Anti-Stem Cell Property of Pterostilbene in Gastrointestinal Cancer Cells
Pterostilbene (PTE) is a natural sterbenoid contained in blueberries that has an antioxidant effect. In contrast, PTE also generates oxidative stress in cancer cells and provides an antitumor effect. Here, we examined the potential mechanism of this contrasting effect of PTE using three gastrointest...
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doaj-50904da274994bb5a0670c7842efc0242020-12-09T00:02:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-12-01219347934710.3390/ijms21249347Anti-Stem Cell Property of Pterostilbene in Gastrointestinal Cancer CellsShiori Mori0Shingo Kishi1Kanya Honoki2Rina Fujiwara-Tani3Takuma Moriguchi4Takamitsu Sasaki5Kiyomu Fujii6Shinji Tsukamoto7Hiromasa Fujii8Akira Kido9Yasuhito Tanaka10Yi Luo11Hiroki Kuniyasu12Department of Molecular Pathology, Nara Medical University, 840 Shijo-cho, Kashihara 634-8521, Nara, JapanDepartment of Molecular Pathology, Nara Medical University, 840 Shijo-cho, Kashihara 634-8521, Nara, JapanDepartment of Orthopedics, Nara Medical University, 840 Shijo-cho, Kashihara 634-8522, Nara, JapanDepartment of Molecular Pathology, Nara Medical University, 840 Shijo-cho, Kashihara 634-8521, Nara, JapanDepartment of Molecular Pathology, Nara Medical University, 840 Shijo-cho, Kashihara 634-8521, Nara, JapanDepartment of Molecular Pathology, Nara Medical University, 840 Shijo-cho, Kashihara 634-8521, Nara, JapanDepartment of Molecular Pathology, Nara Medical University, 840 Shijo-cho, Kashihara 634-8521, Nara, JapanDepartment of Orthopedics, Nara Medical University, 840 Shijo-cho, Kashihara 634-8522, Nara, JapanDepartment of Orthopedics, Nara Medical University, 840 Shijo-cho, Kashihara 634-8522, Nara, JapanDepartment of Orthopedics, Nara Medical University, 840 Shijo-cho, Kashihara 634-8522, Nara, JapanDepartment of Orthopedics, Nara Medical University, 840 Shijo-cho, Kashihara 634-8522, Nara, JapanDepartment of Molecular Pathology, Nara Medical University, 840 Shijo-cho, Kashihara 634-8521, Nara, JapanDepartment of Molecular Pathology, Nara Medical University, 840 Shijo-cho, Kashihara 634-8521, Nara, JapanPterostilbene (PTE) is a natural sterbenoid contained in blueberries that has an antioxidant effect. In contrast, PTE also generates oxidative stress in cancer cells and provides an antitumor effect. Here, we examined the potential mechanism of this contrasting effect of PTE using three gastrointestinal cancer cell lines, namely CT26, HT29, and MKN74. PTE showed a dose-dependent inhibition of cell proliferation, sphere-forming ability, and stem cell marker expression in all three cell lines. Furthermore, the cells treated with PTE showed an increase in mitochondrial membrane potential and an increase in mitochondrial oxidative stress and lipid peroxide. Upon concurrent treatment with vitamin E, N-acetyl-L-cysteine, and PTE, the PTE-induced mitochondrial oxidative stress and growth inhibition were suppressed. These findings indicate that PTE induces oxidative stress in cancer cells, suppresses stemness, and inhibits proliferation. These antitumor effects of PTE are considered to be useful in cancer treatment.https://www.mdpi.com/1422-0067/21/24/9347pterostilbenecancerstemnessoxidative stress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shiori Mori Shingo Kishi Kanya Honoki Rina Fujiwara-Tani Takuma Moriguchi Takamitsu Sasaki Kiyomu Fujii Shinji Tsukamoto Hiromasa Fujii Akira Kido Yasuhito Tanaka Yi Luo Hiroki Kuniyasu |
spellingShingle |
Shiori Mori Shingo Kishi Kanya Honoki Rina Fujiwara-Tani Takuma Moriguchi Takamitsu Sasaki Kiyomu Fujii Shinji Tsukamoto Hiromasa Fujii Akira Kido Yasuhito Tanaka Yi Luo Hiroki Kuniyasu Anti-Stem Cell Property of Pterostilbene in Gastrointestinal Cancer Cells International Journal of Molecular Sciences pterostilbene cancer stemness oxidative stress |
author_facet |
Shiori Mori Shingo Kishi Kanya Honoki Rina Fujiwara-Tani Takuma Moriguchi Takamitsu Sasaki Kiyomu Fujii Shinji Tsukamoto Hiromasa Fujii Akira Kido Yasuhito Tanaka Yi Luo Hiroki Kuniyasu |
author_sort |
Shiori Mori |
title |
Anti-Stem Cell Property of Pterostilbene in Gastrointestinal Cancer Cells |
title_short |
Anti-Stem Cell Property of Pterostilbene in Gastrointestinal Cancer Cells |
title_full |
Anti-Stem Cell Property of Pterostilbene in Gastrointestinal Cancer Cells |
title_fullStr |
Anti-Stem Cell Property of Pterostilbene in Gastrointestinal Cancer Cells |
title_full_unstemmed |
Anti-Stem Cell Property of Pterostilbene in Gastrointestinal Cancer Cells |
title_sort |
anti-stem cell property of pterostilbene in gastrointestinal cancer cells |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-12-01 |
description |
Pterostilbene (PTE) is a natural sterbenoid contained in blueberries that has an antioxidant effect. In contrast, PTE also generates oxidative stress in cancer cells and provides an antitumor effect. Here, we examined the potential mechanism of this contrasting effect of PTE using three gastrointestinal cancer cell lines, namely CT26, HT29, and MKN74. PTE showed a dose-dependent inhibition of cell proliferation, sphere-forming ability, and stem cell marker expression in all three cell lines. Furthermore, the cells treated with PTE showed an increase in mitochondrial membrane potential and an increase in mitochondrial oxidative stress and lipid peroxide. Upon concurrent treatment with vitamin E, N-acetyl-L-cysteine, and PTE, the PTE-induced mitochondrial oxidative stress and growth inhibition were suppressed. These findings indicate that PTE induces oxidative stress in cancer cells, suppresses stemness, and inhibits proliferation. These antitumor effects of PTE are considered to be useful in cancer treatment. |
topic |
pterostilbene cancer stemness oxidative stress |
url |
https://www.mdpi.com/1422-0067/21/24/9347 |
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