Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process

• Main Authors: Nayara Bezerra Carvalho, José Murillo P. Barbosa, Maria Vanessa S. Oliveira, Alini T. Fricks, Álvaro S. Lima, Cleide Mara F. Soares
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2013-01-01
• Series: Química Nova
• Subjects: biochemical; lipase from Bacillus immobilized; sol gel process
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422013000100010

This work presents biochemical characterization of a lipase from a new strain of Bacillus sp. ITP-001, immobilized using a sol gel process (IB). The results from the biochemical characterization of IB showed increased activity for hydrolysis, with 526.63 U g-1 at pH 5.0 and 80 ºC, and thermal stability at 37 ºC. Enzymatic activity was stimulated by ions such as EDTA, Fe+3, Mn+2, Zn+2, and Ca+2, and in various organic solvents. Kinetic parameters obtained for the IB were Km = 14.62 mM, and Vmax = 0.102 mM min-1 g-1. The results of biochemical characterization revealed the improved catalytic properties of IB.