A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)
Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identifica...
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doaj-50a62ba2106447058af07661bee026082021-02-23T09:15:09ZengBMCBMC Biotechnology1472-67502021-02-012111910.1186/s12896-021-00676-9A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)Sujit Shah0Krishna Chand1Bhagwan Rekadwad2Yogesh S. Shouche3Jyotsna Sharma4Bijaya Pant5Central Department of Botany, Tribhuvan UniversityCentral Department of Botany, Tribhuvan UniversityCurrent Address: Yenepoya Research Centre, Yenepoya (Deemed to be University)National Centre for Microbial Resource, National Centre for Cell Science, Savitribai Phule Pune University CampusDepartment of Plant Science and Soil Science, Texas Tech UniversityCentral Department of Botany, Tribhuvan UniversityAbstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.https://doi.org/10.1186/s12896-021-00676-9EndophytesIAAOrchidVanda cristataSymbiosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sujit Shah Krishna Chand Bhagwan Rekadwad Yogesh S. Shouche Jyotsna Sharma Bijaya Pant |
spellingShingle |
Sujit Shah Krishna Chand Bhagwan Rekadwad Yogesh S. Shouche Jyotsna Sharma Bijaya Pant A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata) BMC Biotechnology Endophytes IAA Orchid Vanda cristata Symbiosis |
author_facet |
Sujit Shah Krishna Chand Bhagwan Rekadwad Yogesh S. Shouche Jyotsna Sharma Bijaya Pant |
author_sort |
Sujit Shah |
title |
A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata) |
title_short |
A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata) |
title_full |
A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata) |
title_fullStr |
A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata) |
title_full_unstemmed |
A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata) |
title_sort |
prospectus of plant growth promoting endophytic bacterium from orchid (vanda cristata) |
publisher |
BMC |
series |
BMC Biotechnology |
issn |
1472-6750 |
publishDate |
2021-02-01 |
description |
Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment. |
topic |
Endophytes IAA Orchid Vanda cristata Symbiosis |
url |
https://doi.org/10.1186/s12896-021-00676-9 |
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