Terbutaline, forskolin and cAMP reduce secretion of aqueous humour in the isolated bovine eye.
In order to elucidate involvement of cyclic AMP and intracellular Ca2+,[Ca2+]i, in the modulation of aqueous humour formation (AHF), we studied the effects of terbutaline, forskolin and 8-Br-cAMP in the isolated bovine eye. We also studied the interaction of cAMP on calcium signaling in cultured cil...
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doaj-50e03901951b4ae382f0b0b8d68cfde42021-03-12T05:30:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011512e024425310.1371/journal.pone.0244253Terbutaline, forskolin and cAMP reduce secretion of aqueous humour in the isolated bovine eye.Mohammad ShahidullahWilliam Stuart WilsonKazi RafiqMahmudul Hasan SikderJannatul FerdousNicholas Anthony DelamereIn order to elucidate involvement of cyclic AMP and intracellular Ca2+,[Ca2+]i, in the modulation of aqueous humour formation (AHF), we studied the effects of terbutaline, forskolin and 8-Br-cAMP in the isolated bovine eye. We also studied the interaction of cAMP on calcium signaling in cultured ciliary epithelial (CE) cells. Drug effects on AHF were measured by fluorescein dilution. Drug effects on [Ca2+]i were studied by the fura-2 fluorescence ratio technique. Terbutaline (100 nmol-100 M), forskolin (30 nM-100 M) or 8-Br-cAMP (100 nM- 10 μM), administered in the arterial perfusate produced significant reductions in AHF. The AH reducing effect of terbutaline was blocked by a selective inhibitor of protein kinase A (KT-5720). ATP (100 M) caused a rapid, transient (peak) increase in [Ca2+]i followed by a sustained plateau phase lasting more than 5 minutes. Preincubation of the cells (6 min) with terbutaline, forskolin or 8-Br-cAMP significantly reduced the peak calcium response to ATP. The sustained plateau phase of the response, on the other hand, was augmented by each of the agents. KT-5720 partially reversed the inhibitory effect of terbutaline on the peak and totally inhibited its effect on the plateau phase. These data indicate: (a) that AHF in the bovine eye can be manipulated through cyclic AMP, operating via protein kinase A, (b) that protein kinase A can affect [Ca2+]i homeostasis, (c) that calcium release from the intracellular store, not the entry, affects AHF, and (d) that interaction of [Ca2+]i with cAMP plays a role in modulating AH secretion.https://doi.org/10.1371/journal.pone.0244253 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohammad Shahidullah William Stuart Wilson Kazi Rafiq Mahmudul Hasan Sikder Jannatul Ferdous Nicholas Anthony Delamere |
spellingShingle |
Mohammad Shahidullah William Stuart Wilson Kazi Rafiq Mahmudul Hasan Sikder Jannatul Ferdous Nicholas Anthony Delamere Terbutaline, forskolin and cAMP reduce secretion of aqueous humour in the isolated bovine eye. PLoS ONE |
author_facet |
Mohammad Shahidullah William Stuart Wilson Kazi Rafiq Mahmudul Hasan Sikder Jannatul Ferdous Nicholas Anthony Delamere |
author_sort |
Mohammad Shahidullah |
title |
Terbutaline, forskolin and cAMP reduce secretion of aqueous humour in the isolated bovine eye. |
title_short |
Terbutaline, forskolin and cAMP reduce secretion of aqueous humour in the isolated bovine eye. |
title_full |
Terbutaline, forskolin and cAMP reduce secretion of aqueous humour in the isolated bovine eye. |
title_fullStr |
Terbutaline, forskolin and cAMP reduce secretion of aqueous humour in the isolated bovine eye. |
title_full_unstemmed |
Terbutaline, forskolin and cAMP reduce secretion of aqueous humour in the isolated bovine eye. |
title_sort |
terbutaline, forskolin and camp reduce secretion of aqueous humour in the isolated bovine eye. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2020-01-01 |
description |
In order to elucidate involvement of cyclic AMP and intracellular Ca2+,[Ca2+]i, in the modulation of aqueous humour formation (AHF), we studied the effects of terbutaline, forskolin and 8-Br-cAMP in the isolated bovine eye. We also studied the interaction of cAMP on calcium signaling in cultured ciliary epithelial (CE) cells. Drug effects on AHF were measured by fluorescein dilution. Drug effects on [Ca2+]i were studied by the fura-2 fluorescence ratio technique. Terbutaline (100 nmol-100 M), forskolin (30 nM-100 M) or 8-Br-cAMP (100 nM- 10 μM), administered in the arterial perfusate produced significant reductions in AHF. The AH reducing effect of terbutaline was blocked by a selective inhibitor of protein kinase A (KT-5720). ATP (100 M) caused a rapid, transient (peak) increase in [Ca2+]i followed by a sustained plateau phase lasting more than 5 minutes. Preincubation of the cells (6 min) with terbutaline, forskolin or 8-Br-cAMP significantly reduced the peak calcium response to ATP. The sustained plateau phase of the response, on the other hand, was augmented by each of the agents. KT-5720 partially reversed the inhibitory effect of terbutaline on the peak and totally inhibited its effect on the plateau phase. These data indicate: (a) that AHF in the bovine eye can be manipulated through cyclic AMP, operating via protein kinase A, (b) that protein kinase A can affect [Ca2+]i homeostasis, (c) that calcium release from the intracellular store, not the entry, affects AHF, and (d) that interaction of [Ca2+]i with cAMP plays a role in modulating AH secretion. |
url |
https://doi.org/10.1371/journal.pone.0244253 |
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