CCL2 single nucleotide polymorphism of rs1024611 implicates prominence of inflammatory cascade by univariate modeling in Indian AMD.
The role of chemotactic protein CCL2/MCP-1 has been widely explored in age related macular degeneration (AMD) patients as well as animal models through our previous studies.Aim of the study was to examine the association of another variance of CCL2, rs1024611 in pathophysiology of AMD.This particula...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2018-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC5903598?pdf=render |
id |
doaj-4feef80e6db4471c837925c545c56769 |
---|---|
record_format |
Article |
spelling |
doaj-4feef80e6db4471c837925c545c567692020-11-25T01:46:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01134e019342310.1371/journal.pone.0193423CCL2 single nucleotide polymorphism of rs1024611 implicates prominence of inflammatory cascade by univariate modeling in Indian AMD.Neel Kamal SharmaKaushal SharmaRamandeep SinghSuresh Kumar SharmaAkshay AnandThe role of chemotactic protein CCL2/MCP-1 has been widely explored in age related macular degeneration (AMD) patients as well as animal models through our previous studies.Aim of the study was to examine the association of another variance of CCL2, rs1024611 in pathophysiology of AMD.This particular SNP has been found to be involved in inflammatory processes in various diseases. Total 171 subjects were recruited in the study with all demographic details by administering a standard questionnaire. SNP analysis was performed with TaqMan assay. Linear univariate and ANCOVA modeling was performed to show the interaction of rs1024611 with another SNP variant of CCL-2/CCR-2 (rs4586 and rs1799865) and impact of individual genotypes on CCL-2 expression in the context of AMD pathology.Results showed that both heterozygous (AG, p = 0.01) and homozygous (GG, p = 0.0001) genotypes are associated with AMD pathology. Allele frequency analysis showed that 'G' allele is frequent in AMD patients as compared to controls (p = 0.0001). Moreover, AMD patients who smoke were found to be associated with 'AG' genotype (p = 0.0145). Although, we did not find any significant interaction between the SNP variants by linear univariate analysis but results show the effect of 'CT' genotype on 'TT' genotype in rs4586 by considering rs1024611 as covariate.Based on these results it is imperative that CCL2 mediated pathology may be associated with AMD.http://europepmc.org/articles/PMC5903598?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Neel Kamal Sharma Kaushal Sharma Ramandeep Singh Suresh Kumar Sharma Akshay Anand |
spellingShingle |
Neel Kamal Sharma Kaushal Sharma Ramandeep Singh Suresh Kumar Sharma Akshay Anand CCL2 single nucleotide polymorphism of rs1024611 implicates prominence of inflammatory cascade by univariate modeling in Indian AMD. PLoS ONE |
author_facet |
Neel Kamal Sharma Kaushal Sharma Ramandeep Singh Suresh Kumar Sharma Akshay Anand |
author_sort |
Neel Kamal Sharma |
title |
CCL2 single nucleotide polymorphism of rs1024611 implicates prominence of inflammatory cascade by univariate modeling in Indian AMD. |
title_short |
CCL2 single nucleotide polymorphism of rs1024611 implicates prominence of inflammatory cascade by univariate modeling in Indian AMD. |
title_full |
CCL2 single nucleotide polymorphism of rs1024611 implicates prominence of inflammatory cascade by univariate modeling in Indian AMD. |
title_fullStr |
CCL2 single nucleotide polymorphism of rs1024611 implicates prominence of inflammatory cascade by univariate modeling in Indian AMD. |
title_full_unstemmed |
CCL2 single nucleotide polymorphism of rs1024611 implicates prominence of inflammatory cascade by univariate modeling in Indian AMD. |
title_sort |
ccl2 single nucleotide polymorphism of rs1024611 implicates prominence of inflammatory cascade by univariate modeling in indian amd. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2018-01-01 |
description |
The role of chemotactic protein CCL2/MCP-1 has been widely explored in age related macular degeneration (AMD) patients as well as animal models through our previous studies.Aim of the study was to examine the association of another variance of CCL2, rs1024611 in pathophysiology of AMD.This particular SNP has been found to be involved in inflammatory processes in various diseases. Total 171 subjects were recruited in the study with all demographic details by administering a standard questionnaire. SNP analysis was performed with TaqMan assay. Linear univariate and ANCOVA modeling was performed to show the interaction of rs1024611 with another SNP variant of CCL-2/CCR-2 (rs4586 and rs1799865) and impact of individual genotypes on CCL-2 expression in the context of AMD pathology.Results showed that both heterozygous (AG, p = 0.01) and homozygous (GG, p = 0.0001) genotypes are associated with AMD pathology. Allele frequency analysis showed that 'G' allele is frequent in AMD patients as compared to controls (p = 0.0001). Moreover, AMD patients who smoke were found to be associated with 'AG' genotype (p = 0.0145). Although, we did not find any significant interaction between the SNP variants by linear univariate analysis but results show the effect of 'CT' genotype on 'TT' genotype in rs4586 by considering rs1024611 as covariate.Based on these results it is imperative that CCL2 mediated pathology may be associated with AMD. |
url |
http://europepmc.org/articles/PMC5903598?pdf=render |
work_keys_str_mv |
AT neelkamalsharma ccl2singlenucleotidepolymorphismofrs1024611implicatesprominenceofinflammatorycascadebyunivariatemodelinginindianamd AT kaushalsharma ccl2singlenucleotidepolymorphismofrs1024611implicatesprominenceofinflammatorycascadebyunivariatemodelinginindianamd AT ramandeepsingh ccl2singlenucleotidepolymorphismofrs1024611implicatesprominenceofinflammatorycascadebyunivariatemodelinginindianamd AT sureshkumarsharma ccl2singlenucleotidepolymorphismofrs1024611implicatesprominenceofinflammatorycascadebyunivariatemodelinginindianamd AT akshayanand ccl2singlenucleotidepolymorphismofrs1024611implicatesprominenceofinflammatorycascadebyunivariatemodelinginindianamd |
_version_ |
1725018962189615104 |