Verification of the <i>Saccharina japonica</i> Translocon <i>Tic20</i> and its Localization in the Chloroplast Membrane in Diatoms
Tic20 is an important translocon protein that plays a role in protein transport in the chloroplast. The sequence of Tic20 was determined in the lower brown alga <i>Saccharina japonica</i>. Structural analysis of SjTic20 revealed a noncanonical structure consisting of an N-terminal non-cy...
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doaj-4fdfb0aa615b4894ad03cf775a0981db2020-11-25T00:50:11ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-08-012016400010.3390/ijms20164000ijms20164000Verification of the <i>Saccharina japonica</i> Translocon <i>Tic20</i> and its Localization in the Chloroplast Membrane in DiatomsZhihang Chen0Xiuliang Wang1Shuang Li2Jianting Yao3Zhanru Shao4Delin Duan5Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaTic20 is an important translocon protein that plays a role in protein transport in the chloroplast. The sequence of Tic20 was determined in the lower brown alga <i>Saccharina japonica</i>. Structural analysis of SjTic20 revealed a noncanonical structure consisting of an N-terminal non-cyanobacterium-originated EF-hand domain (a helix-loop-helix structural domain) and a C-terminal cyanobacterium-originated Tic20 domain. Subcellular localization and transmembrane analysis indicated that SjTic20 featured an “M”-type N<sub>in</sub>-C<sub>in</sub>-terminal orientation, with four transmembrane domains in the innermost membrane of the chloroplast in the microalga <i>Phaeodactylum tricornutum</i>, and the EF-hand domain was entirely extruded into the chloroplast stroma. Our study provides information on the structure, localization, and topological features of SjTic20, and further functional analysis of SjTic20 in <i>S. japonica</i> is needed.https://www.mdpi.com/1422-0067/20/16/4000Tic20EF-hand<i>Phaeodactylum tricornutum</i><i>Saccharina japonica</i>subcellular localizationtransmembrane topology |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhihang Chen Xiuliang Wang Shuang Li Jianting Yao Zhanru Shao Delin Duan |
spellingShingle |
Zhihang Chen Xiuliang Wang Shuang Li Jianting Yao Zhanru Shao Delin Duan Verification of the <i>Saccharina japonica</i> Translocon <i>Tic20</i> and its Localization in the Chloroplast Membrane in Diatoms International Journal of Molecular Sciences Tic20 EF-hand <i>Phaeodactylum tricornutum</i> <i>Saccharina japonica</i> subcellular localization transmembrane topology |
author_facet |
Zhihang Chen Xiuliang Wang Shuang Li Jianting Yao Zhanru Shao Delin Duan |
author_sort |
Zhihang Chen |
title |
Verification of the <i>Saccharina japonica</i> Translocon <i>Tic20</i> and its Localization in the Chloroplast Membrane in Diatoms |
title_short |
Verification of the <i>Saccharina japonica</i> Translocon <i>Tic20</i> and its Localization in the Chloroplast Membrane in Diatoms |
title_full |
Verification of the <i>Saccharina japonica</i> Translocon <i>Tic20</i> and its Localization in the Chloroplast Membrane in Diatoms |
title_fullStr |
Verification of the <i>Saccharina japonica</i> Translocon <i>Tic20</i> and its Localization in the Chloroplast Membrane in Diatoms |
title_full_unstemmed |
Verification of the <i>Saccharina japonica</i> Translocon <i>Tic20</i> and its Localization in the Chloroplast Membrane in Diatoms |
title_sort |
verification of the <i>saccharina japonica</i> translocon <i>tic20</i> and its localization in the chloroplast membrane in diatoms |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-08-01 |
description |
Tic20 is an important translocon protein that plays a role in protein transport in the chloroplast. The sequence of Tic20 was determined in the lower brown alga <i>Saccharina japonica</i>. Structural analysis of SjTic20 revealed a noncanonical structure consisting of an N-terminal non-cyanobacterium-originated EF-hand domain (a helix-loop-helix structural domain) and a C-terminal cyanobacterium-originated Tic20 domain. Subcellular localization and transmembrane analysis indicated that SjTic20 featured an “M”-type N<sub>in</sub>-C<sub>in</sub>-terminal orientation, with four transmembrane domains in the innermost membrane of the chloroplast in the microalga <i>Phaeodactylum tricornutum</i>, and the EF-hand domain was entirely extruded into the chloroplast stroma. Our study provides information on the structure, localization, and topological features of SjTic20, and further functional analysis of SjTic20 in <i>S. japonica</i> is needed. |
topic |
Tic20 EF-hand <i>Phaeodactylum tricornutum</i> <i>Saccharina japonica</i> subcellular localization transmembrane topology |
url |
https://www.mdpi.com/1422-0067/20/16/4000 |
work_keys_str_mv |
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