Galectin 3 and non-classical monocytes of blood as myocardial remodeling factors at ischemic cardiomyopathy
Aims: To identify an imbalance of cardiac remodeling mediators and monocytes subpopulation in blood, distribution of myocardium macrophages in patients with ischemic cardiomyopathy (ICMP). Methods: The study engaged 30 patients with ICMP, 26 patients with coronary heart disease (CHD) without ICMP, 1...
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doaj-5111969355944991a1891563db0065f02021-05-26T04:28:00ZengElsevierInternational Journal of Cardiology: Heart & Vasculature2352-90672021-04-0133100766Galectin 3 and non-classical monocytes of blood as myocardial remodeling factors at ischemic cardiomyopathyS. Chumakova0O. Urazova1V. Shipulin2M. Vins3A. Pryakhin4I. Sukhodolo5A. Stelmashenko6L. Litvinova7Yu. Kolobovnikova8E. Churina9V. Novitskiy10Pathophysiology Division of Siberian State Medical University, 2 Moskovsky Trakt, Tomsk 634050, Russia; Corresponding author at: 53 Tverskaya Street, Tomsk 634061, Russia.Pathophysiology Division of Siberian State Medical University, 2 Moskovsky Trakt, Tomsk 634050, RussiaCardiovascular Surgery Unit of CardiologyResearchInstitute, Tomsk National Medical Research Center of Russian Academy of Sciences, 111A Kievskaya Street, Tomsk 634012, RussiaPathophysiology Division of Siberian State Medical University, 2 Moskovsky Trakt, Tomsk 634050, RussiaCardiovascular Surgery Unit of CardiologyResearchInstitute, Tomsk National Medical Research Center of Russian Academy of Sciences, 111A Kievskaya Street, Tomsk 634012, RussiaMorphology Division of Siberian State Medical University, 2 Moskovsky Trakt, Tomsk 634050, RussiaMorphology Division of Siberian State Medical University, 2 Moskovsky Trakt, Tomsk 634050, RussiaImmunology and Cell Biotechnology Center of Immanuel Kant, Baltic Federal University, 14 A. Nevskogo Street, Kaliningrad 236041, RussiaPathophysiology Division of Siberian State Medical University, 2 Moskovsky Trakt, Tomsk 634050, RussiaPathophysiology Division of Siberian State Medical University, 2 Moskovsky Trakt, Tomsk 634050, Russia; National Research Tomsk State University, 36 Lenina Ave, Tomsk 634050, RussiaPathophysiology Division of Siberian State Medical University, 2 Moskovsky Trakt, Tomsk 634050, RussiaAims: To identify an imbalance of cardiac remodeling mediators and monocytes subpopulation in blood, distribution of myocardium macrophages in patients with ischemic cardiomyopathy (ICMP). Methods: The study engaged 30 patients with ICMP, 26 patients with coronary heart disease (CHD) without ICMP, 15 healthy donors. Concentrations of TGFβ, MMP-9, MCP-1, galectin-3 were measured in plasma of blood from the coronary sinus and peripheral blood in CHD patients, as well as in peripheral blood in healthy donors, by enzyme immunoassay method. The ration of classical, intermediate, non-classical, transitional monocytes in peripheral blood of patients and healthy donors was assessed by flow cytometry (expression CD14, CD16); the content of CD68+ macrophages in myocardium – by immunohistochemistry method. Results: In both samples of blood, the content of galectin-3 in patients with ICMP was higher than in CHD patients without ICMP and the level of TGFβ was comparable between the groups. At ICMP, the concentration of MMP-9 in sinus blood was higher than that in CHD patients without ICMP in whom an excess of MCP-1 in the general blood flow was determined. The density of distribution of CD68+ cells in the myocardium in patients with ICMP was higher in the perianeurysmal zone than in the right atrium appendage. ICMP was characterized by a deficiency of non-classical monocytes, and CHD without ICMP – by an excess of intermediate cells in peripheral blood. Conclusion: Myocardium remodeling at ICMP is mediated by not so much TGFβ but intracardiac galectin-3, which determines the subpopulation composition of blood monocytes.http://www.sciencedirect.com/science/article/pii/S2352906721000543Galectin-3Transforming growth factorFibrosisMonocyte subpopulationsMacrophagesIschemic cardiomyopathy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
S. Chumakova O. Urazova V. Shipulin M. Vins A. Pryakhin I. Sukhodolo A. Stelmashenko L. Litvinova Yu. Kolobovnikova E. Churina V. Novitskiy |
spellingShingle |
S. Chumakova O. Urazova V. Shipulin M. Vins A. Pryakhin I. Sukhodolo A. Stelmashenko L. Litvinova Yu. Kolobovnikova E. Churina V. Novitskiy Galectin 3 and non-classical monocytes of blood as myocardial remodeling factors at ischemic cardiomyopathy International Journal of Cardiology: Heart & Vasculature Galectin-3 Transforming growth factor Fibrosis Monocyte subpopulations Macrophages Ischemic cardiomyopathy |
author_facet |
S. Chumakova O. Urazova V. Shipulin M. Vins A. Pryakhin I. Sukhodolo A. Stelmashenko L. Litvinova Yu. Kolobovnikova E. Churina V. Novitskiy |
author_sort |
S. Chumakova |
title |
Galectin 3 and non-classical monocytes of blood as myocardial remodeling factors at ischemic cardiomyopathy |
title_short |
Galectin 3 and non-classical monocytes of blood as myocardial remodeling factors at ischemic cardiomyopathy |
title_full |
Galectin 3 and non-classical monocytes of blood as myocardial remodeling factors at ischemic cardiomyopathy |
title_fullStr |
Galectin 3 and non-classical monocytes of blood as myocardial remodeling factors at ischemic cardiomyopathy |
title_full_unstemmed |
Galectin 3 and non-classical monocytes of blood as myocardial remodeling factors at ischemic cardiomyopathy |
title_sort |
galectin 3 and non-classical monocytes of blood as myocardial remodeling factors at ischemic cardiomyopathy |
publisher |
Elsevier |
series |
International Journal of Cardiology: Heart & Vasculature |
issn |
2352-9067 |
publishDate |
2021-04-01 |
description |
Aims: To identify an imbalance of cardiac remodeling mediators and monocytes subpopulation in blood, distribution of myocardium macrophages in patients with ischemic cardiomyopathy (ICMP). Methods: The study engaged 30 patients with ICMP, 26 patients with coronary heart disease (CHD) without ICMP, 15 healthy donors. Concentrations of TGFβ, MMP-9, MCP-1, galectin-3 were measured in plasma of blood from the coronary sinus and peripheral blood in CHD patients, as well as in peripheral blood in healthy donors, by enzyme immunoassay method. The ration of classical, intermediate, non-classical, transitional monocytes in peripheral blood of patients and healthy donors was assessed by flow cytometry (expression CD14, CD16); the content of CD68+ macrophages in myocardium – by immunohistochemistry method. Results: In both samples of blood, the content of galectin-3 in patients with ICMP was higher than in CHD patients without ICMP and the level of TGFβ was comparable between the groups. At ICMP, the concentration of MMP-9 in sinus blood was higher than that in CHD patients without ICMP in whom an excess of MCP-1 in the general blood flow was determined. The density of distribution of CD68+ cells in the myocardium in patients with ICMP was higher in the perianeurysmal zone than in the right atrium appendage. ICMP was characterized by a deficiency of non-classical monocytes, and CHD without ICMP – by an excess of intermediate cells in peripheral blood. Conclusion: Myocardium remodeling at ICMP is mediated by not so much TGFβ but intracardiac galectin-3, which determines the subpopulation composition of blood monocytes. |
topic |
Galectin-3 Transforming growth factor Fibrosis Monocyte subpopulations Macrophages Ischemic cardiomyopathy |
url |
http://www.sciencedirect.com/science/article/pii/S2352906721000543 |
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