Hemocytes of zebra mussels (Dreissena polymorpha) are relevant cells for the monitoring of environmental genotoxicity by the comet assay.

• Main Authors: Marc Bonnard, Sandrine Joachim
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2015-06-01
• Series: Frontiers in Genetics
• Subjects: Carbamazepine; Hemocytes; mesocosm; confounding factors; Zebra mussels
• Online Access: http://journal.frontiersin.org/Journal/10.3389/conf.fgene.2015.01.00065/full

The measure of DNA integrity by the single cell gel electrophoresis (SCGE) or comet assay is especially recommended for its sensitivity and its capacity for detecting different types of damages. Therefore, it has been applied in environmental genotoxicity in a variety of organisms. It appears today necessary to define both reference and threshold levels of DNA damage, for their application in in situ biomonitoring. However, little is known about the influence of both biological (sex, reproduction status) or external (temperature…) confounding factors on the measure of DNA damage by the comet assay. These variables need to be taken into account if the robustness of the assay is to be established (Jha, 2008). In the zebra mussel Dreissena polymorpha (recommended as a sentinel species in the evaluation of freshwater quality) the measure of DNA damage by the comet assay is mainly performed on hemocytes, which are circulating cells involved in key physiological functions such as immunity, homeostasis, detoxication…. This communication will present and discuss results from an innovative study about the variability of the baseline level of DNA damage in hemocytes of mussels encaged for one year in the canal de l’Aisne à la Marne (Reims), according to their sex and their reproductive status. The sensitivity and the suitability of hemocytes in the evaluation of environmental genotoxicity will also be discussed, referring to observations during a 6 month-exposure of mussels in mesocosms to environmentally realistic concentrations of carbamazepine.