Genomic DNA Methylation Changes in NYGGF4-Overexpression 3T3-L1 Adipocytes
NYGGF4, an obesity-related gene, is proposed to be involved in the development of insulin resistance; however, the underlying molecular mechanisms remain unclear. In the present analysis, NimbleGen tiling arrays were used to determine the patterns of genomic DNA methylation at CpG islands and promot...
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doaj-508fb57bfc2645a2b86e04842cf6e7902020-11-24T23:53:57ZengMDPI AGInternational Journal of Molecular Sciences1422-00672012-11-011312155751558710.3390/ijms131215575Genomic DNA Methylation Changes in NYGGF4-Overexpression 3T3-L1 AdipocytesLei YangMei-Ling TongXia ChiMin ZhangChun-Mei ZhangXi-Rong GuoNYGGF4, an obesity-related gene, is proposed to be involved in the development of insulin resistance; however, the underlying molecular mechanisms remain unclear. In the present analysis, NimbleGen tiling arrays were used to determine the patterns of genomic DNA methylation at CpG islands and promoters in NYGGF4-overexpression adipocytes. A total of 2352 CpG dinucleotides in 2018 genes and 3490 CpG dinucleotides in 3064 genes were found to be hypermethylated or hypomethylated, respectively, in NYGGF4-overexpression adipocytes. Furthermore, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis revealed enrichment of biological processes associated with energy metabolism and signal transduction events, including the peroxisome proliferator-activated receptor gamma (PPARγ) signaling pathway, and mitogen-activated protein kinases(MAPK) and Ras homolog gene family, member A (RhoA) signaling. These data demonstrate that differentially methylated genes are significantly overrepresented in NYGGF4-overexpression adipocytes, providing valuable clues for further exploration of the role of NYGGF4 in insulin sensitivity regulation.http://www.mdpi.com/1422-0067/13/12/15575NYGGF4DNA methylationinsulin resistanceadipocytes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lei Yang Mei-Ling Tong Xia Chi Min Zhang Chun-Mei Zhang Xi-Rong Guo |
spellingShingle |
Lei Yang Mei-Ling Tong Xia Chi Min Zhang Chun-Mei Zhang Xi-Rong Guo Genomic DNA Methylation Changes in NYGGF4-Overexpression 3T3-L1 Adipocytes International Journal of Molecular Sciences NYGGF4 DNA methylation insulin resistance adipocytes |
author_facet |
Lei Yang Mei-Ling Tong Xia Chi Min Zhang Chun-Mei Zhang Xi-Rong Guo |
author_sort |
Lei Yang |
title |
Genomic DNA Methylation Changes in NYGGF4-Overexpression 3T3-L1 Adipocytes |
title_short |
Genomic DNA Methylation Changes in NYGGF4-Overexpression 3T3-L1 Adipocytes |
title_full |
Genomic DNA Methylation Changes in NYGGF4-Overexpression 3T3-L1 Adipocytes |
title_fullStr |
Genomic DNA Methylation Changes in NYGGF4-Overexpression 3T3-L1 Adipocytes |
title_full_unstemmed |
Genomic DNA Methylation Changes in NYGGF4-Overexpression 3T3-L1 Adipocytes |
title_sort |
genomic dna methylation changes in nyggf4-overexpression 3t3-l1 adipocytes |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2012-11-01 |
description |
NYGGF4, an obesity-related gene, is proposed to be involved in the development of insulin resistance; however, the underlying molecular mechanisms remain unclear. In the present analysis, NimbleGen tiling arrays were used to determine the patterns of genomic DNA methylation at CpG islands and promoters in NYGGF4-overexpression adipocytes. A total of 2352 CpG dinucleotides in 2018 genes and 3490 CpG dinucleotides in 3064 genes were found to be hypermethylated or hypomethylated, respectively, in NYGGF4-overexpression adipocytes. Furthermore, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis revealed enrichment of biological processes associated with energy metabolism and signal transduction events, including the peroxisome proliferator-activated receptor gamma (PPARγ) signaling pathway, and mitogen-activated protein kinases(MAPK) and Ras homolog gene family, member A (RhoA) signaling. These data demonstrate that differentially methylated genes are significantly overrepresented in NYGGF4-overexpression adipocytes, providing valuable clues for further exploration of the role of NYGGF4 in insulin sensitivity regulation. |
topic |
NYGGF4 DNA methylation insulin resistance adipocytes |
url |
http://www.mdpi.com/1422-0067/13/12/15575 |
work_keys_str_mv |
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