Plastination of decalcified bone by a new resin technique
Background: The scope of this study was to preserve whole detailed structure of dissected and decalcified bones, taken from used cadavers, by a new plastination technique. Materials and Methods: Specimens we used in this study were sheep femurs and human bones including pelvis, femur, tibia, and fi...
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Wolters Kluwer Medknow Publications
2014-01-01
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doaj-5045c1f70b17470d9e3bbfbedd6fd0a82020-11-24T23:13:52ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752277-91752014-01-0131181810.4103/2277-9175.124648Plastination of decalcified bone by a new resin techniqueAbbas Ali RabieiEbrahim EsfandiaryMorteza HajianAtefe ShamosiMohammad MardaniBahman RashidiMohsen SetayeshmehrBackground: The scope of this study was to preserve whole detailed structure of dissected and decalcified bones, taken from used cadavers, by a new plastination technique. Materials and Methods: Specimens we used in this study were sheep femurs and human bones including pelvis, femur, tibia, and fibula. Bones, at first, fixed with 5% formalin and were decalcified with 5% nitric acid, and then were fixed again and washed under the tap water. The resulted flexible bones were dehydrated in -25C acetone and degreased them in +25C acetone. Then, the experimental and control specimen were placed in the vacuum chamber for forced impregnation with our new flexible unsaturated polyester resin (UP 89 method) and silicon resin (S 10 method), respectively. Finally, the strength and flexibility of plastinated decalcified specimens were investigated by tensometer, and the weight diversity was measured by digital balance. Results: Plastinated bones prepared by this technique were found to be dried, non-fragile, durable, odorless, non-greasy, and demonstrating all detailed structures of the bones. Tensile and weight tests results indicated that plastinated decalcified femurs have owned higher flexibility and strength but lesser weight than plastinated undecalcified femurs. The characteristics of both experimental and control groups of plastinated decalcified specimens were found to have no significant difference. Conclusions: Our synthesized resin found to be much more economical than conventional plastination method. In more details, properties of these new products were the same as, S 10 method, from points of strength, flexibility and weight, but, since the money cost for producing them was about one fifth that of S 10 method.http://www.advbiores.net/article.asp?issn=2277-9175;year=2014;volume=3;issue=1;spage=18;epage=18;aulast=RabieiBonesdecalcificationplastinationUP 89 method |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Abbas Ali Rabiei Ebrahim Esfandiary Morteza Hajian Atefe Shamosi Mohammad Mardani Bahman Rashidi Mohsen Setayeshmehr |
spellingShingle |
Abbas Ali Rabiei Ebrahim Esfandiary Morteza Hajian Atefe Shamosi Mohammad Mardani Bahman Rashidi Mohsen Setayeshmehr Plastination of decalcified bone by a new resin technique Advanced Biomedical Research Bones decalcification plastination UP 89 method |
author_facet |
Abbas Ali Rabiei Ebrahim Esfandiary Morteza Hajian Atefe Shamosi Mohammad Mardani Bahman Rashidi Mohsen Setayeshmehr |
author_sort |
Abbas Ali Rabiei |
title |
Plastination of decalcified bone by a new resin technique |
title_short |
Plastination of decalcified bone by a new resin technique |
title_full |
Plastination of decalcified bone by a new resin technique |
title_fullStr |
Plastination of decalcified bone by a new resin technique |
title_full_unstemmed |
Plastination of decalcified bone by a new resin technique |
title_sort |
plastination of decalcified bone by a new resin technique |
publisher |
Wolters Kluwer Medknow Publications |
series |
Advanced Biomedical Research |
issn |
2277-9175 2277-9175 |
publishDate |
2014-01-01 |
description |
Background: The scope of this study was to preserve whole detailed structure of dissected and decalcified bones, taken from used cadavers, by a new plastination technique.
Materials and Methods: Specimens we used in this study were sheep femurs and human bones including pelvis, femur, tibia, and fibula. Bones, at first, fixed with 5% formalin and were decalcified with 5% nitric acid, and then were fixed again and washed under the tap water. The resulted flexible bones were dehydrated in -25C acetone and degreased them in +25C acetone. Then, the experimental and control specimen were placed in the vacuum chamber for forced impregnation with our new flexible unsaturated polyester resin (UP 89 method) and silicon resin (S 10 method), respectively. Finally, the strength and flexibility of plastinated decalcified specimens were investigated by tensometer, and the weight diversity was measured by digital balance.
Results: Plastinated bones prepared by this technique were found to be dried, non-fragile, durable, odorless, non-greasy, and demonstrating all detailed structures of the bones. Tensile and weight tests results indicated that plastinated decalcified femurs have owned higher flexibility and strength but lesser weight than plastinated undecalcified femurs. The characteristics of both experimental and control groups of plastinated decalcified specimens were found to have no significant difference.
Conclusions: Our synthesized resin found to be much more economical than conventional plastination method. In more details, properties of these new products were the same as, S 10 method, from points of strength, flexibility and weight, but, since the money cost for producing them was about one fifth that of S 10 method. |
topic |
Bones decalcification plastination UP 89 method |
url |
http://www.advbiores.net/article.asp?issn=2277-9175;year=2014;volume=3;issue=1;spage=18;epage=18;aulast=Rabiei |
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