Human Mast Cell Tryptase Is a Potential Treatment for Snakebite Envenoming Across Multiple Snake Species
Snake envenoming is a serious and neglected public health crisis that is responsible for as many as 125,000 deaths per year, which is one of the reasons the World Health Organization has recently reinstated snakebite envenoming to its list of category A neglected tropical diseases. Here, we investig...
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doaj-500b33f0945e417189240f89184b40d32020-11-24T21:40:39ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-07-01910.3389/fimmu.2018.01532376082Human Mast Cell Tryptase Is a Potential Treatment for Snakebite Envenoming Across Multiple Snake SpeciesElizabeth Anderson0Kathrin Stavenhagen1Daniel Kolarich2Christian P. Sommerhoff3Marcus Maurer4Martin Metz5Department of Dermatology and Allergy, Charité – Universitätsmedizin Berlin, Berlin, GermanyDepartment of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, GermanyDepartment of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, GermanyInstitute of Laboratory Medicine, University Hospital, Ludwig-Maximilians-University, Munich, GermanyDepartment of Dermatology and Allergy, Charité – Universitätsmedizin Berlin, Berlin, GermanyDepartment of Dermatology and Allergy, Charité – Universitätsmedizin Berlin, Berlin, GermanySnake envenoming is a serious and neglected public health crisis that is responsible for as many as 125,000 deaths per year, which is one of the reasons the World Health Organization has recently reinstated snakebite envenoming to its list of category A neglected tropical diseases. Here, we investigated the ability of human mast cell proteases to detoxify six venoms from a spectrum of phylogenetically distinct snakes. To this end, we developed a zebrafish model to assess effects on the toxicity of the venoms and characterized the degradation of venom proteins by mass spectrometry. All snake venoms tested were detoxified by degradation of various venom proteins by the mast cell protease tryptase β, and not by other proteases. Our data show that recombinant human tryptase β degrades and detoxifies a phylogenetically wide range of venoms, indicating that recombinant human tryptase could possibly be developed as a universal antidote to venomous snakebites.https://www.frontiersin.org/article/10.3389/fimmu.2018.01532/fullmast cellvenomproteasessnakesantivenom |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elizabeth Anderson Kathrin Stavenhagen Daniel Kolarich Christian P. Sommerhoff Marcus Maurer Martin Metz |
spellingShingle |
Elizabeth Anderson Kathrin Stavenhagen Daniel Kolarich Christian P. Sommerhoff Marcus Maurer Martin Metz Human Mast Cell Tryptase Is a Potential Treatment for Snakebite Envenoming Across Multiple Snake Species Frontiers in Immunology mast cell venom proteases snakes antivenom |
author_facet |
Elizabeth Anderson Kathrin Stavenhagen Daniel Kolarich Christian P. Sommerhoff Marcus Maurer Martin Metz |
author_sort |
Elizabeth Anderson |
title |
Human Mast Cell Tryptase Is a Potential Treatment for Snakebite Envenoming Across Multiple Snake Species |
title_short |
Human Mast Cell Tryptase Is a Potential Treatment for Snakebite Envenoming Across Multiple Snake Species |
title_full |
Human Mast Cell Tryptase Is a Potential Treatment for Snakebite Envenoming Across Multiple Snake Species |
title_fullStr |
Human Mast Cell Tryptase Is a Potential Treatment for Snakebite Envenoming Across Multiple Snake Species |
title_full_unstemmed |
Human Mast Cell Tryptase Is a Potential Treatment for Snakebite Envenoming Across Multiple Snake Species |
title_sort |
human mast cell tryptase is a potential treatment for snakebite envenoming across multiple snake species |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2018-07-01 |
description |
Snake envenoming is a serious and neglected public health crisis that is responsible for as many as 125,000 deaths per year, which is one of the reasons the World Health Organization has recently reinstated snakebite envenoming to its list of category A neglected tropical diseases. Here, we investigated the ability of human mast cell proteases to detoxify six venoms from a spectrum of phylogenetically distinct snakes. To this end, we developed a zebrafish model to assess effects on the toxicity of the venoms and characterized the degradation of venom proteins by mass spectrometry. All snake venoms tested were detoxified by degradation of various venom proteins by the mast cell protease tryptase β, and not by other proteases. Our data show that recombinant human tryptase β degrades and detoxifies a phylogenetically wide range of venoms, indicating that recombinant human tryptase could possibly be developed as a universal antidote to venomous snakebites. |
topic |
mast cell venom proteases snakes antivenom |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2018.01532/full |
work_keys_str_mv |
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