Supplementation of Hermetia illucens Larvae in Poultry By-Product Meal-Based Barramundi, Lates calcarifer Diets Improves Adipocyte Cell Size, Skin Barrier Functions, and Immune Responses
A 6-week feeding trial was performed to examine the effects of supplementing Hermetia illucens (HI) larvae meal when fishmeal (FM) was replaced with poultry by-product meal (PBM) in juvenile barramundi, Lates calcarifer diet. The effect was evaluated in terms of barramundi growth, filet quality, int...
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doaj-50468a917cff46e79b4f2a2469640a2e2021-01-14T05:18:23ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2021-01-01710.3389/fnut.2020.613158613158Supplementation of Hermetia illucens Larvae in Poultry By-Product Meal-Based Barramundi, Lates calcarifer Diets Improves Adipocyte Cell Size, Skin Barrier Functions, and Immune ResponsesMd Reaz Chaklader0Janet Howieson1Ravi Fotedar2Muhammad A. B. Siddik3School of Molecular and Life Sciences, Curtin University, Bentley, WA, AustraliaSchool of Molecular and Life Sciences, Curtin University, Bentley, WA, AustraliaSchool of Molecular and Life Sciences, Curtin University, Bentley, WA, AustraliaDepartment of Fisheries Biology & Genetics, Faculty of Fisheries, Patuakhali Science and Technology University, Patuakhali, BangladeshA 6-week feeding trial was performed to examine the effects of supplementing Hermetia illucens (HI) larvae meal when fishmeal (FM) was replaced with poultry by-product meal (PBM) in juvenile barramundi, Lates calcarifer diet. The effect was evaluated in terms of barramundi growth, filet quality, internal tissue structure, serum biochemistry, skin neutral mucins, immune response, and resistance to Vibrio harveyi. Three isonitrogenous (48% crude protein) and isolipidic (18% crude lipid) diets: an FM-based diet (control) and two diets containing 60 and 75% of PBM supplemented with 10% HI larvae (60PBM + HI and 75PBM + HI) were formulated. A total of 225 barramundi, with an average weight of 15.87 ± 0.14 g, were randomly distributed into nine tanks, each holding 25 fish. There were no significant effects of test diets on growth, but feeding HI-supplemented PBM diets significantly increased the survival rate. A significantly reduced intraperitoneal fat index in HI-supplemented-PBM-fed fish was correlated to a decreased size of peritoneal adipocytes. The observation of no histopathological alteration of the liver in the HI-supplemented-PBM-fed fish was further supported by significant alterations in serum biochemistry, in particular, a decreasing tendency of alanine transaminase, glutamate dehydrogenase, and total bilirubin. A 14-day challenge with V. harveyi indicated that HI-supplemented PBM diets reduced the infection rate in barramundi. After 24 h of infection, increased serum (lysozyme) and skin barrier functions, down-regulation of interleukin-1beta, and upregulation of interleukin-10 were found in HI-supplemented-PBM-fed fish.https://www.frontiersin.org/articles/10.3389/fnut.2020.613158/fullVibrio harveyiinsect larvaeadipocyte cellneutral mucinsimmune functionbarramundi |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Md Reaz Chaklader Janet Howieson Ravi Fotedar Muhammad A. B. Siddik |
spellingShingle |
Md Reaz Chaklader Janet Howieson Ravi Fotedar Muhammad A. B. Siddik Supplementation of Hermetia illucens Larvae in Poultry By-Product Meal-Based Barramundi, Lates calcarifer Diets Improves Adipocyte Cell Size, Skin Barrier Functions, and Immune Responses Frontiers in Nutrition Vibrio harveyi insect larvae adipocyte cell neutral mucins immune function barramundi |
author_facet |
Md Reaz Chaklader Janet Howieson Ravi Fotedar Muhammad A. B. Siddik |
author_sort |
Md Reaz Chaklader |
title |
Supplementation of Hermetia illucens Larvae in Poultry By-Product Meal-Based Barramundi, Lates calcarifer Diets Improves Adipocyte Cell Size, Skin Barrier Functions, and Immune Responses |
title_short |
Supplementation of Hermetia illucens Larvae in Poultry By-Product Meal-Based Barramundi, Lates calcarifer Diets Improves Adipocyte Cell Size, Skin Barrier Functions, and Immune Responses |
title_full |
Supplementation of Hermetia illucens Larvae in Poultry By-Product Meal-Based Barramundi, Lates calcarifer Diets Improves Adipocyte Cell Size, Skin Barrier Functions, and Immune Responses |
title_fullStr |
Supplementation of Hermetia illucens Larvae in Poultry By-Product Meal-Based Barramundi, Lates calcarifer Diets Improves Adipocyte Cell Size, Skin Barrier Functions, and Immune Responses |
title_full_unstemmed |
Supplementation of Hermetia illucens Larvae in Poultry By-Product Meal-Based Barramundi, Lates calcarifer Diets Improves Adipocyte Cell Size, Skin Barrier Functions, and Immune Responses |
title_sort |
supplementation of hermetia illucens larvae in poultry by-product meal-based barramundi, lates calcarifer diets improves adipocyte cell size, skin barrier functions, and immune responses |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Nutrition |
issn |
2296-861X |
publishDate |
2021-01-01 |
description |
A 6-week feeding trial was performed to examine the effects of supplementing Hermetia illucens (HI) larvae meal when fishmeal (FM) was replaced with poultry by-product meal (PBM) in juvenile barramundi, Lates calcarifer diet. The effect was evaluated in terms of barramundi growth, filet quality, internal tissue structure, serum biochemistry, skin neutral mucins, immune response, and resistance to Vibrio harveyi. Three isonitrogenous (48% crude protein) and isolipidic (18% crude lipid) diets: an FM-based diet (control) and two diets containing 60 and 75% of PBM supplemented with 10% HI larvae (60PBM + HI and 75PBM + HI) were formulated. A total of 225 barramundi, with an average weight of 15.87 ± 0.14 g, were randomly distributed into nine tanks, each holding 25 fish. There were no significant effects of test diets on growth, but feeding HI-supplemented PBM diets significantly increased the survival rate. A significantly reduced intraperitoneal fat index in HI-supplemented-PBM-fed fish was correlated to a decreased size of peritoneal adipocytes. The observation of no histopathological alteration of the liver in the HI-supplemented-PBM-fed fish was further supported by significant alterations in serum biochemistry, in particular, a decreasing tendency of alanine transaminase, glutamate dehydrogenase, and total bilirubin. A 14-day challenge with V. harveyi indicated that HI-supplemented PBM diets reduced the infection rate in barramundi. After 24 h of infection, increased serum (lysozyme) and skin barrier functions, down-regulation of interleukin-1beta, and upregulation of interleukin-10 were found in HI-supplemented-PBM-fed fish. |
topic |
Vibrio harveyi insect larvae adipocyte cell neutral mucins immune function barramundi |
url |
https://www.frontiersin.org/articles/10.3389/fnut.2020.613158/full |
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