An activity of caspase-3 and cathepsin D at the different subtypes of ischemic stroke

• Main Authors: Наталія Романівна Сохор, Світлана Іванівна Шкробот, Олена Юріївна Бударна, Оксана Романівна Ясний
• Format: Article
• Language: English
• Published: PC Technology Center 2015-06-01
• Series: ScienceRise
• Subjects: acute period of ischemic stroke; apoptosis; caspase-3; cathepsin D
• Online Access: http://journals.uran.ua/sciencerise/article/view/45158
• ISSN: 2313-6286; 2313-8416

<p class="a"><strong>Aim of research</strong> – To define the dynamics of activity of caspase-3, cathepsin D, apoptosis of leukocytes at the different subtypes of ischemic stroke (IS) in an acute period.</p><p class="a"><strong>Methods</strong><strong>.</strong> There were examined 232 patients in an acute period of ІS: 56 (24,1%)- with hemodynamic (HDS), 62 (2,.7%) – with atherothrombotic (АТS), 60 (25,9%) – with cardioembolic (CЕS) і 54 (23,3%) – with lacunar stroke (LS). There was defined the number of leukocytes at the stage of apoptosis (ANV⁺-cells), necrosis (PI⁺-cells), with an increased content of the active forms of oxygen (AFO⁺-cells) and with lowered mitochondrial potential (Mito⁺-cells), activity of caspase-3 and cathepsin D.</p><p class="a"><strong>Results</strong>. It was established that at all subtypes of IS mitochondrial dysfunction, apoptosis and necrosis of leukocytes are observed on the 1^st day it were presented in increase of content of  ANV⁺-, PI⁺-, АFO⁺- and Mito⁺-cells and were the mostly apparent at ATS.   The highest activity of caspase-3 on the 1^st day was noticed at LS it did not correlate with a number of cells at the stage of apoptosis and probably was connected with a predominant impact of caspase-3 on endothelium and with hyperpermeability of hematoencephalic barrier. In patients with ATS an activity of cathepsin D increased during the 1^st week of disease that can indicate an activation of lysosomal way of activation of apoptosis that courses parallel to an apoptosis connected with mitochondrial dysfunction.</p><p class="a"><strong>Conclusions</strong><strong>.</strong>  The different ways of apoptotic cellular death that depends on subtype of stroke activate in an acute period of IS</p>