A Preclinical Porcine Model of Portal Vein Thrombosis in Liver Cirrhosis
Background. This study aimed at presenting a novel method of developing a porcine model of portal vein thrombosis (PVT) in cirrhosis by intravenous administration of thrombin and insertion of a fibered coil. We further investigated changes of biochemical parameters, coagulation, and proinflammatory...
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2020-01-01
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Online Access: | http://dx.doi.org/10.1155/2020/3086906 |
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doaj-511d712a6d4941eba2d23d9fd1e01c662020-11-25T02:54:34ZengHindawi LimitedBioMed Research International2314-61332314-61412020-01-01202010.1155/2020/30869063086906A Preclinical Porcine Model of Portal Vein Thrombosis in Liver CirrhosisRui Zhang0Shenxin Lu1Ying-yi Jiang2Jing-qin Ma3Wen Zhang4Jun-ying Gu5Jian Wang6Shi-yao Chen7Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai, ChinaLiver Cancer Institute, Zhongshan Hospital, Fudan University and Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, ChinaDepartment of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Interventional Radiology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Interventional Radiology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Radiology, Zhongshan Hospital, Shanghai, ChinaDepartment of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai, ChinaBackground. This study aimed at presenting a novel method of developing a porcine model of portal vein thrombosis (PVT) in cirrhosis by intravenous administration of thrombin and insertion of a fibered coil. We further investigated changes of biochemical parameters, coagulation, and proinflammatory cytokine expression in the cirrhosis-PVT group. Methods. Twelve male pigs were randomized into the control group (n=3) and cirrhosis group (n=9). In cirrhotic pigs, three were randomly selected to establish PVT by ultrasound-guided percutaneous puncture of the main portal vein (MPV) followed by intravenous thrombin administration and fibered coil insertion. Thrombosis in the MPV was detected by abdominal enhanced computer tomography (CT). The changes of hepatic function, coagulation system, and inflammation cytokines were compared among normal, cirrhosis, and cirrhosis with PVT groups. Results. As manifested by the presence of a filling defect in MPV on portal venous-phase CT angiography, fibrin thrombi were formed in the MPV in cirrhotic pigs within one week and persisted for four weeks. Five weeks after surgery, abnormal liver functions occurred in association with PVT formation in cirrhosis. Both coagulation and thromboelastography parameters showed that cirrhosis-PVT pigs exhibited a procoagulant state through hyperfunction of platelets and clotting factors. Interleukin 6 (IL-6) as a potential inflammatory marker stimulated PVT-mediated inflammation activation in cirrhosis. Conclusions. Our study provides in vivo evidence that intravenous injection of a coil and thrombin into MPV under interventional guided devices enables a feasible method in thrombus creation. Further exploration and validation of large-sample cases are required to characterize utilities of this model.http://dx.doi.org/10.1155/2020/3086906 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rui Zhang Shenxin Lu Ying-yi Jiang Jing-qin Ma Wen Zhang Jun-ying Gu Jian Wang Shi-yao Chen |
spellingShingle |
Rui Zhang Shenxin Lu Ying-yi Jiang Jing-qin Ma Wen Zhang Jun-ying Gu Jian Wang Shi-yao Chen A Preclinical Porcine Model of Portal Vein Thrombosis in Liver Cirrhosis BioMed Research International |
author_facet |
Rui Zhang Shenxin Lu Ying-yi Jiang Jing-qin Ma Wen Zhang Jun-ying Gu Jian Wang Shi-yao Chen |
author_sort |
Rui Zhang |
title |
A Preclinical Porcine Model of Portal Vein Thrombosis in Liver Cirrhosis |
title_short |
A Preclinical Porcine Model of Portal Vein Thrombosis in Liver Cirrhosis |
title_full |
A Preclinical Porcine Model of Portal Vein Thrombosis in Liver Cirrhosis |
title_fullStr |
A Preclinical Porcine Model of Portal Vein Thrombosis in Liver Cirrhosis |
title_full_unstemmed |
A Preclinical Porcine Model of Portal Vein Thrombosis in Liver Cirrhosis |
title_sort |
preclinical porcine model of portal vein thrombosis in liver cirrhosis |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2020-01-01 |
description |
Background. This study aimed at presenting a novel method of developing a porcine model of portal vein thrombosis (PVT) in cirrhosis by intravenous administration of thrombin and insertion of a fibered coil. We further investigated changes of biochemical parameters, coagulation, and proinflammatory cytokine expression in the cirrhosis-PVT group. Methods. Twelve male pigs were randomized into the control group (n=3) and cirrhosis group (n=9). In cirrhotic pigs, three were randomly selected to establish PVT by ultrasound-guided percutaneous puncture of the main portal vein (MPV) followed by intravenous thrombin administration and fibered coil insertion. Thrombosis in the MPV was detected by abdominal enhanced computer tomography (CT). The changes of hepatic function, coagulation system, and inflammation cytokines were compared among normal, cirrhosis, and cirrhosis with PVT groups. Results. As manifested by the presence of a filling defect in MPV on portal venous-phase CT angiography, fibrin thrombi were formed in the MPV in cirrhotic pigs within one week and persisted for four weeks. Five weeks after surgery, abnormal liver functions occurred in association with PVT formation in cirrhosis. Both coagulation and thromboelastography parameters showed that cirrhosis-PVT pigs exhibited a procoagulant state through hyperfunction of platelets and clotting factors. Interleukin 6 (IL-6) as a potential inflammatory marker stimulated PVT-mediated inflammation activation in cirrhosis. Conclusions. Our study provides in vivo evidence that intravenous injection of a coil and thrombin into MPV under interventional guided devices enables a feasible method in thrombus creation. Further exploration and validation of large-sample cases are required to characterize utilities of this model. |
url |
http://dx.doi.org/10.1155/2020/3086906 |
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