The new genomic editing system (CRISPR)
Over the past decades, progression in genetic element manipulation, and consequently, the treatment of diseases has been remarkable. It is worth noting that these genetic manipulations perform at different levels, including DNA and RNA. The earlier genomic editing techniques, including MN, ZFN , TAL...
Main Authors: | , |
---|---|
Format: | Article |
Language: | fas |
Published: |
Shahid Sadoughi University of Medical Sciences
2019-10-01
|
Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
Subjects: | |
Online Access: | http://jssu.ssu.ac.ir/article-1-4615-en.html |
id |
doaj-51033ebbc1b54fc9a482f4d555bd052f |
---|---|
record_format |
Article |
spelling |
doaj-51033ebbc1b54fc9a482f4d555bd052f2020-11-25T01:12:55ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332019-10-0127515681583The new genomic editing system (CRISPR)Mehrdad Talebi0Mohammad Yahya Vahidi Mehrjardi1 Department of Medical Genetics- Shahid Sadoughi University of Medical Sciences Diabetes Research Center- Shahid Sadoughi University of Medical Sciences Over the past decades, progression in genetic element manipulation, and consequently, the treatment of diseases has been remarkable. It is worth noting that these genetic manipulations perform at different levels, including DNA and RNA. The earlier genomic editing techniques, including MN, ZFN , TALEN , performing their functions by creating double-stranded breaks (DSBs), and after breakage, the cell tries to repair the breakage through two systems, homologous recombination and non-homologous end joining. CRISPR/Cas technology has been discovered recently, and has become the most widely used genome-editing tool, mainly due to its capabilities and those added to this through the genetic engineering. In this study, we aimed to introduce a variety of CRISPR classes in the elementary parts, and then the modified CRISPR systems developed to increase the efficiency and specificity of the system and provide acceptable results will be introduced. In this study, for three months in the fall and winter, Pubmed and Web of science sites searched for keywords such as CRISPR, Types of CRISPR, gRNA, Cas9, and CRISPR-Cas9 nickase that eventually resulted in about four hundred Sixty-one articles, and some of these articles after closer study, reviewed in this article. Genetic engineering techniques have successfully transformed this system into the most efficient genome editing tool in recent years. Researchers are working on a system to treat various diseases by resolving problems such as high specificity, cutting off non-target sites, how to move to a cell, and setting up a proper repair system.http://jssu.ssu.ac.ir/article-1-4615-en.html1-Genome editing techniquesCRISPRHomologous recombinationNon-homologous end joining. |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Mehrdad Talebi Mohammad Yahya Vahidi Mehrjardi |
spellingShingle |
Mehrdad Talebi Mohammad Yahya Vahidi Mehrjardi The new genomic editing system (CRISPR) Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd 1-Genome editing techniques CRISPR Homologous recombination Non-homologous end joining. |
author_facet |
Mehrdad Talebi Mohammad Yahya Vahidi Mehrjardi |
author_sort |
Mehrdad Talebi |
title |
The new genomic editing system (CRISPR) |
title_short |
The new genomic editing system (CRISPR) |
title_full |
The new genomic editing system (CRISPR) |
title_fullStr |
The new genomic editing system (CRISPR) |
title_full_unstemmed |
The new genomic editing system (CRISPR) |
title_sort |
new genomic editing system (crispr) |
publisher |
Shahid Sadoughi University of Medical Sciences |
series |
Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
issn |
2228-5741 2228-5733 |
publishDate |
2019-10-01 |
description |
Over the past decades, progression in genetic element manipulation, and consequently, the treatment of diseases has been remarkable. It is worth noting that these genetic manipulations perform at different levels, including DNA and RNA. The earlier genomic editing techniques, including MN, ZFN , TALEN , performing their functions by creating double-stranded breaks (DSBs), and after breakage, the cell tries to repair the breakage through two systems, homologous recombination and non-homologous end joining. CRISPR/Cas technology has been discovered recently, and has become the most widely used genome-editing tool, mainly due to its capabilities and those added to this through the genetic engineering. In this study, we aimed to introduce a variety of CRISPR classes in the elementary parts, and then the modified CRISPR systems developed to increase the efficiency and specificity of the system and provide acceptable results will be introduced.
In this study, for three months in the fall and winter, Pubmed and Web of science sites searched for keywords such as CRISPR, Types of CRISPR, gRNA, Cas9, and CRISPR-Cas9 nickase that eventually resulted in about four hundred Sixty-one articles, and some of these articles after closer study, reviewed in this article.
Genetic engineering techniques have successfully transformed this system into the most efficient genome editing tool in recent years. Researchers are working on a system to treat various diseases by resolving problems such as high specificity, cutting off non-target sites, how to move to a cell, and setting up a proper repair system. |
topic |
1-Genome editing techniques CRISPR Homologous recombination Non-homologous end joining. |
url |
http://jssu.ssu.ac.ir/article-1-4615-en.html |
work_keys_str_mv |
AT mehrdadtalebi thenewgenomiceditingsystemcrispr AT mohammadyahyavahidimehrjardi thenewgenomiceditingsystemcrispr AT mehrdadtalebi newgenomiceditingsystemcrispr AT mohammadyahyavahidimehrjardi newgenomiceditingsystemcrispr |
_version_ |
1725164378459734016 |