Trypanosoma spp. Neobats: Insights about those poorly known trypanosomatids
Bats are infected with several trypanosomatid species; however, assessing the diversity of this interaction remains challenging since there are species apparently unable to grow in conventional culture media. Accordingly, the ecology and biology of the Molecular Operational Taxonomic Units (MOTUs) T...
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doaj-502b681d23cd41349065b33bbd4169b62021-09-17T04:36:08ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442021-12-0116145152Trypanosoma spp. Neobats: Insights about those poorly known trypanosomatidsFernanda Moreira Alves0Diana Azeredo Rangel1Emmanuel Messias Vilar2Márcio Galvão Pavan3Ricardo Moratelli4André Luiz Rodrigues Roque5Ana Maria Jansen6Laboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, Brazil; Post-Graduate Program in Parasite Biology, Oswaldo Cruz Institute, Rio de Janeiro, Rio de Janeiro, Brazil; Corresponding author. Laboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Av. Brasil 4365, 21040-900, Rio de Janeiro, RJ, Brazil.Laboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, Brazil; Post-Graduate Program in Parasite Biology, Oswaldo Cruz Institute, Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Mammals, Department of Systematics and Ecology, Federal University of Paraíba, João Pessoa, Paraíba, BrazilLaboratory of Hematozoan-Transmitting Mosquitoes, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, BrazilFiocruz Mata Atlântica, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, BrazilBats are infected with several trypanosomatid species; however, assessing the diversity of this interaction remains challenging since there are species apparently unable to grow in conventional culture media. Accordingly, the ecology and biology of the Molecular Operational Taxonomic Units (MOTUs) Trypanosoma spp. Neobats are unknown. Therefore, we performed the molecular characterization targeting the 18S small subunit rDNA from the blood clot of 280 bats of three Brazilian regions (Paraíba, Rio de Janeiro and Acre states), bypassing the selective pressure of hemoculture. From 68 (24%) positive blood clot samples, we obtained 49 satisfactory sequences. Of these successfully sequenced results, T. spp. Neobats (1, 3 and 4) represented 67%, with the most abundant T. sp. Neobat 4 (53%). Our results show: (1) high abundance and wide geographic range of T. sp. Neobat 4, restricted to Carollia bats; (2) high infection rate of T. sp. Neobat 4 in Carollia perspicillata populations (mean 26%); (3) infection with the monoxenous Crithidia mellificae; and (4) a new MOTU (T. sp. Neobat 5) in Artibeus cinereus, positioning in the Trypanosoma wauwau clade. These data corroborate the importance of bats as hosts of many Trypanosoma species and C. mellificae. They also show that the diversity of the T. wauwau clade is underestimated and warn about the high magnitude of trypanosomes we overpass with the hemoculture. Our findings combined with previous data show that T. spp. Neobats include host-specific and host-generalist species, probably playing different ecological roles: T. sp. Neobat 1 shows broad host range; T. spp. Neobat 3 and 4 are restricted to Artibeus and Carollia, respectively. Finally, T. Neobat 4 seems to be a well-succeeded parasite, especially within C. perspicillata metapopulations across a wide geographical distribution. This work is a step forward to understand the biology and life history of T. spp. Neobats.http://www.sciencedirect.com/science/article/pii/S2213224421000961TrypanosomaNeobatMOTUCrithidiaBatsBlood clot |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fernanda Moreira Alves Diana Azeredo Rangel Emmanuel Messias Vilar Márcio Galvão Pavan Ricardo Moratelli André Luiz Rodrigues Roque Ana Maria Jansen |
spellingShingle |
Fernanda Moreira Alves Diana Azeredo Rangel Emmanuel Messias Vilar Márcio Galvão Pavan Ricardo Moratelli André Luiz Rodrigues Roque Ana Maria Jansen Trypanosoma spp. Neobats: Insights about those poorly known trypanosomatids International Journal for Parasitology: Parasites and Wildlife Trypanosoma Neobat MOTU Crithidia Bats Blood clot |
author_facet |
Fernanda Moreira Alves Diana Azeredo Rangel Emmanuel Messias Vilar Márcio Galvão Pavan Ricardo Moratelli André Luiz Rodrigues Roque Ana Maria Jansen |
author_sort |
Fernanda Moreira Alves |
title |
Trypanosoma spp. Neobats: Insights about those poorly known trypanosomatids |
title_short |
Trypanosoma spp. Neobats: Insights about those poorly known trypanosomatids |
title_full |
Trypanosoma spp. Neobats: Insights about those poorly known trypanosomatids |
title_fullStr |
Trypanosoma spp. Neobats: Insights about those poorly known trypanosomatids |
title_full_unstemmed |
Trypanosoma spp. Neobats: Insights about those poorly known trypanosomatids |
title_sort |
trypanosoma spp. neobats: insights about those poorly known trypanosomatids |
publisher |
Elsevier |
series |
International Journal for Parasitology: Parasites and Wildlife |
issn |
2213-2244 |
publishDate |
2021-12-01 |
description |
Bats are infected with several trypanosomatid species; however, assessing the diversity of this interaction remains challenging since there are species apparently unable to grow in conventional culture media. Accordingly, the ecology and biology of the Molecular Operational Taxonomic Units (MOTUs) Trypanosoma spp. Neobats are unknown. Therefore, we performed the molecular characterization targeting the 18S small subunit rDNA from the blood clot of 280 bats of three Brazilian regions (Paraíba, Rio de Janeiro and Acre states), bypassing the selective pressure of hemoculture. From 68 (24%) positive blood clot samples, we obtained 49 satisfactory sequences. Of these successfully sequenced results, T. spp. Neobats (1, 3 and 4) represented 67%, with the most abundant T. sp. Neobat 4 (53%). Our results show: (1) high abundance and wide geographic range of T. sp. Neobat 4, restricted to Carollia bats; (2) high infection rate of T. sp. Neobat 4 in Carollia perspicillata populations (mean 26%); (3) infection with the monoxenous Crithidia mellificae; and (4) a new MOTU (T. sp. Neobat 5) in Artibeus cinereus, positioning in the Trypanosoma wauwau clade. These data corroborate the importance of bats as hosts of many Trypanosoma species and C. mellificae. They also show that the diversity of the T. wauwau clade is underestimated and warn about the high magnitude of trypanosomes we overpass with the hemoculture. Our findings combined with previous data show that T. spp. Neobats include host-specific and host-generalist species, probably playing different ecological roles: T. sp. Neobat 1 shows broad host range; T. spp. Neobat 3 and 4 are restricted to Artibeus and Carollia, respectively. Finally, T. Neobat 4 seems to be a well-succeeded parasite, especially within C. perspicillata metapopulations across a wide geographical distribution. This work is a step forward to understand the biology and life history of T. spp. Neobats. |
topic |
Trypanosoma Neobat MOTU Crithidia Bats Blood clot |
url |
http://www.sciencedirect.com/science/article/pii/S2213224421000961 |
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