Amplification of 16S rRNA gene sequences to differentiate two highly related bradyrhizobia species Amplificação de seqüências do gene RNAr 16S para diferenciar duas espécies de bradirrizóbios altamente relacionadas

Amplification of 16S rRNA gene sequences to differentiate two highly related bradyrhizobia species Amplificação de seqüências do gene RNAr 16S para diferenciar duas espécies de bradirrizóbios altamente relacionadas

A 16S rRNA gene PCR-based assay was developed aiming at a fast molecular diagnostic method to differentiate the two phylogenetically closely related species Bradyrhizobium japonicum and B. elkanii, isolated from soybean nodules, in order to identify those more competitive and comprising greater nitr...

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Bibliographic Details
Main Authors: Adriana Giongo, Adriana Ambrosini, João Ruy Jardim Freire, Maria Helena Bodanese Zanettini, Luciane Maria Pereira Passaglia
Format: Article
Language:English
Published: Embrapa Informação Tecnológica 2007-09-01
Series:Pesquisa Agropecuária Brasileira
Subjects:
PCR
diversidade de rizóbios
inoculantes comerciais
rhizobial diversity
commercial inoculants
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000900019

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