Co-delivery of gambogic acid and TRAIL plasmid by hyaluronic acid grafted PEI-PLGA nanoparticles for the treatment of triple negative breast cancer
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-based combination therapy and gene therapy are new strategies to potentially overcome the limitations of TRAIL, however, the lack of efficient and low toxic vectors remains the major obstacle. In this study, we developed a hyaluronic ac...
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doaj-4fcc673f41fc4d92a1c76a4baaa1f2c92020-11-25T02:06:36ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642017-01-012411791180010.1080/10717544.2017.14065581406558Co-delivery of gambogic acid and TRAIL plasmid by hyaluronic acid grafted PEI-PLGA nanoparticles for the treatment of triple negative breast cancerShengpeng Wang0Min Shao1Zhangfeng Zhong2Anqi Wang3Jiliang Cao4Yucong Lu5Yitao Wang6Jinming Zhang7Institute of Chinese Medical Sciences, University of MacauZunyi Medical University Zhuhai CampusInstitute of Chinese Medical Sciences, University of MacauInstitute of Chinese Medical Sciences, University of MacauInstitute of Chinese Medical Sciences, University of MacauZunyi Medical University Zhuhai CampusInstitute of Chinese Medical Sciences, University of MacauInstitute of Chinese Medical Sciences, University of MacauTumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-based combination therapy and gene therapy are new strategies to potentially overcome the limitations of TRAIL, however, the lack of efficient and low toxic vectors remains the major obstacle. In this study, we developed a hyaluronic acid (HA)-decorated polyethylenimine-poly(d,l-lactide-co-glycolide) (PEI-PLGA) nanoparticle (NP) system for targeted co-delivery of TRAIL plasmid (pTRAIL) and gambogic acid (GA) in triple-negative breast cancer (TNBC) therapy. GA was encapsulated into the core of the PEI-PLGA NPs while pTRAIL was adsorbed onto the positive NP surface via charge adsorption. The coating of HA on PEI-PLGA NPs functions as a targeting ligand by binding to CD44 receptor of TNBC cells and a shell to neutralize the excess positive charge of inner NPs. The resultant pTRAIL and GA co-loaded HA-coated PEI-PLGA NPs exhibited spherical shape (121.5 nm) and could promote the internalization of loaded cargoes into TNBC cells through the CD44-dependent endocytic pathway. The dual drug-loaded NPs significantly augmented apoptotic cell death in vitro and inhibited TNBC tumor growth in vivo. This multifunctional NP system efficiently co-delivered GA and pTRAIL, thus representing a promising strategy to treat TNBC and bringing forth a platform strategy for co-delivery of therapeutic DNA and chemotherapeutic agents in combinatorial TNBC therapy.http://dx.doi.org/10.1080/10717544.2017.1406558nanoparticlestrailgambogic acidtriple negative breast cancerco-delivery |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shengpeng Wang Min Shao Zhangfeng Zhong Anqi Wang Jiliang Cao Yucong Lu Yitao Wang Jinming Zhang |
spellingShingle |
Shengpeng Wang Min Shao Zhangfeng Zhong Anqi Wang Jiliang Cao Yucong Lu Yitao Wang Jinming Zhang Co-delivery of gambogic acid and TRAIL plasmid by hyaluronic acid grafted PEI-PLGA nanoparticles for the treatment of triple negative breast cancer Drug Delivery nanoparticles trail gambogic acid triple negative breast cancer co-delivery |
author_facet |
Shengpeng Wang Min Shao Zhangfeng Zhong Anqi Wang Jiliang Cao Yucong Lu Yitao Wang Jinming Zhang |
author_sort |
Shengpeng Wang |
title |
Co-delivery of gambogic acid and TRAIL plasmid by hyaluronic acid grafted PEI-PLGA nanoparticles for the treatment of triple negative breast cancer |
title_short |
Co-delivery of gambogic acid and TRAIL plasmid by hyaluronic acid grafted PEI-PLGA nanoparticles for the treatment of triple negative breast cancer |
title_full |
Co-delivery of gambogic acid and TRAIL plasmid by hyaluronic acid grafted PEI-PLGA nanoparticles for the treatment of triple negative breast cancer |
title_fullStr |
Co-delivery of gambogic acid and TRAIL plasmid by hyaluronic acid grafted PEI-PLGA nanoparticles for the treatment of triple negative breast cancer |
title_full_unstemmed |
Co-delivery of gambogic acid and TRAIL plasmid by hyaluronic acid grafted PEI-PLGA nanoparticles for the treatment of triple negative breast cancer |
title_sort |
co-delivery of gambogic acid and trail plasmid by hyaluronic acid grafted pei-plga nanoparticles for the treatment of triple negative breast cancer |
publisher |
Taylor & Francis Group |
series |
Drug Delivery |
issn |
1071-7544 1521-0464 |
publishDate |
2017-01-01 |
description |
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-based combination therapy and gene therapy are new strategies to potentially overcome the limitations of TRAIL, however, the lack of efficient and low toxic vectors remains the major obstacle. In this study, we developed a hyaluronic acid (HA)-decorated polyethylenimine-poly(d,l-lactide-co-glycolide) (PEI-PLGA) nanoparticle (NP) system for targeted co-delivery of TRAIL plasmid (pTRAIL) and gambogic acid (GA) in triple-negative breast cancer (TNBC) therapy. GA was encapsulated into the core of the PEI-PLGA NPs while pTRAIL was adsorbed onto the positive NP surface via charge adsorption. The coating of HA on PEI-PLGA NPs functions as a targeting ligand by binding to CD44 receptor of TNBC cells and a shell to neutralize the excess positive charge of inner NPs. The resultant pTRAIL and GA co-loaded HA-coated PEI-PLGA NPs exhibited spherical shape (121.5 nm) and could promote the internalization of loaded cargoes into TNBC cells through the CD44-dependent endocytic pathway. The dual drug-loaded NPs significantly augmented apoptotic cell death in vitro and inhibited TNBC tumor growth in vivo. This multifunctional NP system efficiently co-delivered GA and pTRAIL, thus representing a promising strategy to treat TNBC and bringing forth a platform strategy for co-delivery of therapeutic DNA and chemotherapeutic agents in combinatorial TNBC therapy. |
topic |
nanoparticles trail gambogic acid triple negative breast cancer co-delivery |
url |
http://dx.doi.org/10.1080/10717544.2017.1406558 |
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