Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle Against Carbapenem-Resistant Acinetobacter baumannii
Acinetobacter baumannii is a multi-drug resistant opportunistic pathogen, which causes respiratory and urinary tract infections. Its prevalence increases gradually in the clinical setup. Carbapenems (beta-lactam) are most effective antibiotics till now against A. baumannii, but the development of re...
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doaj-514f6e3439c0427a8cf8fae78f8768d42020-11-24T22:02:25ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-06-01910.3389/fmicb.2018.01218362763Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle Against Carbapenem-Resistant Acinetobacter baumanniiVishvanath Tiwari0Neha Mishra1Keval Gadani2P. S. Solanki3N. A. Shah4Monalisa Tiwari5Department of Biochemistry, Central University of Rajasthan, Ajmer, IndiaDepartment of Biochemistry, Central University of Rajasthan, Ajmer, IndiaDepartment of Physics, Saurashtra University, Rajkot, IndiaDepartment of Physics, Saurashtra University, Rajkot, IndiaDepartment of Physics, Saurashtra University, Rajkot, IndiaDepartment of Biochemistry, Central University of Rajasthan, Ajmer, IndiaAcinetobacter baumannii is a multi-drug resistant opportunistic pathogen, which causes respiratory and urinary tract infections. Its prevalence increases gradually in the clinical setup. Carbapenems (beta-lactam) are most effective antibiotics till now against A. baumannii, but the development of resistance against it may lead to high mortality. Therefore, it is of utmost importance to develop an alternative drug against A. baumannii. In the present study, we have synthesized ZnO nanoparticle (ZnO-NP) and characterized by X-ray diffraction, Fourier transform infrared (FTIR) spectroscopy and UV-Visible spectroscopy. Prepared ZnO-NPs have the size of 30 nm and have different characteristics of ZnO-NPs. Growth kinetics and disk diffusion assay showed that ZnO-NP demonstrated good antibacterial activity against carbapenem resistant A. baumannii. We have also investigated the mechanism of action of ZnO-NPs on the carbapenem resistant strain of A. baumannii. The proposed mechanism of action of ZnO involves the production of reactive oxygen species, which elevates membrane lipid peroxidation that causes membrane leakage of reducing sugars, DNA, proteins, and reduces cell viability. These results demonstrate that ZnO-NP could be developed as alternative therapeutics against A. baumannii.https://www.frontiersin.org/article/10.3389/fmicb.2018.01218/fullAcinetobacter baumanniimicrobial drug resistanceantimicrobial nanoparticlesmechanism of ZnO actionROS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vishvanath Tiwari Neha Mishra Keval Gadani P. S. Solanki N. A. Shah Monalisa Tiwari |
spellingShingle |
Vishvanath Tiwari Neha Mishra Keval Gadani P. S. Solanki N. A. Shah Monalisa Tiwari Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle Against Carbapenem-Resistant Acinetobacter baumannii Frontiers in Microbiology Acinetobacter baumannii microbial drug resistance antimicrobial nanoparticles mechanism of ZnO action ROS |
author_facet |
Vishvanath Tiwari Neha Mishra Keval Gadani P. S. Solanki N. A. Shah Monalisa Tiwari |
author_sort |
Vishvanath Tiwari |
title |
Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle Against Carbapenem-Resistant Acinetobacter baumannii |
title_short |
Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle Against Carbapenem-Resistant Acinetobacter baumannii |
title_full |
Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle Against Carbapenem-Resistant Acinetobacter baumannii |
title_fullStr |
Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle Against Carbapenem-Resistant Acinetobacter baumannii |
title_full_unstemmed |
Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle Against Carbapenem-Resistant Acinetobacter baumannii |
title_sort |
mechanism of anti-bacterial activity of zinc oxide nanoparticle against carbapenem-resistant acinetobacter baumannii |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-06-01 |
description |
Acinetobacter baumannii is a multi-drug resistant opportunistic pathogen, which causes respiratory and urinary tract infections. Its prevalence increases gradually in the clinical setup. Carbapenems (beta-lactam) are most effective antibiotics till now against A. baumannii, but the development of resistance against it may lead to high mortality. Therefore, it is of utmost importance to develop an alternative drug against A. baumannii. In the present study, we have synthesized ZnO nanoparticle (ZnO-NP) and characterized by X-ray diffraction, Fourier transform infrared (FTIR) spectroscopy and UV-Visible spectroscopy. Prepared ZnO-NPs have the size of 30 nm and have different characteristics of ZnO-NPs. Growth kinetics and disk diffusion assay showed that ZnO-NP demonstrated good antibacterial activity against carbapenem resistant A. baumannii. We have also investigated the mechanism of action of ZnO-NPs on the carbapenem resistant strain of A. baumannii. The proposed mechanism of action of ZnO involves the production of reactive oxygen species, which elevates membrane lipid peroxidation that causes membrane leakage of reducing sugars, DNA, proteins, and reduces cell viability. These results demonstrate that ZnO-NP could be developed as alternative therapeutics against A. baumannii. |
topic |
Acinetobacter baumannii microbial drug resistance antimicrobial nanoparticles mechanism of ZnO action ROS |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2018.01218/full |
work_keys_str_mv |
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