Molecular surveillance of the antifolate-resistant mutation I164L in imported african isolates of <it>Plasmodium falciparum </it>in Europe: sentinel data from TropNetEurop
<p>Abstract</p> <p>Background</p> <p>Malaria parasites that carry the DHFR-mutation I164L are not only highly resistant to sulfadoxine-pyrimethamine but also to the new antimalarial drug chlorproguanil-dapsone. The spread of this mutation in Africa would result in a pub...
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doaj-4fe3a3df08674f78bf4454f77ed340292020-11-24T21:39:42ZengBMCMalaria Journal1475-28752003-06-01211710.1186/1475-2875-2-17Molecular surveillance of the antifolate-resistant mutation I164L in imported african isolates of <it>Plasmodium falciparum </it>in Europe: sentinel data from TropNetEuropMcWhinney PaulBeran JiøiCunha Saraiva daBurchard GerdSchulze MarcoLaferl HermannMatteelli AlbertoHatz ChristophGascon JoaquimGrobusch Martin PMühlberger NikolaiPeyerl-Hoffmann GabrieleJelinek TomasWichmann OleKollaritsch HerwigKern PeterCuadros JuanAlifrangis MichaelGjørup Ida<p>Abstract</p> <p>Background</p> <p>Malaria parasites that carry the DHFR-mutation I164L are not only highly resistant to sulfadoxine-pyrimethamine but also to the new antimalarial drug chlorproguanil-dapsone. The spread of this mutation in Africa would result in a public health disaster since there is a lack of effective alternatives that are both affordable and safe. Up to now, this mutation has only been described in Asian and Latin-American countries. The objective of this study was to assess the prevalence of this mutation in African isolates of <it>Plasmodium falciparum </it>that have been imported into Europe through travellers.</p> <p>Methods</p> <p>TropNetEurop is a network for the surveillance of travel-associated diseases and seems to cover approximately 12% of all malaria cases imported into Europe. Within this network we screened 277 imported African isolates of <it>P. falciparum </it>with the help of PCR- and enzyme-digestion-methods for the antifolate-resistant mutation I164L.</p> <p>Results</p> <p>The I164L mutation was not detected in any of the isolates tested.</p> <p>Discussion</p> <p>Continuous molecular surveillance of mutations in <it>P. falciparum</it>, as it is practised within TropNetEurop, is an essential tool for the understanding and early detection of the spread of antimalarial drug resistance in Africa.</p> http://www.malariajournal.com/content/2/1/17 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
McWhinney Paul Beran Jiøi Cunha Saraiva da Burchard Gerd Schulze Marco Laferl Hermann Matteelli Alberto Hatz Christoph Gascon Joaquim Grobusch Martin P Mühlberger Nikolai Peyerl-Hoffmann Gabriele Jelinek Tomas Wichmann Ole Kollaritsch Herwig Kern Peter Cuadros Juan Alifrangis Michael Gjørup Ida |
spellingShingle |
McWhinney Paul Beran Jiøi Cunha Saraiva da Burchard Gerd Schulze Marco Laferl Hermann Matteelli Alberto Hatz Christoph Gascon Joaquim Grobusch Martin P Mühlberger Nikolai Peyerl-Hoffmann Gabriele Jelinek Tomas Wichmann Ole Kollaritsch Herwig Kern Peter Cuadros Juan Alifrangis Michael Gjørup Ida Molecular surveillance of the antifolate-resistant mutation I164L in imported african isolates of <it>Plasmodium falciparum </it>in Europe: sentinel data from TropNetEurop Malaria Journal |
author_facet |
McWhinney Paul Beran Jiøi Cunha Saraiva da Burchard Gerd Schulze Marco Laferl Hermann Matteelli Alberto Hatz Christoph Gascon Joaquim Grobusch Martin P Mühlberger Nikolai Peyerl-Hoffmann Gabriele Jelinek Tomas Wichmann Ole Kollaritsch Herwig Kern Peter Cuadros Juan Alifrangis Michael Gjørup Ida |
author_sort |
McWhinney Paul |
title |
Molecular surveillance of the antifolate-resistant mutation I164L in imported african isolates of <it>Plasmodium falciparum </it>in Europe: sentinel data from TropNetEurop |
title_short |
Molecular surveillance of the antifolate-resistant mutation I164L in imported african isolates of <it>Plasmodium falciparum </it>in Europe: sentinel data from TropNetEurop |
title_full |
Molecular surveillance of the antifolate-resistant mutation I164L in imported african isolates of <it>Plasmodium falciparum </it>in Europe: sentinel data from TropNetEurop |
title_fullStr |
Molecular surveillance of the antifolate-resistant mutation I164L in imported african isolates of <it>Plasmodium falciparum </it>in Europe: sentinel data from TropNetEurop |
title_full_unstemmed |
Molecular surveillance of the antifolate-resistant mutation I164L in imported african isolates of <it>Plasmodium falciparum </it>in Europe: sentinel data from TropNetEurop |
title_sort |
molecular surveillance of the antifolate-resistant mutation i164l in imported african isolates of <it>plasmodium falciparum </it>in europe: sentinel data from tropneteurop |
publisher |
BMC |
series |
Malaria Journal |
issn |
1475-2875 |
publishDate |
2003-06-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Malaria parasites that carry the DHFR-mutation I164L are not only highly resistant to sulfadoxine-pyrimethamine but also to the new antimalarial drug chlorproguanil-dapsone. The spread of this mutation in Africa would result in a public health disaster since there is a lack of effective alternatives that are both affordable and safe. Up to now, this mutation has only been described in Asian and Latin-American countries. The objective of this study was to assess the prevalence of this mutation in African isolates of <it>Plasmodium falciparum </it>that have been imported into Europe through travellers.</p> <p>Methods</p> <p>TropNetEurop is a network for the surveillance of travel-associated diseases and seems to cover approximately 12% of all malaria cases imported into Europe. Within this network we screened 277 imported African isolates of <it>P. falciparum </it>with the help of PCR- and enzyme-digestion-methods for the antifolate-resistant mutation I164L.</p> <p>Results</p> <p>The I164L mutation was not detected in any of the isolates tested.</p> <p>Discussion</p> <p>Continuous molecular surveillance of mutations in <it>P. falciparum</it>, as it is practised within TropNetEurop, is an essential tool for the understanding and early detection of the spread of antimalarial drug resistance in Africa.</p> |
url |
http://www.malariajournal.com/content/2/1/17 |
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