Fabrication of 3D Micro-Blades for the Cutting of Biological Structures in a Microfluidic Guillotine
Micro-blade design is an important factor in the cutting of single cells and other biological structures. This paper describes the fabrication process of three-dimensional (3D) micro-blades for the cutting of single cells in a microfluidic “guillotine” intended for fundamental wound repair and regen...
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MDPI AG
2021-08-01
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doaj-516fa0685e2045cc80adf75e23e14aa12021-09-26T00:42:22ZengMDPI AGMicromachines2072-666X2021-08-01121005100510.3390/mi12091005Fabrication of 3D Micro-Blades for the Cutting of Biological Structures in a Microfluidic GuillotineSaisneha Koppaka0Kevin S. Zhang1Myra Kurosu Jalil2Lucas R. Blauch3Sindy K. Y. Tang4Department of Mechanical Engineering, Stanford University, Stanford, CA 94305, USADepartment of Mechanical Engineering, Stanford University, Stanford, CA 94305, USADepartment of Mechanical Engineering, Stanford University, Stanford, CA 94305, USADepartment of Mechanical Engineering, Stanford University, Stanford, CA 94305, USADepartment of Mechanical Engineering, Stanford University, Stanford, CA 94305, USAMicro-blade design is an important factor in the cutting of single cells and other biological structures. This paper describes the fabrication process of three-dimensional (3D) micro-blades for the cutting of single cells in a microfluidic “guillotine” intended for fundamental wound repair and regeneration studies. Our microfluidic guillotine consists of a fixed 3D micro-blade centered in a microchannel to bisect cells flowing through. We show that the Nanoscribe two-photon polymerization direct laser writing system is capable of fabricating complex 3D micro-blade geometries. However, structures made of the Nanoscribe IP-S resin have low adhesion to silicon, and they tend to peel off from the substrate after at most two times of replica molding in poly(dimethylsiloxane) (PDMS). Our work demonstrates that the use of a secondary mold replicates Nanoscribe-printed features faithfully for at least 10 iterations. Finally, we show that complex micro-blade features can generate different degrees of cell wounding and cell survival rates compared with simple blades possessing a vertical cutting edge fabricated with conventional 2.5D photolithography. Our work lays the foundation for future applications in single cell analyses, wound repair and regeneration studies, as well as investigations of the physics of cutting and the interaction between the micro-blade and biological structures.https://www.mdpi.com/2072-666X/12/9/10053D printingmicrofabricationmicrofluidic guillotinesingle cellwound healing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Saisneha Koppaka Kevin S. Zhang Myra Kurosu Jalil Lucas R. Blauch Sindy K. Y. Tang |
spellingShingle |
Saisneha Koppaka Kevin S. Zhang Myra Kurosu Jalil Lucas R. Blauch Sindy K. Y. Tang Fabrication of 3D Micro-Blades for the Cutting of Biological Structures in a Microfluidic Guillotine Micromachines 3D printing microfabrication microfluidic guillotine single cell wound healing |
author_facet |
Saisneha Koppaka Kevin S. Zhang Myra Kurosu Jalil Lucas R. Blauch Sindy K. Y. Tang |
author_sort |
Saisneha Koppaka |
title |
Fabrication of 3D Micro-Blades for the Cutting of Biological Structures in a Microfluidic Guillotine |
title_short |
Fabrication of 3D Micro-Blades for the Cutting of Biological Structures in a Microfluidic Guillotine |
title_full |
Fabrication of 3D Micro-Blades for the Cutting of Biological Structures in a Microfluidic Guillotine |
title_fullStr |
Fabrication of 3D Micro-Blades for the Cutting of Biological Structures in a Microfluidic Guillotine |
title_full_unstemmed |
Fabrication of 3D Micro-Blades for the Cutting of Biological Structures in a Microfluidic Guillotine |
title_sort |
fabrication of 3d micro-blades for the cutting of biological structures in a microfluidic guillotine |
publisher |
MDPI AG |
series |
Micromachines |
issn |
2072-666X |
publishDate |
2021-08-01 |
description |
Micro-blade design is an important factor in the cutting of single cells and other biological structures. This paper describes the fabrication process of three-dimensional (3D) micro-blades for the cutting of single cells in a microfluidic “guillotine” intended for fundamental wound repair and regeneration studies. Our microfluidic guillotine consists of a fixed 3D micro-blade centered in a microchannel to bisect cells flowing through. We show that the Nanoscribe two-photon polymerization direct laser writing system is capable of fabricating complex 3D micro-blade geometries. However, structures made of the Nanoscribe IP-S resin have low adhesion to silicon, and they tend to peel off from the substrate after at most two times of replica molding in poly(dimethylsiloxane) (PDMS). Our work demonstrates that the use of a secondary mold replicates Nanoscribe-printed features faithfully for at least 10 iterations. Finally, we show that complex micro-blade features can generate different degrees of cell wounding and cell survival rates compared with simple blades possessing a vertical cutting edge fabricated with conventional 2.5D photolithography. Our work lays the foundation for future applications in single cell analyses, wound repair and regeneration studies, as well as investigations of the physics of cutting and the interaction between the micro-blade and biological structures. |
topic |
3D printing microfabrication microfluidic guillotine single cell wound healing |
url |
https://www.mdpi.com/2072-666X/12/9/1005 |
work_keys_str_mv |
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1716870005820227584 |