Assessment of Dental Caries Experience Along WithStreptococcus mutans, Streptococcus sanguis and CandidaCount among Male Tobacco Users Visiting a Dental Instituteof Mangalore, Karnataka, India: A Cross-sectional Study
Aim: The aim of this study was to compare the dental caries experience and count of Streptococcus mutans, Streptococcus sanguis and Candida among tobacco users visiting a Dental Institute of Mangalore. Materials and Methods: 75 subjects were taken for the study, from this 24 were tobacco smokers,...
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doaj-50edbfb5bec24401bef1c85e07f87f3d2020-11-24T21:23:09ZengAmber PublicationJournal of Research in Medical and Dental Science2347-25452347-23672019-02-0171131135Assessment of Dental Caries Experience Along WithStreptococcus mutans, Streptococcus sanguis and CandidaCount among Male Tobacco Users Visiting a Dental Instituteof Mangalore, Karnataka, India: A Cross-sectional StudyVijaya Hegde0 Sharanya Sreekumar1Department of Public Health Dentistry, AJ Institute of Dental Sciences, Mangalore, IndiaDepartment of Public Health Dentistry, AJ Institute of Dental Sciences, Mangalore, IndiaAim: The aim of this study was to compare the dental caries experience and count of Streptococcus mutans, Streptococcus sanguis and Candida among tobacco users visiting a Dental Institute of Mangalore. Materials and Methods: 75 subjects were taken for the study, from this 24 were tobacco smokers, 27 smokeless tobacco users and 24 were tobacco non-users. Dental caries experiences among these subjects were taken using DMFT index and their saliva was collected. Stimulated saliva was taken about 2.5 ml in sterile plastic container and sent to Department of Microbiology. Saliva was inoculated in MSA medium for mutans, sanguis and sabouround’s agar medium for candida at 37°C. The candida growth appeared in 48 hours as cream/white isolated colonies and mutans, sanguis growth appear in 24 hours as tiny light blue and large blue colonies which is counted using digital colony counter machine. Results: The mean DMFT among smokers were 4.63, smokeless were 2.22 and non-users were 1.79. The mean CFU of Streptococcus mutans and Streptococcus sanguis among smokers, smokeless users and non-users were 46 × 104, 6.27 × 104, 3.85 × 104 and 10 × 104, 2.03 × 104, 1.3 × 104 respectively. The mean CFU of candida among smokers, smokeless users and non-users were 133.33, 7.78 and 4.17 respectively. Conclusion: Thus it can be concluded that dental caries experience and growth of microbes was found higher among smokers compared to smokeless users and non-users. The dental caries experience among smokeless users were slightly higher compared to non-users but not statistically significant.https://www.jrmds.in/articles/assessment-of-dental-caries-experience-along-with-streptococcus-mutans-streptococcus-sanguis-and-candida-count-among-male-tobacco-.pdfStreptococcus mutansStreptococcus sanguisCandidaTobacco smokersTobacco smokeless users |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vijaya Hegde Sharanya Sreekumar |
spellingShingle |
Vijaya Hegde Sharanya Sreekumar Assessment of Dental Caries Experience Along WithStreptococcus mutans, Streptococcus sanguis and CandidaCount among Male Tobacco Users Visiting a Dental Instituteof Mangalore, Karnataka, India: A Cross-sectional Study Journal of Research in Medical and Dental Science Streptococcus mutans Streptococcus sanguis Candida Tobacco smokers Tobacco smokeless users |
author_facet |
Vijaya Hegde Sharanya Sreekumar |
author_sort |
Vijaya Hegde |
title |
Assessment of Dental Caries Experience Along WithStreptococcus mutans, Streptococcus sanguis and CandidaCount among Male Tobacco Users Visiting a Dental Instituteof Mangalore, Karnataka, India: A Cross-sectional Study |
title_short |
Assessment of Dental Caries Experience Along WithStreptococcus mutans, Streptococcus sanguis and CandidaCount among Male Tobacco Users Visiting a Dental Instituteof Mangalore, Karnataka, India: A Cross-sectional Study |
title_full |
Assessment of Dental Caries Experience Along WithStreptococcus mutans, Streptococcus sanguis and CandidaCount among Male Tobacco Users Visiting a Dental Instituteof Mangalore, Karnataka, India: A Cross-sectional Study |
title_fullStr |
Assessment of Dental Caries Experience Along WithStreptococcus mutans, Streptococcus sanguis and CandidaCount among Male Tobacco Users Visiting a Dental Instituteof Mangalore, Karnataka, India: A Cross-sectional Study |
title_full_unstemmed |
Assessment of Dental Caries Experience Along WithStreptococcus mutans, Streptococcus sanguis and CandidaCount among Male Tobacco Users Visiting a Dental Instituteof Mangalore, Karnataka, India: A Cross-sectional Study |
title_sort |
assessment of dental caries experience along withstreptococcus mutans, streptococcus sanguis and candidacount among male tobacco users visiting a dental instituteof mangalore, karnataka, india: a cross-sectional study |
publisher |
Amber Publication |
series |
Journal of Research in Medical and Dental Science |
issn |
2347-2545 2347-2367 |
publishDate |
2019-02-01 |
description |
Aim: The aim of this study was to compare the dental caries experience and count of Streptococcus mutans, Streptococcus sanguis and Candida among tobacco users visiting a Dental Institute of Mangalore.
Materials and Methods: 75 subjects were taken for the study, from this 24 were tobacco smokers, 27 smokeless tobacco users and 24 were tobacco non-users. Dental caries experiences among these subjects were taken using DMFT index and their saliva was collected. Stimulated saliva was taken about 2.5 ml in sterile plastic container and sent to Department of Microbiology. Saliva was inoculated in MSA medium for mutans, sanguis and sabouround’s agar medium for candida at 37°C. The candida growth appeared in 48 hours as cream/white isolated colonies and mutans, sanguis growth appear in 24 hours as tiny light blue and large blue colonies which is counted using digital colony counter machine.
Results: The mean DMFT among smokers were 4.63, smokeless were 2.22 and non-users were 1.79. The mean CFU of Streptococcus mutans and Streptococcus sanguis among smokers, smokeless users and non-users were 46 × 104, 6.27 × 104, 3.85 × 104 and 10 × 104, 2.03 × 104, 1.3 × 104 respectively. The mean CFU of candida among smokers, smokeless users and non-users were 133.33, 7.78 and 4.17 respectively.
Conclusion: Thus it can be concluded that dental caries experience and growth of microbes was found higher among smokers compared to smokeless users and non-users. The dental caries experience among smokeless users were slightly higher compared to non-users but not statistically significant. |
topic |
Streptococcus mutans Streptococcus sanguis Candida Tobacco smokers Tobacco smokeless users |
url |
https://www.jrmds.in/articles/assessment-of-dental-caries-experience-along-with-streptococcus-mutans-streptococcus-sanguis-and-candida-count-among-male-tobacco-.pdf |
work_keys_str_mv |
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