accuracy of microscopic Observation Drug Susceptibility (MODS) assay for pediatric tuberculosis in Hanoi, Vietnam. |
title_full_unstemmed |
Diagnostic accuracy of microscopic Observation Drug Susceptibility (MODS) assay for pediatric tuberculosis in Hanoi, Vietnam. |
title_sort |
diagnostic accuracy of microscopic observation drug susceptibility (mods) assay for pediatric tuberculosis in hanoi, vietnam. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
Microscopic [corrected] Observation Drug Susceptibility (MODS) has been shown to be an effective and rapid technique for early diagnosis of tuberculosis (TB). Thus far only a limited number of studies evaluating MODS have been performed in children and in extra-pulmonary tuberculosis. This study aims to assess relative accuracy and time to positive culture of MODS for TB diagnosis in children admitted to a general pediatric hospital in Vietnam.Specimens from children with suspected TB were tested by smear, MODS and Lowenstein-Jensen agar (LJ). 1129 samples from 705 children were analyzed, including sputum (n=59), gastric aspirate (n=775), CSF (n=148), pleural fluid (n=33), BAL (n=41), tracheal fluid (n=45), other (n=28). 113 TB cases were defined based on the "clinical diagnosis" (confirmed and probable groups) as the reference standard, in which 26% (n=30) were diagnosed as extra-pulmonary TB. Analysis by patient shows that the overall sensitivity and specificity of smear, LJ and MODS against "clinical diagnosis" was 8.8% and 100%, 38.9% and 100%, 46% and 99.5% respectively with MODS significantly more sensitive than LJ culture (P=0.02). When analyzed by sample type, the sensitivity of MODS was significantly higher than LJ for gastric aspirates (P=0.004). The time to detection was also significantly shorter for MODS than LJ (7 days versus 32 days, P<0.001).MODS [corrected] is a sensitive and rapid culture technique for detecting TB in children. As MODS culture can be performed at a BSL2 facility and is inexpensive, it can therefore be recommended as a routine test for children with symptoms suggestive of TB in resource-limited settings. |
url |
http://europepmc.org/articles/PMC3762843?pdf=render |
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