Genetic diversity of Salp15 in the Ixodes ricinus complex (Acari: Ixodidae).
Salp15, a 15-kDa tick salivary gland protein, is both essential for ticks to successfully obtain host blood and also facilitates transmission of Lyme borreliosis. To determine whether the Salp15 gene is expressed in Ixodes persulcatus and Ixodes sinensis, principle vectors of Lyme borreliosis in Chi...
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doaj-5003946102774a189198bef7111a80c62020-11-25T01:34:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9413110.1371/journal.pone.0094131Genetic diversity of Salp15 in the Ixodes ricinus complex (Acari: Ixodidae).Xin WangYong HuangSi-bo NiuBao-Gui JiangNa JiaLeo van der GeestXue-bing NiYi SunWu-Chun CaoSalp15, a 15-kDa tick salivary gland protein, is both essential for ticks to successfully obtain host blood and also facilitates transmission of Lyme borreliosis. To determine whether the Salp15 gene is expressed in Ixodes persulcatus and Ixodes sinensis, principle vectors of Lyme borreliosis in China, we studied transcriptions of this gene in semi-engorged larvae, nymph and adults of these two species. A total of eight Salp15 homologues, five in I. persulcatus and three in I. sinensis, were identified by reverse transcriptase-polymerase chain reaction (RT-PCR). Interestingly, the intra-species similarity of Salp15 is approximately equal to its interspecies similarity and more than one Salp15 protein is expressed in a certain tick developmental stage. Comparison of DNA and proteins with other available tick Salp15 homologues suggests that the Salp15 superfamily is genetically conserved and diverse in the Ixodes ricinus complex. These findings indicate that Salp15 proteins in the I. ricinus complex may play an essential role in interacting with the host immune system and transmission of Borrelia genospecies.http://europepmc.org/articles/PMC3979764?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xin Wang Yong Huang Si-bo Niu Bao-Gui Jiang Na Jia Leo van der Geest Xue-bing Ni Yi Sun Wu-Chun Cao |
spellingShingle |
Xin Wang Yong Huang Si-bo Niu Bao-Gui Jiang Na Jia Leo van der Geest Xue-bing Ni Yi Sun Wu-Chun Cao Genetic diversity of Salp15 in the Ixodes ricinus complex (Acari: Ixodidae). PLoS ONE |
author_facet |
Xin Wang Yong Huang Si-bo Niu Bao-Gui Jiang Na Jia Leo van der Geest Xue-bing Ni Yi Sun Wu-Chun Cao |
author_sort |
Xin Wang |
title |
Genetic diversity of Salp15 in the Ixodes ricinus complex (Acari: Ixodidae). |
title_short |
Genetic diversity of Salp15 in the Ixodes ricinus complex (Acari: Ixodidae). |
title_full |
Genetic diversity of Salp15 in the Ixodes ricinus complex (Acari: Ixodidae). |
title_fullStr |
Genetic diversity of Salp15 in the Ixodes ricinus complex (Acari: Ixodidae). |
title_full_unstemmed |
Genetic diversity of Salp15 in the Ixodes ricinus complex (Acari: Ixodidae). |
title_sort |
genetic diversity of salp15 in the ixodes ricinus complex (acari: ixodidae). |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Salp15, a 15-kDa tick salivary gland protein, is both essential for ticks to successfully obtain host blood and also facilitates transmission of Lyme borreliosis. To determine whether the Salp15 gene is expressed in Ixodes persulcatus and Ixodes sinensis, principle vectors of Lyme borreliosis in China, we studied transcriptions of this gene in semi-engorged larvae, nymph and adults of these two species. A total of eight Salp15 homologues, five in I. persulcatus and three in I. sinensis, were identified by reverse transcriptase-polymerase chain reaction (RT-PCR). Interestingly, the intra-species similarity of Salp15 is approximately equal to its interspecies similarity and more than one Salp15 protein is expressed in a certain tick developmental stage. Comparison of DNA and proteins with other available tick Salp15 homologues suggests that the Salp15 superfamily is genetically conserved and diverse in the Ixodes ricinus complex. These findings indicate that Salp15 proteins in the I. ricinus complex may play an essential role in interacting with the host immune system and transmission of Borrelia genospecies. |
url |
http://europepmc.org/articles/PMC3979764?pdf=render |
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