An activity of caspase-3 and cathepsin D at the different subtypes of ischemic stroke
<p class="a"><strong>Aim of research</strong> – To define the dynamics of activity of caspase-3, cathepsin D, apoptosis of leukocytes at the different subtypes of ischemic stroke (IS) in an acute period.</p><p class="a"><strong>Methods</stro...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
PC Technology Center
2015-06-01
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Series: | ScienceRise |
Subjects: | |
Online Access: | http://journals.uran.ua/sciencerise/article/view/45158 |
Summary: | <p class="a"><strong>Aim of research</strong> – To define the dynamics of activity of caspase-3, cathepsin D, apoptosis of leukocytes at the different subtypes of ischemic stroke (IS) in an acute period.</p><p class="a"><strong>Methods</strong><strong>.</strong> There were examined 232 patients in an acute period of ІS: 56 (24,1%)- with hemodynamic (HDS), 62 (2,.7%) – with atherothrombotic (АТS), 60 (25,9%) – with cardioembolic (CЕS) і 54 (23,3%) – with lacunar stroke (LS). There was defined the number of leukocytes at the stage of apoptosis (ANV<sup>+</sup>-cells), necrosis (PI<sup>+</sup>-cells), with an increased content of the active forms of oxygen (AFO<sup>+</sup>-cells) and with lowered mitochondrial potential (Mito<sup>+</sup>-cells), activity of caspase-3 and cathepsin D.</p><p class="a"><strong>Results</strong>. It was established that at all subtypes of IS mitochondrial dysfunction, apoptosis and necrosis of leukocytes are observed on the 1<sup>st</sup> day it were presented in increase of content of ANV<sup>+</sup>-, PI<sup>+</sup>-, АFO<sup>+</sup>- and Mito<sup>+</sup>-cells and were the mostly apparent at ATS. The highest activity of caspase-3 on the 1<sup>st</sup> day was noticed at LS it did not correlate with a number of cells at the stage of apoptosis and probably was connected with a predominant impact of caspase-3 on endothelium and with hyperpermeability of hematoencephalic barrier. In patients with ATS an activity of cathepsin D increased during the 1<sup>st</sup> week of disease that can indicate an activation of lysosomal way of activation of apoptosis that courses parallel to an apoptosis connected with mitochondrial dysfunction.</p><p class="a"><strong>Conclusions</strong><strong>.</strong> The different ways of apoptotic cellular death that depends on subtype of stroke activate in an acute period of IS</p> |
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ISSN: | 2313-6286 2313-8416 |