Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil
We evaluated the effectiveness of serological and parasitological methods for cutaneous leishmaniasis (CL) diagnosis in patients from the central region of Paraná state, southern Brazil. Five groups were compared: clinical diagnosis, parasitological diagnosis, communicants, inhabitants of a non-ende...
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doaj-50746c179cc5427787da0a5a56292ebb2020-11-25T03:06:26ZengElsevierBrazilian Journal of Infectious Diseases1678-4391131475210.1590/S1413-86702009000100011S1413-86702009000100011Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, BrazilRegiane Szargiki0Edilene Alcântara de Castro1Ennio Luz2Wolodymir Kowalthuk3Ângela Maria Machado4Vanete Thomaz-Soccol5SESAUniversidade Federal do ParanáUniversidade Federal do ParanáPrefeitura Municipal de PrudentópolisPrefeitura Municipal de PrudentópolisUniversidade Federal do ParanáWe evaluated the effectiveness of serological and parasitological methods for cutaneous leishmaniasis (CL) diagnosis in patients from the central region of Paraná state, southern Brazil. Five groups were compared: clinical diagnosis, parasitological diagnosis, communicants, inhabitants of a non-endemic area and carriers of other etiologies. Two antigens were prepared from promastigotes of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis for indirect immunofluorescence assay, ELISA and immunoblotting. The parasitological approaches detected 79.3% of the patients with a clinical diagnosis; the parasites were identified by PCR as L. (V.) braziliensis. Serological methods showed 95% sensitivity for homologous antigens. Immunoblotting revealed specific proteins for diagnosis of CL and detected 96.6% of the patients when L. (V.) braziliensis was used as an antigen, and 83.3% with L. (L.) amazonensis. This study demonstrated the importance of differential diagnosis for leishmaniasis; the association of two or more indirect methods increased diagnosis sensitivity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100011&lng=en&tlng=enCutaneous leishmaniasisimmunodiagnosisantigenwestern blotting |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Regiane Szargiki Edilene Alcântara de Castro Ennio Luz Wolodymir Kowalthuk Ângela Maria Machado Vanete Thomaz-Soccol |
spellingShingle |
Regiane Szargiki Edilene Alcântara de Castro Ennio Luz Wolodymir Kowalthuk Ângela Maria Machado Vanete Thomaz-Soccol Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil Brazilian Journal of Infectious Diseases Cutaneous leishmaniasis immunodiagnosis antigen western blotting |
author_facet |
Regiane Szargiki Edilene Alcântara de Castro Ennio Luz Wolodymir Kowalthuk Ângela Maria Machado Vanete Thomaz-Soccol |
author_sort |
Regiane Szargiki |
title |
Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil |
title_short |
Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil |
title_full |
Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil |
title_fullStr |
Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil |
title_full_unstemmed |
Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil |
title_sort |
comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of paraná, brazil |
publisher |
Elsevier |
series |
Brazilian Journal of Infectious Diseases |
issn |
1678-4391 |
description |
We evaluated the effectiveness of serological and parasitological methods for cutaneous leishmaniasis (CL) diagnosis in patients from the central region of Paraná state, southern Brazil. Five groups were compared: clinical diagnosis, parasitological diagnosis, communicants, inhabitants of a non-endemic area and carriers of other etiologies. Two antigens were prepared from promastigotes of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis for indirect immunofluorescence assay, ELISA and immunoblotting. The parasitological approaches detected 79.3% of the patients with a clinical diagnosis; the parasites were identified by PCR as L. (V.) braziliensis. Serological methods showed 95% sensitivity for homologous antigens. Immunoblotting revealed specific proteins for diagnosis of CL and detected 96.6% of the patients when L. (V.) braziliensis was used as an antigen, and 83.3% with L. (L.) amazonensis. This study demonstrated the importance of differential diagnosis for leishmaniasis; the association of two or more indirect methods increased diagnosis sensitivity. |
topic |
Cutaneous leishmaniasis immunodiagnosis antigen western blotting |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100011&lng=en&tlng=en |
work_keys_str_mv |
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