Transketolase and vitamin B1 influence on ROS-dependent neutrophil extracellular traps (NETs) formation.
Neutrophil extracellular traps (NETs) are a recently identified, web-like, extracellular structure composed of decondensed nuclear DNA and associated antimicrobial granules. NETs are extruded into the extracellular environment via the reactive oxygen species (ROS)-dependent cell death pathway partic...
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doaj-5165f9a526304a13980e940f56f62cf62021-03-03T20:33:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01148e022101610.1371/journal.pone.0221016Transketolase and vitamin B1 influence on ROS-dependent neutrophil extracellular traps (NETs) formation.Donporn RiyapaDarawan RinchaiVeerachat MuangsombutChayanin WuttinontananchaiMohammed ToufiqDamien ChaussabelManabu AtoJenefer M BlackwellSunee KorbsrisateNeutrophil extracellular traps (NETs) are a recently identified, web-like, extracellular structure composed of decondensed nuclear DNA and associated antimicrobial granules. NETs are extruded into the extracellular environment via the reactive oxygen species (ROS)-dependent cell death pathway participating in inflammation and autoimmune diseases. Transketolase (TKT) is a thiamine pyrophosphate (vitamin B1)-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway by feeding excess sugar phosphates into the main carbohydrate metabolic pathways to generate biosynthetic reducing capacity in the form of NADPH as a substrate for ROS generation. In this work, TKT was selected as a lead candidate from 24 NET-associated proteins obtained by literature screening and knowledge gap assessment. Consequently, we determined whether TKT influenced NET formation in vitro. We firstly established that the release of ROS-dependent NETs was significantly decreased after purified human PMNs were pretreated with oxythiamine, a TKT inhibitor, and in a concentration dependent manner. As a cofactor for TKT reaction, we evaluated the release of NET formation either in vitamin B1 treatment or in combined use of oxythiamine and vitamin B1, and found that those treatments also exerted a significant suppressive effect on the amount of NET-DNA and ROS production. The regulation of TKT by oxythiamine and/or vitamin B1 may therefore be associated with response to the modulation of NET formation by preventing generation of excessive NETs in inflammatory diseases.https://doi.org/10.1371/journal.pone.0221016 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Donporn Riyapa Darawan Rinchai Veerachat Muangsombut Chayanin Wuttinontananchai Mohammed Toufiq Damien Chaussabel Manabu Ato Jenefer M Blackwell Sunee Korbsrisate |
spellingShingle |
Donporn Riyapa Darawan Rinchai Veerachat Muangsombut Chayanin Wuttinontananchai Mohammed Toufiq Damien Chaussabel Manabu Ato Jenefer M Blackwell Sunee Korbsrisate Transketolase and vitamin B1 influence on ROS-dependent neutrophil extracellular traps (NETs) formation. PLoS ONE |
author_facet |
Donporn Riyapa Darawan Rinchai Veerachat Muangsombut Chayanin Wuttinontananchai Mohammed Toufiq Damien Chaussabel Manabu Ato Jenefer M Blackwell Sunee Korbsrisate |
author_sort |
Donporn Riyapa |
title |
Transketolase and vitamin B1 influence on ROS-dependent neutrophil extracellular traps (NETs) formation. |
title_short |
Transketolase and vitamin B1 influence on ROS-dependent neutrophil extracellular traps (NETs) formation. |
title_full |
Transketolase and vitamin B1 influence on ROS-dependent neutrophil extracellular traps (NETs) formation. |
title_fullStr |
Transketolase and vitamin B1 influence on ROS-dependent neutrophil extracellular traps (NETs) formation. |
title_full_unstemmed |
Transketolase and vitamin B1 influence on ROS-dependent neutrophil extracellular traps (NETs) formation. |
title_sort |
transketolase and vitamin b1 influence on ros-dependent neutrophil extracellular traps (nets) formation. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
Neutrophil extracellular traps (NETs) are a recently identified, web-like, extracellular structure composed of decondensed nuclear DNA and associated antimicrobial granules. NETs are extruded into the extracellular environment via the reactive oxygen species (ROS)-dependent cell death pathway participating in inflammation and autoimmune diseases. Transketolase (TKT) is a thiamine pyrophosphate (vitamin B1)-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway by feeding excess sugar phosphates into the main carbohydrate metabolic pathways to generate biosynthetic reducing capacity in the form of NADPH as a substrate for ROS generation. In this work, TKT was selected as a lead candidate from 24 NET-associated proteins obtained by literature screening and knowledge gap assessment. Consequently, we determined whether TKT influenced NET formation in vitro. We firstly established that the release of ROS-dependent NETs was significantly decreased after purified human PMNs were pretreated with oxythiamine, a TKT inhibitor, and in a concentration dependent manner. As a cofactor for TKT reaction, we evaluated the release of NET formation either in vitamin B1 treatment or in combined use of oxythiamine and vitamin B1, and found that those treatments also exerted a significant suppressive effect on the amount of NET-DNA and ROS production. The regulation of TKT by oxythiamine and/or vitamin B1 may therefore be associated with response to the modulation of NET formation by preventing generation of excessive NETs in inflammatory diseases. |
url |
https://doi.org/10.1371/journal.pone.0221016 |
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