The endemic Helicobacter pylori population in Southern Vietnam has both South East Asian and European origins
Abstract Background The burden of Helicobacter pylori-induced gastric cancer varies based on predominant H. pylori population in various geographical regions. Vietnam is a high H. pylori burden country with the highest age-standardized incidence rate of gastric cancer (16.3 cases/100,000 for both se...
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doaj-50b1446af3b94230af082a9c906e98472021-10-03T11:20:54ZengBMCGut Pathogens1757-47492021-09-0113111410.1186/s13099-021-00452-2The endemic Helicobacter pylori population in Southern Vietnam has both South East Asian and European originsTrang Hoa Nguyen0Trang Thi My Ho1Thien-Phuc Nguyen-Hoang2Shamsul Qumar3Thuc Tran Dang Pham4Quy Nhuan Bui5Dieter Bulach6Thuy-Vy Nguyen7Motiur Rahman8Oxford University Clinical Research UnitDepartment of Genetics, Faculty of Biology and Biotechnology, Ho Chi Minh University of ScienceDepartment of Genetics, Faculty of Biology and Biotechnology, Ho Chi Minh University of ScienceGeneStore India Pvt LtdDepartment of Genetics, Faculty of Biology and Biotechnology, Ho Chi Minh University of ScienceDepartment of Gastroenterology, Gia Dinh HospitalMelbourne Bioinformatics, The University of Melbourne and Doherty Applied Microbial Genomics, The Doherty InstituteDepartment of Genetics, Faculty of Biology and Biotechnology, Ho Chi Minh University of ScienceOxford University Clinical Research UnitAbstract Background The burden of Helicobacter pylori-induced gastric cancer varies based on predominant H. pylori population in various geographical regions. Vietnam is a high H. pylori burden country with the highest age-standardized incidence rate of gastric cancer (16.3 cases/100,000 for both sexes) in Southeast Asia, despite this data on the H. pylori population is scanty. We examined the global context of the endemic H. pylori population in Vietnam and present a contextual and comparative genomics analysis of 83 H. pylori isolates from patients in Vietnam. Results There are at least two major H. pylori populations are circulating in symptomatic Vietnamese patients. The majority of the isolates (~ 80%, 66/83) belong to the hspEastAsia and the remaining belong to hpEurope population (~ 20%, 17/83). In total, 66 isolates (66/83) were cagA positive, 64 were hspEastAsia isolates and two were hpEurope isolates. Examination of the second repeat region revealed that most of the cagA genes were ABD type (63/66; 61 were hspEastAsia isolates and two were hpEurope isolates). The remaining three isolates (all from hspEastAsia isolates) were ABC or ABCC types. We also detected that 4.5% (3/66) cagA gene from hspEastAsia isolates contained EPIYA-like sequences, ESIYA at EPIYA-B segments. Analysis of the vacA allelic type revealed 98.8% (82/83) and 41% (34/83) of the strains harboured the s1 and m1 allelic variant, respectively; 34/83 carried both s1m1 alleles. The most frequent genotypes among the cagA positive isolates were vacA s1m1/cagA + and vacA s1m2/cagA + , accounting for 51.5% (34/66) and 48.5% (32/66) of the isolates, respectively. Conclusions There are two predominant lineages of H. pylori circulating in Vietnam; most of the isolates belong to the hspEastAsia population. The hpEurope population is further divided into two smaller clusters.https://doi.org/10.1186/s13099-021-00452-2H. pyloriVietnamMolecular epidemiology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Trang Hoa Nguyen Trang Thi My Ho Thien-Phuc Nguyen-Hoang Shamsul Qumar Thuc Tran Dang Pham Quy Nhuan Bui Dieter Bulach Thuy-Vy Nguyen Motiur Rahman |
spellingShingle |
Trang Hoa Nguyen Trang Thi My Ho Thien-Phuc Nguyen-Hoang Shamsul Qumar Thuc Tran Dang Pham Quy Nhuan Bui Dieter Bulach Thuy-Vy Nguyen Motiur Rahman The endemic Helicobacter pylori population in Southern Vietnam has both South East Asian and European origins Gut Pathogens H. pylori Vietnam Molecular epidemiology |
author_facet |
Trang Hoa Nguyen Trang Thi My Ho Thien-Phuc Nguyen-Hoang Shamsul Qumar Thuc Tran Dang Pham Quy Nhuan Bui Dieter Bulach Thuy-Vy Nguyen Motiur Rahman |
author_sort |
Trang Hoa Nguyen |
title |
The endemic Helicobacter pylori population in Southern Vietnam has both South East Asian and European origins |
title_short |
The endemic Helicobacter pylori population in Southern Vietnam has both South East Asian and European origins |
title_full |
The endemic Helicobacter pylori population in Southern Vietnam has both South East Asian and European origins |
title_fullStr |
The endemic Helicobacter pylori population in Southern Vietnam has both South East Asian and European origins |
title_full_unstemmed |
The endemic Helicobacter pylori population in Southern Vietnam has both South East Asian and European origins |
title_sort |
endemic helicobacter pylori population in southern vietnam has both south east asian and european origins |
publisher |
BMC |
series |
Gut Pathogens |
issn |
1757-4749 |
publishDate |
2021-09-01 |
description |
Abstract Background The burden of Helicobacter pylori-induced gastric cancer varies based on predominant H. pylori population in various geographical regions. Vietnam is a high H. pylori burden country with the highest age-standardized incidence rate of gastric cancer (16.3 cases/100,000 for both sexes) in Southeast Asia, despite this data on the H. pylori population is scanty. We examined the global context of the endemic H. pylori population in Vietnam and present a contextual and comparative genomics analysis of 83 H. pylori isolates from patients in Vietnam. Results There are at least two major H. pylori populations are circulating in symptomatic Vietnamese patients. The majority of the isolates (~ 80%, 66/83) belong to the hspEastAsia and the remaining belong to hpEurope population (~ 20%, 17/83). In total, 66 isolates (66/83) were cagA positive, 64 were hspEastAsia isolates and two were hpEurope isolates. Examination of the second repeat region revealed that most of the cagA genes were ABD type (63/66; 61 were hspEastAsia isolates and two were hpEurope isolates). The remaining three isolates (all from hspEastAsia isolates) were ABC or ABCC types. We also detected that 4.5% (3/66) cagA gene from hspEastAsia isolates contained EPIYA-like sequences, ESIYA at EPIYA-B segments. Analysis of the vacA allelic type revealed 98.8% (82/83) and 41% (34/83) of the strains harboured the s1 and m1 allelic variant, respectively; 34/83 carried both s1m1 alleles. The most frequent genotypes among the cagA positive isolates were vacA s1m1/cagA + and vacA s1m2/cagA + , accounting for 51.5% (34/66) and 48.5% (32/66) of the isolates, respectively. Conclusions There are two predominant lineages of H. pylori circulating in Vietnam; most of the isolates belong to the hspEastAsia population. The hpEurope population is further divided into two smaller clusters. |
topic |
H. pylori Vietnam Molecular epidemiology |
url |
https://doi.org/10.1186/s13099-021-00452-2 |
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